We narrowed to 7,089 results for: Cad

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pcDNA3-eGFP-NLS-RNF168

Plasmid

#133977

Purpose

mammalian expression vector of eGFP tagged wild type RNF168

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Tags

eGFP

Expression

Mammalian

Mutation

siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

Promoter

CMV

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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His-FLAG-Tev_ACTB (beta-actin)

Plasmid

#188453

Purpose

Express in mammalian cells human beta-actin (ACTB) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

ACTB (ACTB Human)

Tags

6xHis-FLAG

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-Best1-sCD200

Plasmid

#164424

Purpose

AAV plasmid expressing soluble CD200 in retinal pigment epithelium

Depositor

Connie Cepko

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

Insert

CD200 (Cd200 Mouse)

Use

AAV

Expression

Mammalian

Mutation

deleted terminal 40 amino acids

Promoter

Best1

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMGF196

Plasmid

#97005

Purpose

Expresses LEMD2-mCherry in mammalian cells under a crippled promoter

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

LEMD2 (LEMD2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV delta 5

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 myc-Rab10

Plasmid

#208366

Purpose

Mammalian expression of myc-tagged human Rab10

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

myc-Rab10 (RAB10 Human)

Expression

Mammalian

Mutation

wild type

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-BFP-Mito

Plasmid

#237398

Purpose

Lentivirus plasmid for the stable expression of mitochondrial matrix-localized BFP in mammalian cells

Depositor

James Hurley

Article

Rose et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

Insert

COX8A (COX8A Human)

Use

Lentiviral

Tags

EBFP2

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pIRES2-DsRed-MST-Kv2.1

Plasmid

#131707

Purpose

Expresses Kv2.1in mammalian cells

Depositor

Jennifer Kearney

Article

Jorge et al Proc Natl Acad Sci U S A. 2011 Mar 29;10

Insert

KCNB1 (KCNB1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pET32a-HD46Q

Plasmid

#11515

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 46Q glutamines (HTT Human)

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 46 glutamines, vector m…

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV CD68-hM4D(Gi)-mCherry

Plasmid

#75033

Purpose

Expression of hM4D(Gi) using the CD68 promoter.

Depositor

Bryan Roth

Article

Grace et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Has Service

AAV1 and AAV9

Insert

hM4D(Gi)-mCherry

Use

AAV

Expression

Mammalian

Promoter

CD68

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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EXT1- D164A-pLJC2-3XFLAG

Plasmid

#215828

Purpose

Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

Depositor

Yoav Shaul

Article

Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

Exostosin glycosyltransferase 1 Mutant (EXT1 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

Plasmid

#106309

Purpose

The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.

Depositor

James Zhe Liu

Article

Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

Insert

6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)

Tags

6xflag-3xHaloTag(MtoL)

Expression

Mammalian

Promoter

EF1

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-hTBP

Plasmid

#171073

Purpose

Expresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBP

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

TATA-box-binding protein (TBP Human)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5 HA-TBRII (delta Cyt)

Plasmid

#14051

Depositor

Joan Massague

Article

Siegel et al Proc Natl Acad Sci U S A. 2003 Jul 8. 10

Insert

TGF beta rec II (delta cyt) (TGFBR2 Human)

Tags

HA (PYDVPDYA)

Expression

Mammalian

Mutation

Dominant negative mutant. A stop codon was introd…

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

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pHR-ZIP(WT)

Plasmid

#27134

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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WIW_Scaffold2

Plasmid

#205574

Purpose

Nanobody that inhibits seeding of tau fibrils

Depositor

David Eisenberg

Article

Abskharon et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

WIW_Scaffold2

Tags

6X His

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pmNeon Green-N1-ARF1-R

Plasmid

#202430

Purpose

Mammalian expression vector containing mNeonGreen-tagged ARF1

Depositor

David Bryant

Article

Sandilands et al J Cell Biol. 2023 Apr 3;222(4):e20220611

Insert

ARF1 (ARF1 Human)

Use

Lentiviral

Tags

mNeonGreen

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-MDA5-S88D

Plasmid

#174226

Purpose

Expresses human MDA5 S88D mutant

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MDA5 (IFIH1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S88D

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR1A FKBP12F36V-OGT

Plasmid

#154287

Purpose

Entry vector encoding FKBP12(F36V)-2xHA-OGT (dTAG fused-O-GlcNAc Transferase)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x HA and FKBP12(F36V)

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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LYSO-LRRK2

Plasmid

#193582

Purpose

Expresses human full-length LRRK2 with a lysosomal targeting sequence from LAMTOR1 (1-39aa) and 3xFLAG on the N-terminus

Depositor

Mark Cookson

Article

Kluss et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

Leucine-rich repeat kinase 2 (LRRK2 Human)

Tags

LAMTOR1(1-39aa) and 3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro

Plasmid

#31845

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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