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pHBJT021

Plasmid

#225172

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (GmR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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ECFP-TevS-YPET

Plasmid

#100097

Purpose

FRET-based ECFP-YPET reporter that is cleaved by TEV

Depositor

Jim Wells

Article

Gray et al Cell. 2010 Aug 20;142(4):637-46. doi: 10

Insert

ECFP-TevS-YPET

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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M-SPOTIT1.1

Plasmid

#171216

Purpose

Expresses a mu-opioid receptor-based circular permuted green fluorescent protein sensor

Depositor

Wenjing Wang

Article

Kroning et al Angew Chem Int Ed Engl. 2021 Mar 4. doi:

Insert

M-SPOTIT1.1

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMBI

Plasmid

#17816

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
Opto-mFGFR1_204

Plasmid

#58745

Purpose

Expresses Opto-mFGFR1 (light-activated receptor tyrosine kinase)

Depositor

Harald Janovjak

Article

Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

Insert

Opto-mFGFR1

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSe_60

Plasmid

#109254

Purpose

TALEsp2 stabilized promoter driving GFP

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

TALEsp2 stabilized promoter

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB3236

Plasmid

#124708

Purpose

Expresses pTEF2-mKate2-tIDP1 and pTRX2_5xUAS-yEGFP-tCYC1 in yeast from plasdmid pRS416

Depositor

Michael Jensen

Article

Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

Insert

yEGFP

Promoter

pTRX2_5xUAS

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->HA-mtLaG2

Plasmid

#162225

Purpose

Lentiviral vector for the expression of HA GFP nanobody Lag2 in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-GFP nanobody LaG2

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR APP shRNA 2

Plasmid

#30135

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

APP shRNA 2

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pSFFV_CloGFP(1-10)

Plasmid

#157994

Purpose

Lentiviral vector of constitutive expression of CloGFP(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

CloGFP(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

G32Q, F46L, K101R, S202L

Promoter

SFFV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pTHSSe_59

Plasmid

#109253

Purpose

TALEsp1 stabilized promoter driving GFP

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

TALEsp1 stabilized promoter

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher-mi3

Plasmid

#112255

Purpose

Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

Insert

SpyCatcher-mi3

Tags

C-tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pKOS6b-KOpstB

Plasmid

#237501

Purpose

Knockout of pstB

Depositor

Buz Barstow

Insert

Phosphate ABC transporter ATP binding subunit

Expression

Bacterial

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pKOS6b-KOpstC

Plasmid

#237502

Purpose

Knockout of pstC

Depositor

Buz Barstow

Insert

Phosphate ABC transporter ATP binding subunit pstC

Expression

Bacterial

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcS-mCherry-C1C2

Plasmid

#178425

Purpose

Fluorescence reporter fused to EV membrane binding domain for EV imaging and tracking

Depositor

Masako Harada

Article

Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

Insert

mCherry

Expression

Mammalian

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHimar6

Plasmid

#137081

Purpose

bacterial Himar1 mini-transposon donor plasmid

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar1 mini-transposon with chloramphenicol resistance gene

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6p1-hPdcd4

Plasmid

#20693

Depositor

Haiwei Song

Article

Loh et al EMBO J. 2009 Feb 4. 28(3):274-85.

Insert

Programmed Cell Death Protein 4 (PDCD4 Human)

Tags

GST

Expression

Bacterial

Mutation

I36V- does not alter function

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
pHR_EF1a-->GssmycLaG16LaG2_P2A_NLSIFP

Plasmid

#162228

Purpose

Lentiviral vector for the expression of Myc GFP nanbody LaG16LaG2 with IFP NLS in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaG16LaG2 and IFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLV_CAG_Gag-MCP

Plasmid

#212637

Purpose

Lentivirus vector to express an MLV Gag-MCP fusion protein

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

MLV Gag (UniProt ID: P03332) (gag MoMLV)

Use

Lentiviral

Tags

MS2 Capsid Protein (N55K)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-iRFP720

Plasmid

#89687

Purpose

pCAG promoter driving iRFP720

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

iRFP720

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:GFP-CAAX

Plasmid

#184813

Purpose

Used to generate the Tg(QUAS:GFP-CAAX)c591 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GFP-CAAX-pA

Use

Zebrafish tol2 transgenesis

Tags

GFP-CAAX

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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PCDNA3-PAR1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

Plasmid

#113608

Purpose

PPI reporter

Depositor

Xiaokun Shu

Article

Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

Insert

PAR1

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pJW1219

Plasmid

#61250

Purpose

CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

sgRNA(F+E)

Use

CRISPR

Expression

Worm

Promoter

U6 promoter

Available Since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pRRL-MHCK7-R-GECO

Plasmid

#109374

Purpose

Expresses calcium indicator R-GECO in muscle cells

Depositor

Nenad Bursac

Article

Rao et al Nat Commun. 2018 Jan 9;9(1):126. doi: 10

Insert

R-GECO

Use

Lentiviral

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pST1374

Plasmid

#13426

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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SPS-6803

Plasmid

#80458

Purpose

IPTG inducible expressoin of sucrose phosphate synthase derived from Synechocystis sp. PCC6803

Depositor

Daniel Ducat

Article

Abramson et al Plant Cell Physiol. 2016 Oct 13. pii: pc

Insert

Sucrose phosphate synthase

Expression

Bacterial

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ134A

Plasmid

#69276

Purpose

Golden Gate entry vector; 4th gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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PCDNA3-S1P1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

Plasmid

#113612

Purpose

PPI reporter

Depositor

Xiaokun Shu

Article

Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

Insert

S1P1

Expression

Mammalian

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pET24b-BR1.4

Plasmid

#199436

Purpose

expresses BAmRed1.4 in bacterial cells

Depositor

Andrew Woolley

Article

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

BAMRed1.4

Tags

his tag

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBHM2619

Plasmid

#234498

Purpose

amdS-CnU6-empty sgRNA

Depositor

Hiten Madhani

Article

Nalley et al G3 (Bethesda). 2025 Aug 6;15(8):jkaf118.

Insert

amdS-PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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