We narrowed to 15,923 results for: nans

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pAAV-Ef1a-DIO-mCherry-WPRE-pA

Plasmid

#37083

Purpose

Cre-dependent expression of mCherry

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

mCherry

Use

AAV and Cre/Lox; Cre-on

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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HA-Cdk9

Plasmid

#28102

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Tags

3x HA

Expression

Bacterial and Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

Plasmid

#55220

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6 and MBP, Asn10

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

Plasmid

#222991

Purpose

Bacterial expression of iGeoCas9(C2) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Promoter

tac

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b PKA Cat

Plasmid

#14921

Depositor

Susan Taylor

Article

Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

Insert

PKA catalytic subunit alpha (Prkaca Mouse)

Tags

6xHis and Thrombin cleavage site

Expression

Bacterial

Available Since

July 24, 2007

Availability

Academic Institutions and Nonprofits only

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pET28a_T5-ARG1

Plasmid

#106476

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Use

Synthetic Biology

Expression

Bacterial

Promoter

T5

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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μ2-HA-WT

Plasmid

#32752

Depositor

Alexander Sorkin

Article

Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

Insert

μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pTagRFP657-C1

Plasmid

#31872

Purpose

For fusion of TagRFP657 to N-term of insert. SEE DEPOSITOR COMMENTS BELOW.

Depositor

Vladislav Verkhusha

Article

Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

Insert

TagRFP657

Expression

Mammalian

Mutation

M44Q, K69H, F84W, S148H, S165T, D166A, M167L, L18…

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p

Plasmid

#202484

Purpose

Expresses sulfhydryl oxidase (Erv1p) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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1.3xWT HBV-Luciferase

Plasmid

#61549

Purpose

The 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of Core

Depositor

Yosef Shaul

Article

Doitsh et al Mol Cell Biol. 2004 Feb;24(4):1799-808.

Insert

1.3 times of the HBV genome with luciferase within the Core ORF

Use

Luciferase

Expression

Mammalian

Mutation

Substitution of HBV sequences with luciferase ORF…

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDUET-1_VNp-anti-6xHis mAb

Plasmid

#214952

Purpose

Bacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.

Depositor

Dan Mulvihill

Article

Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

Inserts

VNp-anti-6xHis mAb heavy chain
VNp-anti-6xHis mAb light chain

Tags

VNp6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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His-GFP-Rim2

Plasmid

#170869

Purpose

Mammalian expression of His-GFP-Rim2.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Regulating synaptic membrane exocytosis protein 2 (Rim 2) (Rims2 Rat)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS_VP35_MARV

Plasmid

#135471

Purpose

Encodes Marburg virus VP35 gene under control of the AG promoter

Depositor

Elke Mühlberger

Article

Schmidt et al J Infect Dis. 2011 Nov;204 Suppl 3:S861-

Insert

VP35

Expression

Mammalian

Mutation

None

Promoter

AG

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSPdH45

Plasmid

#199767

Purpose

Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSPdH45

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMSP1E3D1_D73C

Plasmid

#39328

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E3D1_D73C (APOA1 synthetic gene)

Tags

6xHis

Expression

Bacterial

Mutation

cys mutant of MSP1E3D1

Promoter

T7

Available Since

Sept. 11, 2012

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.GFP.miR-218-2-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85881

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-218-2-3p Sensor (MIR218-2 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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SBE-NLS-mCherry PGK-rtTA3

Plasmid

#195332

Purpose

Lentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Inserts

NLS-mCherry
rtTA3

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hPGK and miniCMV

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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His-GFP-Rab27a

Plasmid

#170861

Purpose

Mammalian expression of His-GFP-Rab27a.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rab-27a (RAB27A Human)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBMN-dGBP1-TagBFP

Plasmid

#80087

Purpose

MMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection marker

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

dGBP1-TagBFP

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Akaluc-N1

Plasmid

#120371

Purpose

Akaluc luciferase mammalian expression in -N1 backbone

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

Akaluc (human optimized)

Expression

Mammalian

Mutation

Human codon optimized

Promoter

CMV

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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