We narrowed to 11,261 results for: Kars
-
Plasmid#186872PurposeDoxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transductionDepositorInsertOrangutan cGAS truncation mutant (158-521 a.a.) with C-terminal HA tag (CGAS Pongo abelii, Synthetic)
UseRetroviralTagsHAMutationN-terminal truncationPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-human cGASdeltaN-HA
Plasmid#186843PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with C-terminal HA tag (Adamts1 Synthetic, Human)
UseRetroviralTagsHAMutationN-terminal truncationPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-orangutan cGASdeltaN-GFP
Plasmid#186846PurposeDoxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transductionDepositorInsertOrangutan cGAS truncation mutant (158-521 a.a.) with C-terminal GFP tag (CGAS Pongo abelii, Synthetic)
UseRetroviralTagsGFPMutationN-terminal truncationPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-chimpanzee cGASdeltaN-GFP
Plasmid#186847PurposeDoxycycline-dependent expression of chimpanzee cGAS gene in mammalian cells by retroviral transductionDepositorInsertChimpanzee cGAS truncation mutant (160-520 a.a.) with C-terminal GFP tag (CGAS Synthetic, Pan troglodytes)
UseRetroviralTagsGFPMutationN-terminal truncationPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-HA-human cGASdeltaN
Plasmid#186852PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with N-terminal HA tag (Adamts1 Synthetic, Human)
UseRetroviralTagsHAMutationN-terminal truncationPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-human cGASdeltaN
Plasmid#186854PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) without tags (Adamts1 Synthetic, Human)
UseRetroviralMutationN-terminal truncationPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-POLB
Plasmid#215138PurposeExpresses mCherry-POLB in mammalian cells from a retroviral vector.DepositorAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2826
Plasmid#143631PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2824
Plasmid#143630PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2827
Plasmid#143632PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV10-3xFLAG-prs-hMIGA2(170-593)-25GS-6xhis
Plasmid#192835PurposeExpress codon-optimized human MIGA2 fragment 170-593 with a GS-linker and 6xhis tag at C-terminus (lipid transfer construct) in Expi293 cellsDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
Tags3xFLAG-PreScission cleavage site and 6xhisExpressionMammalianPromoterCMVAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMECP2-RFP
Plasmid#181905PurposeFluorescently tagged MECP2, RFPDepositorAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-Exoc7-Ex5&10
Plasmid#133303PurposeEGFP reporter sequence was removed from TLCV2 vector and two gRNAs targeting mouse Exoc7 gene were cloned into the modified TLCV2 vector.DepositorInsertExocyst complex component 7 (Exoc7 Mouse)
UseCRISPR and LentiviralExpressionMammalianPromotermU6 promoterAvailable SinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh1
Plasmid#123324PurposeLentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insertDepositorInsertshRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh2
Plasmid#123325PurposeLentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insertDepositorInsertshRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA
Plasmid#182653PurposeEncodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
mutant eukaryotic release factor 1
TagsHA tag and nuclear export signal (NES)ExpressionMammalianMutationE55D and Y306A/Y384F (AF) double mutant of Methan…PromoterCMV and U6Available SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-LIG1
Plasmid#215131PurposeExpresses mCherry-LIG1 in mammalian cells from a retroviral vector.DepositorAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PCNA
Plasmid#215117PurposeExpresses mCherry-PCNA in mammalian cells from a lentiviral vector.DepositorAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-LIG3
Plasmid#215143PurposeExpresses mNeonGreen-LIG3 in mammalian cells from a retroviral vector.DepositorAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PARP1
Plasmid#215136PurposeExpresses mCherry-PARP1 in mammalian cells from a retroviral vector.DepositorAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5-EGFP-Tau AP
Plasmid#46905Purposeexpresses EGFP tagged Tau AP in mammalian cellsDepositorInsertTau AP (MAPT Human)
TagsEGFPExpressionMammalianMutationall 14 S/P or T/P amino acid residues (T111, T153…PromoterCMVAvailable SinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVX-FFSS-Cyclin D1
Plasmid#215151PurposeExpresses FFSS-Cyclin D1 in mammalian cells from a lentiviral vector.DepositorInsertCCND1 (CCND1 Human)
UseLentiviralTagsFFSS (FLAG-FLAG-STREP-STREP)ExpressionMammalianPromoterCMVAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti GW V5 Eco/Dam hum LaminB1
Plasmid#182671PurposeEukaryotic expression vector with E.Coli Dam methylation fused to human Lamin B1. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin B1.DepositorInsertLamin B1 (LMNB1 Human)
UseLentiviralTagsE. Coli Dam and V5ExpressionMammalianPromoterHSPAvailable SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-hNCL
Plasmid#181886PurposeSoluble light-dependent effector protein (nucleolin) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-hNCL (NCL Mustard Weed, Human)
TagsPHR-mCherryExpressionMammalianPromoterCMVAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-mHP1beta
Plasmid#181901PurposeFluorescently tagged HP1betaDepositorAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry
Plasmid#172620PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-OGG1
Plasmid#215135PurposeExpresses mCherry-OGG1 in mammalian cells from a retroviral vector.DepositorAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FEN1
Plasmid#215129PurposeExpresses mCherry-FEN1 in mammalian cells from a retroviral vector.DepositorAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-APEX1
Plasmid#215126PurposeExpresses mCherry-APEX1 in mammalian cells from a retroviral vector.DepositorAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
P527_POU4F2-p2A-mNeonGreen-h2b
Plasmid#239103PurposeAzenta NGS; inserts a p2A-mNeonGreen-h2b fusion at the endogenous POU4F2 (aka BRN3B) geneDepositorInsertPOU4F2 (POU4F2 Human)
Tagsp2A-mNeonGreen-h2bExpressionMammalianPromoterEndogenous POU4F2Available SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NLS-HA-TurboID-PCNA
Plasmid#215076PurposeExpresses NLS-HA-TurboID-PCNA in mammalian cells from a lentiviral vector.DepositorAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-YFP-KRAS4B
Plasmid#112718PurposeExpresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.DepositorInsertYFP-KRAS4B (KRAS Human)
ExpressionMammalianAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry
Plasmid#172627PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-2xFLAG-2xSTREP-CCND1-IRES-mCherry
Plasmid#172640PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1 and mCherry in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-POLE1
Plasmid#215140PurposeExpresses mCherry-POLE1 in mammalian cells from a retroviral vector.DepositorInsertPOLE1 (POLE Human)
UseRetroviralTagsmCherry_FWRExpressionMammalianMutationSilent mutations in amino acid 80-86 and 211-217 …PromoterCMVAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
P521_VSX2_p2A-h2b_mRuby3
Plasmid#239104PurposeUsed in CRISPR gene editing to Insert a p2A-h2b_mRuby3 sequence before the stop codon of the endogenous human VSX2 geneDepositorInsertVSX2 (VSX2 Human)
UseCRISPRTags-p2A-h2b-mRuby3ExpressionMammalianPromoterEndogenous VSX2 promoterAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2
Plasmid#202762PurposeEncodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harborDepositorAvailable SinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-HMBG1
Plasmid#215130PurposeExpresses mCherry-HMBG1 in mammalian cells from a retroviral vector.DepositorAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-SNAP-hCB2_IRES_EGFP
Plasmid#223506PurposeExpression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection markerDepositorAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-2xSTREP-CDKN1B-Puro
Plasmid#172611PurposeRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only