We narrowed to 22,836 results for: Sis

1. miR-144 Trap

   Plasmid

   #60934

   Purpose

   to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoter

   Depositor

   Curt Civin

   Article

   Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

   Insert

   miR-144 Trap
   

   Use

   Lentiviral

   Tags

   GFP

   Promoter

   bidirectional phosphoglycerate kinase promoter (b…

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

2. UKNP2.2.1

   Plasmid

   #98209

   Purpose

   Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

   Depositor

   Jonathan Ball

   Article

   Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

   Insert

   HCV E1E2
   

   Expression

   Mammalian

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXIP TGBRI K to R

   Plasmid

   #19150

   Depositor

   Joan Massague

   Article

   Padua et al Cell. 2008 Apr 4. 133(1):66-77.

   Insert

   Transforming growth factor, beta receptor I (TGFBR2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K420R mutation

   Available Since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pBGC155-MXMT

   Plasmid

   #79818

   Purpose

   GFP(S65T)-based BiFC vector for plants (Positive control: MXMT)

   Depositor

   Yutaka Kodama, Masamitsu Wada

   Article

   Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

   Insert

   CaMXMT1
   

   Use

   Reporter plasmid

   Tags

   GC155/GFP (155–239)

   Expression

   Plant

   Promoter

   CaMV 35S promoter and CaMV35S

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPK1E-1+t

   Plasmid

   #48147

   Purpose

   Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-h-mmPtch1-B

   Plasmid

   #120888

   Purpose

   Expresses Ptch1-B for function assays

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Ptch1 (Ptch1 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. SIN40C.SFFV.dTomato.miR-ctrl

   Plasmid

   #169303

   Purpose

   Constitutive overexpression of miR-ctrl with dTomato as a reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   miR-ctrl
   

   Use

   Lentiviral

   Tags

   dTomato

   Promoter

   SFFV

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPR01-FnCpf1

   Plasmid

   #176529

   Purpose

   Nuclease Cpf1 adapted for Aspergillus species

   Depositor

   Kusol Pootanakit

   Article

   Abdulrachman et al BMC Biotechnol. 2021 Feb 11;21(1):15. do

   Insert

   FnCpf1
   

   Use

   CRISPR; Fungal expression

   Promoter

   TEF1

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. 2Bc-T MBP_BoMoC(ed)_I770A_6xH

   Plasmid

   #185711

   Purpose

   Recombinant B. mori truncated R2 RT expression

   Depositor

   Kathleen Collins

   Article

   Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

   Insert

   B. mori truncated R2 RT
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Changed I770 to A

   Promoter

   T7

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-SERTS585P-myc

    Plasmid

    #107498

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S585P conferring gain of function phenotype

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Expression

    Mammalian

    Mutation

    S585P

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-M2-TLR2-V660N

    Plasmid

    #12292

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    TLR2 (Tlr2 Mouse)
    

    Tags

    FLAG (FLAG-M2)

    Expression

    Mammalian

    Mutation

    M82I, H202Y and V660N; signal peptide (aa1-23) re…

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pCarCBA

    Plasmid

    #117754

    Purpose

    Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone

    Depositor

    Greg Bokinsky

    Article

    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

    Insert

    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)
    

    Expression

    Bacterial

    Promoter

    PLac(UV5)

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC-mWasabi-G4

    Plasmid

    #164168

    Purpose

    Expresses mWasabi under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mWasabi
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. SaABE8e-dimer

    Plasmid

    #138501

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSaCas9
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p5B3-T

    Plasmid

    #92021

    Purpose

    Expresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lysA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Mutation

    S219V

    Available Since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. enAsABE8e-dimer

    Plasmid

    #138507

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-dAsCas12a (En)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB427

    Plasmid

    #74821

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGWB426

    Plasmid

    #74820

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Flag-ATXN1_L588D

    Plasmid

    #48194

    Purpose

    Expresses L588D mutated Ataxin1

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Ataxin1 (ATXN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Flag-ATXN1_L588A

    Plasmid

    #48193

    Purpose

    Expresses L588A mutated Ataxin1

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Ataxin1 (ATXN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Flag-ATXN1_R665A

    Plasmid

    #48195

    Purpose

    Expresses R665A mutated Ataxin1

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Ataxin1 (ATXN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBT267_(pCA-ATG-intron-tTA2-TRE-tdT3Myc)

    Plasmid

    #36879

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    tTA2
    tdT-3Myc
    

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    insertion of a beta-globin intron with a loxP sit…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZ_lacO-cP-AOX1-eGFP

    Plasmid

    #126720

    Purpose

    yeast plasmid overexpressing eGFP

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    egfp
    

    Tags

    6xHis,Myc

    Expression

    Yeast

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBabe-Neuroserpin

    Plasmid

    #58259

    Purpose

    Retroviral vector for expressing Neuroserpin in mammalian cells

    Depositor

    Joan Massague

    Article

    Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

    Insert

    Neuroserpin (SERPINI1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

25. UKNP2.5.1

    Plasmid

    #98213

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJMP1344

    Plasmid

    #119273

    Purpose

    knockdown folA in E. aerogenes

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA folA (E. aerogenes)
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJMP1274

    Plasmid

    #119266

    Purpose

    B. subtilis deltaTn7 site and flanking sequence cloned into pACYC

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    B. subtilis deltaTn7
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJMP1273

    Plasmid

    #119265

    Purpose

    E. coli attTn7 site with B. subtilis flanking sequence cloned into pACYC

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    E. coli attTn7
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJMP1263

    Plasmid

    #119264

    Purpose

    B. subtilis attTn7 site and flanking sequence cloned into pACYC

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    B. subtilis attTn7
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJMP1106

    Plasmid

    #119249

    Purpose

    construction intermediate

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Pmyo-3::tag:SL1(G20A)

    Plasmid

    #81114

    Purpose

    Expression of exogenous SL1(G20A) in C. elegans muscle

    Depositor

    Xiao Liu

    Article

    Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

    Insert

    sls-1.2 (sls-1.2 Nematode)
    

    Tags

    illumina-adapter

    Expression

    Worm

    Mutation

    G20A

    Promoter

    promoter of myo-3

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDUAL-Pnmt1-Ost4-GFP-MTn

    Plasmid

    #154369

    Purpose

    Yeast expression of Ost4

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Ost4 (ost4 Fission Yeast)
    

    Expression

    Yeast

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p158

    Plasmid

    #62617

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCAG-WIPI2d10-364delta263-295-TEV-STREP

    Plasmid

    #171830

    Purpose

    Mammalian expression of human WIPI2d 10-364 delta263-295 with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    10-364delta263-295

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. FLAG HDAC5 S498A

    Plasmid

    #32215

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser498Ala

    Promoter

    CMV

    Available Since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pcDNA3-HA-REDD1 P139A (#625)

    Plasmid

    #99945

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    P139A

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-HA-REDD1 S137A (#627)

    Plasmid

    #99943

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S137A

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-M2-TLR2-P681H

    Plasmid

    #12290

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    TLR2 (Tlr2 Mouse)
    

    Tags

    FLAG (FLAG-M2)

    Expression

    Mammalian

    Mutation

    A74V, M82I, H202Y and P681H; signal peptide (aa1-…

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

39. pHH0103_NCK1-3/3

    Plasmid

    #91476

    Purpose

    Protein expression and purification of human SH3 domain construct NCK1-3/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NCK1-3/3 (NCK1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CMV-Cx35-Halo-C-in

    Plasmid

    #71511

    Purpose

    Mammalian expression plasmid encoding perch Cx35 with an internal HaloTag between amino acids 285 and 286 in the C-terminus.

    Depositor

    John O'Brien

    Article

    Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97.

    Insert

    Connexin 35
    

    Tags

    HaloTag (internal to sequence)

    Expression

    Mammalian

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge