166,027 results

1. pGEX-4T-1-3xFLAG-HSP27

   Plasmid

   #129229

   Purpose

   Encodes FLAG tagged human HSP27 for purification by GST affinity

   Depositor

   Kevin Janes

   Article

   Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

   Insert

   HSP27 (HSPB1 Human)
   

   Tags

   FLAG and GST

   Expression

   Bacterial

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSG5 Large T

   Plasmid

   #9053

   Depositor

   William Hahn

   Insert

   large T antigen (SV40gp6 simian virus)
   

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET23a-mUbc9

   Plasmid

   #53137

   Purpose

   E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   Ubc9 (Ube2i Mouse)
   

   Tags

   untagged

   Expression

   Bacterial

   Mutation

   protein sequence is identical to human

   Promoter

   T7

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(-) MID49 4xMyc Hisx6

   Plasmid

   #44596

   Depositor

   David Chan

   Article

   Loson et al Mol Biol Cell. 2013 Jan 2.

   Insert

   MiD49 (Mief2 Mouse)
   

   Tags

   4xMyc and 6xHis

   Expression

   Mammalian

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA6-N-Flag-SREBP2

   Plasmid

   #134284

   Purpose

   Expresses Flag-tagged SREBP2 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   SREBP2 (Srebf2 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mouse SREBP2 precursor

   Promoter

   CMV

   Available Since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_CDKN1A_WT

   Plasmid

   #82201

   Purpose

   Gateway Donor vector containing CDKN1A , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-HDAC6.DC-FLAG

   Plasmid

   #30483

   Depositor

   Tso-Pang Yao

   Article

   Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

   Insert

   HDAC6 (HDAC6 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Catalytically inactive mutant (H216/611A)

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-DDX3

   Plasmid

   #44975

   Depositor

   Robin Reed

   Article

   Lee et al Nucleic Acids Res. 2008 Aug;36(14):4708-

   Insert

   DDX3 (DDX3X Human)
   

   Tags

   HA and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPM_Pveg-sfgfp

   Plasmid

   #121503

   Purpose

   Integration vector that expresses gfp in S. mutans

   Depositor

   Robert Shields

   Article

   Shields et al Appl Environ Microbiol. 2019 May 17. pii

   Insert

   Superfolder GFP
   

   Use

   S. mutans integration vector, integrates at the m…

   Promoter

   Pveg

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Mammalian expression: Z-lock cofilin dark state mutant

    Plasmid

    #141136

    Purpose

    a control for optogenetic cofilin analog

    Depositor

    Klaus Hahn

    Article

    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

    Insert

    Zdk2, Cofilin, LOV2
    

    Expression

    Mammalian

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. rGluA2 (px330:2-guideCas9)

    Plasmid

    #169439

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Paxillin-pEGFP

    Plasmid

    #15233

    Depositor

    Rick Horwitz

    Article

    Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

    Insert

    paxillin (PXN Chicken)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2

    Plasmid

    #205161

    Purpose

    express β-ACTIN-3′UTR-24xMS2

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    β-ACTIN-3′UTR-24xMS2
    

    Tags

    tagBFP

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMET7-GAG-EGFP

    Plasmid

    #80605

    Purpose

    Mammalian expression vector that expresses HIV-1 GAG with EGFP fused to it

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    SRalpha

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. GPI-mEGFP

    Plasmid

    #182866

    Purpose

    Monomer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    GPI-mEGFP
    

    Expression

    Mammalian

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. G555

    Plasmid

    #216483

    Purpose

    Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

    Depositor

    Christophe Danelon

    Insert

    plsB, plsC, cdsA, pssA
    

    Use

    Cell-free expression

    Promoter

    T7 for each gene

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-CXCL12-sfGFP

    Plasmid

    #98961

    Purpose

    Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCL12 (CXCL12 Human)
    

    Tags

    Superfolder GFP

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Fuw-dCas9-Tet1CD-P2A-BFP

    Plasmid

    #108245

    Purpose

    Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

    Insert

    dCas9-Tet1CD-HA-P2A-BFP
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    dCas9-D10A, dCas9-H840A

    Promoter

    Ubc

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-Akt1(WT)

    Plasmid

    #86637

    Purpose

    Expresses eGFP-tagged human Akt1(WT)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Expression

    Mammalian

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. His-GFP-Rab27a

    Plasmid

    #170861

    Purpose

    Mammalian expression of His-GFP-Rab27a.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    Rab-27a (RAB27A Human)
    

    Tags

    (His)6 and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGEX-KG-GST-YAP

    Plasmid

    #33052

    Depositor

    Kunliang Guan

    Article

    Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

    Insert

    YAP1 (YAP1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Available Since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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22. pRS316-RGR-GFP

    Plasmid

    #51056

    Purpose

    Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    Yeast ADH1 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. mscv2.2-NAIP5

    Plasmid

    #60205

    Purpose

    Mouse NAIP5 (B6 allele), expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of NAIP5

    Depositor

    Russell Vance

    Article

    Tenthorey et al Mol Cell. 2014 Apr 10;54(1):17-29. doi:

    Insert

    NAIP5
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pVax1-hVEGF165

    Plasmid

    #74466

    Purpose

    expression of hVEGF165 in mammalian cells

    Depositor

    Loree Heller

    Article

    Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

    Insert

    vascular endothelial growth factor 165 (VEGFA Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pHACK-GAL4>QF2

    Plasmid

    #80275

    Purpose

    GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.

    Depositor

    Christopher Potter

    Article

    Lin et al Genetics. 2016 Jun 22. pii: genetics.116

    Insert

    QF2
    

    Expression

    Insect

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pYPQ150

    Plasmid

    #69301

    Purpose

    Gateway entry vector with pcoCas9 (Plant codon optimized)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pcoCas9 (plant codon-optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Mutation

    NLS fusion

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pHAGE-ESR1

    Plasmid

    #116737

    Purpose

    Lentiviral expression of ESR1

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLVX-opto-hCaspase-4 dCARD

    Plasmid

    #208785

    Purpose

    Inducibly expresses human Caspase 4 lacking the CARD domain, fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Cry2 mCherry Caspase4 dCARD (CASP4 Human)
    

    Use

    Lentiviral

    Tags

    Cry2 mCherry

    Expression

    Mammalian

    Mutation

    CARD domain is removed

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. PJEBAN6

    Plasmid

    #203684

    Purpose

    Expresses red fluorescent marker DsRed-Express in Listeria monocytogenes

    Depositor

    Tine Rask Licht

    Article

    Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

    Insert

    DsRed-Express
    

    Expression

    Bacterial

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. GHRHR-Tango

    Plasmid

    #66292

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GHRHR (GHRHR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only