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MSCV h c-MYC IRES GFP

Plasmid

#18119

Depositor

John Cleveland

Insert

c-MYC (MYC Human)

Use

Retroviral

Tags

GFP

Mutation

The CUG which is the start site for Myc I is pres…

Available Since

June 9, 2008

Availability

Academic Institutions and Nonprofits only

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CyRFP1-C1

Plasmid

#84354

Purpose

Expresses CyRFP1 fluorescent protein in an empty backbone

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

CyRFP1

Expression

Mammalian

Mutation

H61M (compared with CyOFP)

Promoter

CMV

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SuperNovaGreen-T2A-dTomato

Plasmid

#193798

Purpose

Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

SuperNova Green

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-GCaMP6m-Xn

Plasmid

#118976

Purpose

Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus

Depositor

Xiaodong Liu

Article

Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

Insert

GCaMP6m-XN

Expression

Mammalian

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-GLT1a

Plasmid

#162515

Purpose

Rat GLT-1a cDNA N-terminally tagged with EGFP

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GLT1a (Slc1a2 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDIV270

Plasmid

#226181

Purpose

Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

tRNA-sgRNA-tRNA

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1p (Komagataella phaffii)

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Arf1 DN T31N

Plasmid

#10833

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf1 T31N (ARF1 Human)

Tags

HA

Expression

Mammalian

Mutation

T31N. Defective in GTP binding. Dominant negati…

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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HP21-TPI-WT-no_introns

Plasmid

#131044

Purpose

Expresses TPI-Wildtype NMD reporter without introns and with a 21 nt hairpin in 5'UTR

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

TPI-Wildtype-no introns (TPI1 Human)

Expression

Mammalian

Promoter

Pcmv

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-Stuffer-HepmTurquoise2

Plasmid

#192826

Purpose

Contains stuffer sequence into which sgRNAs can be cloned and expresses mTurquoise2 from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mTurquoise2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC16A4

Plasmid

#131911

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC16A4 (SLC16A4 Human)

Expression

Mammalian

Available Since

Oct. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcmv3-IRG1-HA

Plasmid

#198181

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1)

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

ACOD1 (ACOD1 Human)

Tags

HA

Expression

Mammalian

Mutation

HA tag is added to C-terminal of target protein

Promoter

CMV

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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epi-BE4max-NG

Plasmid

#135977

Purpose

Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

Depositor

Yongming Wang

Article

Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only
GAE teo-GFP

Plasmid

#83958

Purpose

Can be used to produce inducible SIV-derived vector. Cells tranduced by lentiviral particles will overexpress GFP upon activation of the tetO promoter.

Depositor

Vincent Lotteau, Philippe Mangeot

Article

Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

Insert

TetO-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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piggyBAC-H2B mCherry-XL (R125K)

Plasmid

#189926

Purpose

Expresses nuclear-localized mCherry-XL (R125K) in mammalian cells

Depositor

Ralph Jimenez

Article

Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

Insert

mCherry-XL (R125K)

Tags

H2B

Expression

Mammalian

Mutation

differs from mCherry by R125K, W143S, I161V, Q163…

Promoter

CMV7

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 N

Plasmid

#158079

Purpose

Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 nucleocapsid (N )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4Luc-RLuc

Plasmid

#64034

Purpose

a bidirectional reporter vector allowing the study of biscistronic promoter

Depositor

Daniel Christophe

Article

Christophe-Hobertus et al Mol Cell Endocrinol. 2010 Apr 12;317(1-2

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBactin-Akna-mEGFP

Plasmid

#196866

Purpose

Expression of the centrosomal protein Akna fused to enhanced (E)-GFP

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Akna-mEGFP (Akna Mouse)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA83-GG

Plasmid

#208016

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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myc-hRAD18 dSAP

Plasmid

#68830

Purpose

Expresses c-myc-tagged human RAD18 deleting SAP domain

Depositor

Satoshi Tateishi

Article

Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

Insert

c-myc tagged human RAD18 deleting SAP domain (RAD18 Human)

Tags

c-myc

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-HaloAKAR2.2-T2A-EGFP

Plasmid

#216490

Purpose

Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.2

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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