We narrowed to 9,389 results for: Coli

1. pRAR017-L31-sfGFP

   Plasmid

   #185024

   Purpose

   400 nt l31 promoter region, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31-sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Only first 90 nt of rpmE coding present

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMS22

   Plasmid

   #129710

   Purpose

   Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   Escherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)
   

   Use

   Tags

   10-Histidine tag cleavable with PreScission Prote…

   Expression

   Bacterial

   Mutation

   RssB E134G:E135S:E136G:E137S

   Promoter

   T7

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTRKH2

   Plasmid

   #71312

   Purpose

   Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

   Depositor

   Todd Klaenhammer, Rodolphe Barrangou

   Article

   O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

   Type

   Empty backbone

   Use

   E. coli-gram positive bacteria shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNK045p

   Plasmid

   #156335

   Purpose

   Expresses heterologous rRNA

   Depositor

   Ahmed Badran

   Article

   Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

   Insert

   rRNA operon, orthogonal-antiRBS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   WT antiRBS engineered to orthogonal antiRBS, PS e…

   Promoter

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD24-sfGFPx1

   Plasmid

   #51558

   Purpose

   Superfolder GFP ORF cloned into pBAD24 for expression in E. coli

   Depositor

   Francisco Malagon, Sankar Adhya

   Article

   Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

   Insert

   superfolder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSM019

   Plasmid

   #128291

   Purpose

   asymmetric plasmid partitioning E. coli - regulatory plasmid

   Depositor

   Matthew Bennett

   Article

   Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

   Insert

   Para - lp-sfyfp-parB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RepX_pET28a(+)

   Plasmid

   #172199

   Purpose

   Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

   Depositor

   Taekjip Ha

   Article

   Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

   Insert

   RepX superhelicase
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   C18L, C43S, C167V, S400C, C612A

   Promoter

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDA303

   Plasmid

   #195199

   Purpose

   Overexpressing 6His-TetR in E. coli.

   Depositor

   Rahul Sarpeshkar

   Article

   Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

   Insert

   TetR
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCas1+2

   Plasmid

   #72676

   Purpose

   Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

   Depositor

   Udi Qimron

   Article

   Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

   Insert

   Cas1, Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMA2775

    Plasmid

    #46884

    Purpose

    Lentiviral vector encoding Tet-inducible mitochondrially targeted myc-tagged E.coli exonuclease III

    Depositor

    Mikhail Alexeyev

    Article

    Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

    Insert

    exonucleaseIII E. coli
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-Tight

    Available since

    Aug. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-Ec.coaA

    Plasmid

    #50386

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Erick Strauss

    Article

    Brand et al J Biol Chem. 2005 May 27;280(21):20185-8

    Insert

    PanK
    

    Use

    Tags

    6XHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMUT2-pFF-41.5-mCherry

    Plasmid

    #192863

    Purpose

    pMUT2 based vector for sensing oxygen in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    Oxygen inducible promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMUT2-pCadBA-mCherry

    Plasmid

    #192865

    Purpose

    pMUT2 based vector for sensing pH in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    pH sensitive promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDJSVT87

    Plasmid

    #109302

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Use

    Tags

    OmpA 1-27, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBRα

    Plasmid

    #53731

    Purpose

    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    αNTD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLRD16

    Plasmid

    #208268

    Purpose

    Production of syn-stemar-13-ene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    osCPS4
    osKSL8
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLRD13

    Plasmid

    #208265

    Purpose

    Production of ent-cassa-12,15-diene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    ptmT2
    osKSL7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLRD05

    Plasmid

    #208259

    Purpose

    Production of isopimara-8,15-diene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    cldB
    sare_1287
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET24c(+)-EFTs

    Plasmid

    #31173

    Depositor

    Linda Spremulli

    Article

    Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

    Insert

    E. coli elongation factor Ts
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pMUT2-pLac-mCherry

    Plasmid

    #192859

    Purpose

    pMUT2 based vector for sensing lactic acid in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    lldR operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28b-6His-AcpS

    Plasmid

    #145376

    Purpose

    Expression of E. coli AcpS

    Depositor

    Robert Tomko

    Article

    Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

    Insert

    AcpS (acpP E. coli)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. Ec2Avs2

    Plasmid

    #188790

    Purpose

    Avs2 from Escherichia coli NCTC10650

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    Ec2Avs2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTJV1ScKm-rpoB

    Plasmid

    #166980

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    NeoR/KanR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAA3

    Plasmid

    #128280

    Purpose

    asymmetric plasmid partitioning E. coli - target plasmid

    Depositor

    Matthew Bennett

    Article

    Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

    Insert

    Piq - mRFP, parS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCC28

    Plasmid

    #128284

    Purpose

    asymmetric plasmid partitioning E. coli - target plasmid

    Depositor

    Matthew Bennett

    Article

    Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

    Insert

    Piq - mRFP, sopC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET22b-ecDHFR

    Plasmid

    #109055

    Purpose

    Bacterial expression of E.coli DHFR protein

    Depositor

    Stephen Benkovic

    Article

    Cameron et al Biochemistry. 1997 Dec 16;36(50):15792-8

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EcAvs4

    Plasmid

    #188794

    Purpose

    Avs4 from Escherichia coli NCTC11132

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    EcAvs4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSM040

    Plasmid

    #128294

    Purpose

    asymmetric plasmid partitioning E. coli - target plasmid

    Depositor

    Matthew Bennett

    Article

    Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

    Insert

    Piq - mRFP, parS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDJSVT91

    Plasmid

    #109305

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Use

    Tags

    SufI 1-30, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSpal2At

    Plasmid

    #78286

    Purpose

    Expresses A. thaliana PAL2 in E. coli

    Depositor

    David Nielsen

    Article

    McKenna et al Metab Eng. 2011 Sep;13(5):544-54. doi: 1

    Insert

    PAL2 (PAL2 Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    *see below

    Promoter

    lac

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSM015

    Plasmid

    #128289

    Purpose

    asymmetric plasmid partitioning E. coli - regulatory plasmid

    Depositor

    Matthew Bennett

    Article

    Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

    Insert

    Para - lp-cfp-parB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDB88

    Plasmid

    #26958

    Depositor

    Michael Glickman

    Article

    Barkan et al Gene. 2011 Jan 1;470(1-2):31-6. doi: 10.

    Insert

    e. coli galactokinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    cloned under the MOP promoter

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDJSVT89

    Plasmid

    #109303

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Use

    Tags

    TamA 1-27, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDJSVT90

    Plasmid

    #109304

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Use

    Tags

    TamA 1-27, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a-soNadV

    Plasmid

    #83363

    Purpose

    Expresses nadV from Shewanella oneidensis in Escherichia coli

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

    Insert

    nadV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSVD5

    Plasmid

    #18877

    Depositor

    Jay Keasling

    Article

    Van Dien et al Appl Environ Microbiol. 1997 May . 63(5)

    Insert

    exopolyphosphatase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2008

    Availability

    Academic Institutions and Nonprofits only

37. pAW204

    Plasmid

    #113244

    Purpose

    E. coli expression vector for IHFa F13A + IHFb

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    IHFa F13A (ihfA E. coli)
    IHFb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F13A

    Promoter

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMUT2-pAto-mCherry

    Plasmid

    #192858

    Purpose

    pMUT2 based vector for sensing acetoacetate in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    Acetoacetate inducible promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAA6

    Plasmid

    #128282

    Purpose

    asymmetric plasmid partitioning E. coli - target plasmid

    Depositor

    Matthew Bennett

    Article

    Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

    Insert

    Piq- mRFP, parS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSPB866

    Plasmid

    #128304

    Purpose

    asymmetric plasmid partitioning E. coli - regulatory plasmid

    Depositor

    Matthew Bennett

    Article

    Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

    Insert

    Para - GCN4zip.MSE-cfp-parB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge