We narrowed to 11,061 results for: AGA
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Plasmid#208979PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Synthetic, Human)
UseCRISPRExpressionMammalianPromoterCMV and U6 in tandemAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Gollum Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2
Plasmid#210747PurposeCoding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 3DepositorInsertsSaSp Cas9
Gollum sgRNA GFP Target 3
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationN-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCX:γB2ΔICD-EC1-G4S-mTQ2ΔC6
Plasmid#209089PurposemTurquoise2-inserted PcdhgB2 lacking an intracellular domain (ICD)DepositorAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
XPO6-2Strep
Plasmid#210356PurposeExpresses XPO6 fused with 2Strep in mammalian cellsDepositorAvailable SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRAM2-2Strep
Plasmid#210374PurposeExpresses TRAM2 fused with 2Strep in mammalian cellsDepositorAvailable SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRAM1-2Strep
Plasmid#210352PurposeExpresses TRAM1 fused with 2Strep in mammalian cellsDepositorAvailable SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
SGTA-2Strep
Plasmid#210353PurposeExpresses SGTA fused with 2Strep in mammalian cellsDepositorAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:cIPAD-3 (ΔICD)
Plasmid#208518PurposeddGFP-A and ddGFP-B3-inserted Pcdhα4 lacking an intracellular domain (ICD)DepositorAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:cIPAD-1 (ΔICD)
Plasmid#208517PurposeddGFP-A and ddGFP-B1-inserted Pcdhα4 lacking an intracellular domain (ICD)DepositorAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:B3-α4ΔICD
Plasmid#208513PurposeddGFP-B3-inserted Pcdhα4 lacking an intracellular domain (ICD)DepositorAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:B1-α4ΔICD
Plasmid#208511PurposeddGFP-B1-inserted Pcdhα4 lacking an intracellular domain (ICD)DepositorAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:α4ΔICD-EC4-GA
Plasmid#208512PurposeddGFP-A-inserted Pcdhα4 lacking an intracellular domain (ICD)DepositorAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- HTA1prom-TIR1-FLAG
Plasmid#189725PurposeEnables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)DepositorInsertOsTIR1
TagsFLAGExpressionYeastPromoterHTA1Available SinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- TEF2prom-TIR1-FLAG
Plasmid#189726PurposeEnables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)DepositorInsertOsTIR1
TagsFLAGExpressionYeastPromoterTEF2Available SinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain
Plasmid#183387PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertredesigned Caspase-4 (AA94-377) (CASP4 Human)
TagsHis-TEVExpressionBacterialMutationANR261ENP, D315H, M318K, T352KPromoterT7Available SinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-mouse-Caspase-1-H340D
Plasmid#183362PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorAvailable SinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-dog-Caspase-1/4a-triple mutant-H342D_K345M_K379T
Plasmid#183364PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1/4a (CASP4 Canis lupus familiaris)
UseRetroviralTagsMycMutationH342D_K345M_K379TPromoterMESVAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-dog-Caspase-1/4b
Plasmid#183365PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-dog-Caspase-1/4a
Plasmid#183363PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-Cas9
Plasmid#120352PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonExpressionMammalianPromotertet-on and CBhAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-KRAB-dCas9
Plasmid#120355PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonExpressionMammalianPromotertet-on and CBhAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-sgRNA-M7-IRES-CFP
Plasmid#114730PurposesgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc geneDepositorInsertsgRNA M7
UseCRISPRExpressionMammalianAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
CeNL(Ca2+)_110μ-pcDNA3
Plasmid#111929PurposeCyan color bright luminescent calcium indicator with low affinityDepositorInsertCeNL(Ca2+)_110μ
ExpressionMammalianMutationE31D, F92W, E104D. D133E at CaMPromoterCMVAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G11-R0
Plasmid#101805PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyPromoterSpy 1049Available SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-SsoPCNA3
Plasmid#107069PurposeExpresses S. solfataricus PCNA3 fused to MBP in E. coliDepositorInsertS. solfataricus PCNA3
Tags6xHis-thrombin cleavage site and MBPExpressionBacterialPromotertac promoterAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
LEP-shTADA1.1580
Plasmid#105568Purposeretrovirally express TADA1 shRNA with puro resistance and GFP markerDepositorInsertTADA1 shRNA
UseRNAi and RetroviralExpressionMammalianPromoterMSCV-LTRAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
LEP-shTAF2.1635
Plasmid#105561Purposeretrovirally express TAF2 shRNA with puro resistance and GFP markerDepositorInsertTAF2 shRNA
UseRNAi and RetroviralExpressionMammalianPromoterMSCV-LTRAvailable SinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G14-R0
Plasmid#101806PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyPromoterSpy 1049Available SinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G10-R0
Plasmid#101804PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyPromoterSpy 1049Available SinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD1R
Plasmid#86969PurposeExpresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coliDepositorInsertPCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
TagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDHPromoterT7 promoterAvailable SinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-016
Plasmid#78704PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-015
Plasmid#78703PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-010
Plasmid#78697PurposeA strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-008
Plasmid#78695PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-007
Plasmid#78694PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
shCDK4(1)
Plasmid#73550PurposeshRNA(1) against CDK4DepositorInsertshRNA against CDK4
UseRetroviralExpressionMammalianPromoterLTRAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST attB BmOr2 (Y464A)
Plasmid#73927PurposeUAS induced expression of Bombyx mori odorant receptor 2 (OR2) cDNA with 464 amino acid change of Tyrosine to AlanineDepositorInsertBmOr2 (Y464A)
ExpressionInsectMutationchanged 464 Tyrosine to AlaninePromoterhsp70Available SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-puro-U6-sgRNA-TS4
Plasmid#69236PurposeU6 driven SpCas9 sgRNA expression for TS4 siteDepositorInsertTS4 sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL671
Plasmid#62825PurposeLim Lab plasmid: AmpR + PCon::glgC::gfp::T1T2 terminator + ColE1DepositorInsertgfp
UseSynthetic BiologyTagsglgC leaderExpressionBacterialPromoterpConAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1017
Plasmid#62828PurposeLim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + ColE1DepositorInsertgfp
UseSynthetic BiologyTagsm8::glgC leaderExpressionBacterialPromoterpConNoHindAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119
Plasmid#66136PurposeExpresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
TagsSulfolobus solfataricus PCNA3ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119(T214V)
Plasmid#66137PurposeExpresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
TagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationT214VPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 T180C
Plasmid#66181PurposeExpresses the T180C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
TagsHis6 tagExpressionBacterialMutationT180CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 R112C
Plasmid#66180PurposeExpresses the R112C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
TagsHis6 tagExpressionBacterialMutationR112CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 E105C
Plasmid#66176PurposeExpresses the E105C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
TagsHis6 tagExpressionBacterialMutationE105CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119(D77R/T214V)
Plasmid#66138PurposeExpresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
TagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationD77R, T214VPromoterT7 promoterAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA5woH
Plasmid#65019PurposeExpresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureusDepositorInsertsortase A
TagsS tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, D160N, D165A, K19…Promotertetracycline promoter/operatorAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-KQ
Plasmid#64982PurposeExpresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108QAvailable SinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_63/pRSETB
Plasmid#51980Purposebacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nMDepositorInsertNano-lantern_CaM_E104Q-4GS
Tags6xHisExpressionBacterialMutationS257G in Rluc8Available SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pattB-3xFlag-Loqs-PA
Plasmid#41108DepositorAvailable SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only