We narrowed to 10,930 results for: Kars;
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Plasmid#213172PurposeCIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and Gas5 guide RNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-Calm3-U6-Gas5 gRNA
UseLentiviralTagsGFPExpressionMammalianMutationPromoterSyn1, U6Available sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPC1000
Plasmid#174829PurposeLentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit siteDepositorInsertsCRISPRi SpCas9 sgRNA cassette
PuroR-P2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationDetailed in manuscriptPromoterEF1a and mouse U6Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep
Plasmid#176534PurposeBacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrepDepositorInsertCRISPR-associated IscB (Delaware Bay metagenome)
UseTags14xHis-MBP-bdSUMO and TwinStrepExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLU-CMV-Flag-hATG5 WT
Plasmid#111803PurposeMammalian Expression of hATG5DepositorInserthATG5 (ATG5 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 S_nostop
Plasmid#152988PurposeGateway-compatible Entry vector, with insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX PLCg1(NC)-SH2
Plasmid#46471DepositorInsertPLCg1(NC) (PLCG1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 542-759PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb2-SH2
Plasmid#46440DepositorInsertGrb2 (GRB2 Human)
UseTagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 58-159PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLX304-YAP1 _60-89
Plasmid#59144PurposeYAP1 mutants that harbored a deletion of amino acide at 60-89 position, which disrupts binding to TEAD transcription factorsDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationaa60-89 deletedPromoterCMVAvailable sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTH733-2µ-RLuc/maxCFLuc
Plasmid#40608DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Ma-sfGFP 150TAG
Plasmid#197568PurposeExpression of sfGFP-150TAG with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP 150TAG
M. alvus Pyl-tRNA (6) (4x copies)
UseTagsV5-His6ExpressionMammalianMutationN150TAGPromoterCMV and U6/H1Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF4_P2A_Hygro_Barcode
Plasmid#120455PurposeBarcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertKLF4 (KLF4 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-STAT4-P2A-mCherry
Plasmid#203853PurposeTet-inducible lentiviral plasmid expressing STAT4-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertSTAT4 (STAT4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pInducer-TAZ
Plasmid#213587PurposeDoxycycline inducible expression of TAZ wild type cDNA in mammalian cellsDepositorInsertTAZ (WWTR1 Human)
UseLentiviralTags2xHAExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304-YAP1 _PDZ
Plasmid#59147PurposeYAP1 mutants that harbored a deletion of the 5 amino acidsDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMammalianMutationPDZ binding domain (last 5aa) deletedPromoterCMVAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
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ZMYND8-dTAG
Plasmid#190617PurposeLentiviral expression plasmid of human ZMYND8 fused with FKBP12G36V for dTAG-mediated degradationDepositorInsertZMYND8 (ZMYND8 Human)
UseLentiviralTagsFKBP12_G36V and Flag, 2xHAExpressionMammalianMutationsynonymous mutation for sgRNA resistance at aa214…PromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIIα-μDpTT
Plasmid#182327PurposePKA RIIα subunit-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertRIIalpha-μDronpa-TagRFP-T (PRKAR2A Human)
UseTagsFull-length human PKA type 2alpha regulatory subu…ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-STK33
Plasmid#116797PurposeLentiviral expression of STK33DepositorInsertSTK33 (STK33 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -