We narrowed to 17,206 results for: puro

1. pENN456_TALEcharm_Kv2_MmT8x

   Plasmid

   #220835

   Purpose

   TALEcharm expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   TALEcharm Kv2
   

   Use

   Lentiviral and TALEN

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EF1a

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM1-FLAG-metap2

   Plasmid

   #100510

   Purpose

   Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   METAP2 (METAP2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTargeting_Mettl3C_FKBP-V

   Plasmid

   #165421

   Purpose

   Template for knockin FBKP-V into Mettl3 C-terminus

   Depositor

   Neil Brockdorff

   Article

   Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

   Insert

   Mettl3 C-terminus + linker + FKBP-V (Mettl3 Mouse)
   

   Use

   Mouse Targeting

   Tags

   FKBP-V

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSMP-EZH2_1

   Plasmid

   #36387

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   EZH2 (EZH2 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPR-mCherry

   Plasmid

   #75161

   Purpose

   Derived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. TLCV2-APOBEC1-YTHmut

   Plasmid

   #178950

   Purpose

   Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

   Depositor

   Kate Meyer

   Article

   Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

   Insert

   APOBEC1-YTHmut-T2A-GFP
   

   Use

   Lentiviral and Synthetic Biology; Inducible

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRDA_355

   Plasmid

   #187159

   Purpose

   Lentiviral expression of doxycycline inducible customizable Sp Cas9 sgRNA

   Depositor

   David Root, John Doench

   Article

   Aquilanti et al Neuro Oncol. 2023 Jul 6;25(7):1275-1285.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMTL3

   Plasmid

   #127966

   Purpose

   constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   AAVS1-CAG-KRAB-dCas9-BFP-KRAB
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. W118-1_hTGM3-Flag

   Plasmid

   #180408

   Purpose

   lentiviral transduction of human TGM3 (with a C-terminal Flag tag) gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   TGM3 (TGM3 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMTL5

    Plasmid

    #127967

    Purpose

    DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX317-VRK1

    Plasmid

    #199643

    Purpose

    Expresses VRK1

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. bArr1-V5

    Plasmid

    #201492

    Purpose

    Expression of the beta-arrestin1 containing a C-terminal V5 tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    bArr1-V5 (ARRB1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRExT2A-CT-PmSc

    Plasmid

    #232549

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-PAC-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. W118-1_hTGM4-Flag

    Plasmid

    #180409

    Purpose

    lentiviral transduction of human TGM4 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM4 (TGM4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. W118-1_hTGM5-V5

    Plasmid

    #180414

    Purpose

    lentiviral transduction of human TGM5 (with a C-terminal V5 tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM5 (TGM5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SP-GFP-CD274-NU

    Plasmid

    #121139

    Purpose

    Mammalian expression of GFP tagged CD274

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Inserts

    CD274 (CD274 Human)
    CD274 (CD274 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 8522-M31-663

    Plasmid

    #225661

    Purpose

    Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (red configuration)

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    dsCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SORE6-mCMVp

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEAK-Flag/INPP4B

    Plasmid

    #24324

    Depositor

    Lewis Cantley

    Article

    Gewinner et al Cancer Cell. 2009 Aug 4. 16(2):115-25.

    Insert

    Inositol Polyphosphate 4-Phosphatase Type II (INPP4B Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    May 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMK395 (AAVS1 CMV-OsTIR1(F74A))

    Plasmid

    #159992

    Purpose

    Expression of OsTIR1(F74A) in human cells

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-OsTIR1(F74A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-eCas9

    Plasmid

    #140237

    Purpose

    Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a core

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PUC19_C_tag

    Plasmid

    #186659

    Purpose

    Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    C-terminal 3xFlag-3xHA Tag
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLX307-VHL(Y98H)-3F-BirA

    Plasmid

    #220148

    Purpose

    Expresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    von Hippel-Lindau tumor suppressor (VHL Human)
    

    Use

    Lentiviral

    Tags

    3F-BirA

    Expression

    Mammalian

    Mutation

    changed tyrosine 98 to histidine; codon optimized

    Promoter

    EF-1a

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p23-NES-LwaCas13a-msfGFP-NES-Flag

    Plasmid

    #165070

    Purpose

    overexpression of LwaCas13a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    LwaCas13a
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGL4_10xUAS-MLPmin-luc2

    Plasmid

    #194383

    Purpose

    Reporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoter

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    10xUAS-luc2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MLP minimal

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAS_8xMmaPylT_EF1_FLAG-MmaPylRS

    Plasmid

    #174890

    Purpose

    Mma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    van Husen et al Chembiochem. 2021 Aug 25. doi: 10.1002/c

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    U25C PylT

    Promoter

    EF1

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p23-NES-EsCas13d-msfGFP-NES-Flag

    Plasmid

    #165074

    Purpose

    overexpression of EsCas13d in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    EsCas13d
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMXS-IP APEX-PEX26

    Plasmid

    #224562

    Purpose

    expresses APEX fused to PEX26 peroxisomal localization sequence in mammalian cells

    Depositor

    William Kaelin, Gregory Wyant

    Article

    Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

    Insert

    PEX26 (PEX26 Human)
    

    Use

    Retroviral

    Tags

    APEX

    Expression

    Mammalian

    Mutation

    PEX26 peroxisomal targeting sequence

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGIPz-PD-L1 WT

    Plasmid

    #121486

    Purpose

    2nd generation lentiviral vector for over expressing human PD-L1 WT with PD-L1 shRNA

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLJC5-Flag-SAMTOR

    Plasmid

    #102420

    Purpose

    3rd generation lentiviral vector expressing FLAG SAMTOR in mammalian cells driven by UBC promoter

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    SAMTOR (BMT2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMXsIP GFP-VMP1

    Plasmid

    #117417

    Purpose

    Expresses VMP1 tagged with GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    VMP1 (VMP1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMXs-mPERK-FLAG-IP

    Plasmid

    #101793

    Purpose

    Retroviral vector encoding C-terminally FLAG tagged mouse PERK

    Depositor

    Seiichi Oyadomari

    Article

    Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

    Insert

    Perk (Eif2ak3 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSH-EFIRES-P-AtAFB2

    Plasmid

    #129715

    Purpose

    AAVS1 safe harbor targeting vector expressing AtAFB2

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    no

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF1a

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. dCas9-MSK1

    Plasmid

    #165601

    Purpose

    Expresses Sp dCas9 fused to human MSK1

    Depositor

    Isaac Hilton

    Article

    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

    Insert

    ribosomal protein S6 kinase A5 (RPS6KA5 S. Pyogenes, Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMXs-mHRI-FLAG-IP

    Plasmid

    #101791

    Purpose

    Retroviral vector encoding C-terminally FLAG tagged mouse HRI

    Depositor

    Seiichi Oyadomari

    Article

    Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

    Insert

    Hri (Eif2ak1 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSLQ1661-sgMUC4-E3(F+E)

    Plasmid

    #51025

    Purpose

    Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3

    Depositor

    Bo Huang, Stanley Qi

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    optimized sgRNA (MUC4 Mouse, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLVX-Tau-mCherry-RING

    Plasmid

    #233689

    Purpose

    Inducible expression of Tau-mCherry-RING

    Depositor

    Leo James

    Article

    Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

    Insert

    Tau-mCherry-RING
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGL4.20-HIF1A prom

    Plasmid

    #40173

    Depositor

    Alex Minella

    Article

    Sengupta et al Mol Cell Biol. 2011 Sep;31(18):3885-95.

    Insert

    HIF1A promoter fragment (HIF1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLENTI_RPS2_Keima

    Plasmid

    #127139

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS2_Keima (RPS2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLKO.1P shSOD1

    Plasmid

    #102975

    Purpose

    Suppression of SOD1

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shSOD1 (SOD1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MASTL B4.4 gRNA

    Plasmid

    #90755

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MASTL

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    MASTL (Guide Designation B4.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge