-
Plasmid#40728PurposeModified pcDNA3.1 vector for ligation independent cloning, including a ccdB cassette for eliminating no-insert backgroundDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only
-
pUAST-3x attB
Plasmid#220329Purposeincludes 3 standard UAS cassettesDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-mTagBFP2 PuroR
Plasmid#101893PurposeLentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmTagBFP2ExpressionMutationPromoterCMV immediate earlyAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-mRuby2 PuroR
Plasmid#84386PurposeLentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmRuby2ExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLfa[Tc'hsp5'3'UTR]fa
Plasmid#86450PurposeHeat shock-inducible promoter including hsp-UTRs, ubiquitous expression upon heat-shock of any inserted gene of interest in Tribolium; (Shuttle plasmid)DepositorInsertTc'hsp5'3'UTR
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET14b_tev_PDZ1
Plasmid#59739Purposeexpresses PDZ1 domain of human ZO-1 in bacteriaDepositorInsertzonula occludens-1 (TJP1 Human)
UseTagsHIS tag and thrombin clvgExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCI-SMN2-skipping-GLuc
Plasmid#218670PurposeAlternative splicing signaling: signal decrease with Ex7 inclusion increasingDepositorInsertSMN2 (SMN2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKP1469
Plasmid#160815PurposeExpression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD)DepositorInsertexbB
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-mTagBFP2 L1-R5
Plasmid#84388PurposeMultisite Gateway entry vector for expression of mTagBFP2-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsmTagBFP2ExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
2xUAS-1xhsp70-attB
Plasmid#220317Purposeincludes 2 UAS cassettes and 1 heat-shock inducible hsp70 casetteDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-mCardinal L1-R5
Plasmid#84392PurposeMultisite Gateway entry vector for expression of mCardinal-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsmCardinalExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK531
Plasmid#72512PurposeProduces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)DepositorInserthypothetical protein in purSQL operon
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDUAL OCLN (GFP)
Plasmid#86982PurposeLentiviral expression construct encoding Occludin and GFP from separate promotersDepositorInsertOCLN (OCLN Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFV/hPGKAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Hist4HG-N-18
Plasmid#62757PurposeLocalization: Histones, N1 Cloning Vector, Excitation:487, Emission:509DepositorInsertHist4HG (HIST1H4G Human)
UseTagsmEmeraldExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJK546
Plasmid#72518PurposeProduces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)DepositorInserthypothetical protein in purSQL operon
UseTagsExpressionBacterialMutationcontains additional Ala2PromoterT7Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK567
Plasmid#72522PurposeProduces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)DepositorInserthypothetical protein in purSQL operon
UseTagsExpressionBacterialMutationcontains additional Ala2PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
mP_ILB_Vector 1
Plasmid#232193PurposeThe plasmid vector designed for the expression of four genes in oleaginous yeasts, including Yarrowia lipolytica and Rhodotorula toruloides.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 3, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBABEneo mt T Ag
Plasmid#133713PurposeLarge T antigen mutant form in pBABEneo vector for mammalian expression.DepositorInsertSV40gp6 (SV40gp6 SV40)
UseRetroviralTagsExpressionMutationaa 1-354 of T antigen (including aa 1-130); no p5…PromoterAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK530
Plasmid#72511PurposeProduces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster with C-terminal His6 tag (AaOrfYH6)DepositorInserthypothetical protein in purSQL operon
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGR595
Plasmid#213785PurposeIntegrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.DepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)
Plasmid#51656Purposehsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrangeDepositorInsertQuasar2-mOrange-Chr64-GFP
UseLentiviralTagsGFP and mOrangeExpressionMammalianMutationPromoterhuman synapsinAvailable sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNHA_OROV_NCP
Plasmid#208937PurposeExpresses N-terminally HA-tagged Oropouche orthobunyavirus nucleocapsid protein from CMV promoter in mammalian cellsDepositorInsertNucleocapsid
UseTagsHAExpressionMammalianMutationDeleted start codon so HA included at N-terminusPromoterCMVAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTipi2.1-AZU_SP
Plasmid#229144PurposeAllows for expression of recombinant antibodies. Includes human azurocidin signal peptide.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGR596
Plasmid#213787PurposeIntegrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationSee manuscriptPromoterAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGR618
Plasmid#213786PurposeIntegrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBB-Tv TP-DNAP1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-mitfa2.1
Plasmid#81234Purposezebrafish mitfa promoter entry vector for multisite Gateway cloningDepositorInsertmitfa promoter (mitfa Zebrafish)
UseMultisite gateway entry vectorTagsExpressionMutationPromotermitfa, 2.1kb including 5' UTRAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFB-CT10HF-LIC
Plasmid#39191PurposeSGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selectionDepositorTypeEmpty backboneUseBaculovirusTagsTEV-His-FLAGExpressionMutationPromoterpolyhedrinAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1Q1
Plasmid#97326PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1Q1
UseTags6xHis - TEVExpressionBacterialMutationAddition of Q residue after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKC1620-SUP35wt
Plasmid#29730DepositorInsertSUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationWild type gene including genomic sequence 955 bp …PromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNHA_OROV_NSs
Plasmid#208934PurposeExpresses N-terminally HA-tagged Oropouche orthobunyavirus NSs protein from CMV promoter in mammalian cellsDepositorInsertNSs
UseTagsHAExpressionMammalianMutationDeleted 69 bp at 5' end (including ATG) and …PromoterCMVAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X1
Plasmid#59200PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertkrt1-ex1
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-RLuc
Plasmid#83282Purposerecombinant AAV vector packaging single-stranded Renilla Luciferase under the CAG promoter (includes WPRE element)DepositorInsertRenilla Luciferase
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
M3-PAmcherry1
Plasmid#114189Purposestudying clustering of M3 receptors by superresolution microscopy (PALM)DepositorInsertMuscarin acetylcholine receptor 3 (CHRM3 Human)
UseTags3xHA and PAmCherryExpressionMammalianMutationPromoterSV40Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHelper_TS_V2_ampR
Plasmid#214467PurposeHelper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.DepositorArticleInsertCspRecT / Bxb-1
UseSynthetic BiologyTagsExpressionMutationPromoterXylS / AraCAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC301
Plasmid#31851DepositorInsertGFP (including ATG start codon)
UseTagsExpressionBacterialMutationPromoterSODAvailable sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH671-SUP35-H348Q
Plasmid#29732DepositorInsertSUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationH348Q mutant gene of SUP35 including genomic sequ…PromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJT118
Plasmid#31395DepositorInsertpJT118
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-IGR13
Plasmid#59282PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.DepositorInsert17kb upstream krt5
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X39
Plasmid#59263PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertKrt83-ex5-7
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X33
Plasmid#59257PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertKrt81-ex4-7
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X14
Plasmid#59238PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertKrt8-ex5-8
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceDec. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-IGR12
Plasmid#59281PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.DepositorInsert1.5kb upstream krt5
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJT116
Plasmid#31394DepositorInsertcca cluster from Synechocystis PCC6803
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28-Flag-RBM24-1~93
Plasmid#208633Purposefor E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93DepositorInserthuman RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
UseTags6xHis and FlagExpressionBacterialMutationonly aa 1~93, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK576
Plasmid#72527PurposeEncodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein associated with purSyQL cluster (AaOrfY)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon
UseGene synthesisTagsExpressionMutationsynthetic genePromotern/aAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X16
Plasmid#59240PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertkrt71-ex7
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceDec. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X10
Plasmid#59234PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertKrt6b-ex5-9
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceDec. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X42
Plasmid#59266PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertKrt84-ex9
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceDec. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X37
Plasmid#59261PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertKrt82-ex7-8
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceDec. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-INT1
Plasmid#59286PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intronic region of the keratin type II cluster.DepositorInsertKrt7-Intron 2
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceDec. 3, 2014AvailabilityAcademic Institutions and Nonprofits only