Skip to main content
Addgene
Showing: 1351 - 1400 of 2318 results
  1. pUB2.1

    Plasmid
    #40728
    Purpose
    Modified pcDNA3.1 vector for ligation independent cloning, including a ccdB cassette for eliminating no-insert background
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
  2. pUAST-3x attB

    Plasmid
    #220329
    Purpose
    includes 3 standard UAS cassettes
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pLenti Lifeact-mTagBFP2 PuroR

    Plasmid
    #101893
    Purpose
    Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mTagBFP2
    Expression
    Mutation
    Promoter
    CMV immediate early
    Available since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pLenti Lifeact-mRuby2 PuroR

    Plasmid
    #84386
    Purpose
    Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mRuby2
    Expression
    Mutation
    Promoter
    CMV immediate early
    Available since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. pSLfa[Tc'hsp5'3'UTR]fa

    Plasmid
    #86450
    Purpose
    Heat shock-inducible promoter including hsp-UTRs, ubiquitous expression upon heat-shock of any inserted gene of interest in Tribolium; (Shuttle plasmid)
    Depositor
    Insert
    Tc'hsp5'3'UTR
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pET14b_tev_PDZ1

    Plasmid
    #59739
    Purpose
    expresses PDZ1 domain of human ZO-1 in bacteria
    Depositor
    Insert
    zonula occludens-1 (TJP1 Human)
    Use
    Tags
    HIS tag and thrombin clvg
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. pCI-SMN2-skipping-GLuc

    Plasmid
    #218670
    Purpose
    Alternative splicing signaling: signal decrease with Ex7 inclusion increasing
    Depositor
    Insert
    SMN2 (SMN2 )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pKP1469

    Plasmid
    #160815
    Purpose
    Expression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD)
    Depositor
    Insert
    exbB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pENTR CMVie-Lifeact-mTagBFP2 L1-R5

    Plasmid
    #84388
    Purpose
    Multisite Gateway entry vector for expression of mTagBFP2-tagged Lifeact in cells including neurons
    Depositor
    Insert
    Lifeact
    Use
    Gateway
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV immediate early
    Available since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  10. 2xUAS-1xhsp70-attB

    Plasmid
    #220317
    Purpose
    includes 2 UAS cassettes and 1 heat-shock inducible hsp70 casette
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. pENTR CMVie-Lifeact-mCardinal L1-R5

    Plasmid
    #84392
    Purpose
    Multisite Gateway entry vector for expression of mCardinal-tagged Lifeact in cells including neurons
    Depositor
    Insert
    Lifeact
    Use
    Gateway
    Tags
    mCardinal
    Expression
    Mammalian
    Mutation
    Promoter
    CMV immediate early
    Available since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  12. pJK531

    Plasmid
    #72512
    Purpose
    Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)
    Depositor
    Insert
    hypothetical protein in purSQL operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
  13. pDUAL OCLN (GFP)

    Plasmid
    #86982
    Purpose
    Lentiviral expression construct encoding Occludin and GFP from separate promoters
    Depositor
    Insert
    OCLN (OCLN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV/hPGK
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
  14. mEmerald-Hist4HG-N-18

    Plasmid
    #62757
    Purpose
    Localization: Histones, N1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Insert
    Hist4HG (HIST1H4G Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
  15. pJK546

    Plasmid
    #72518
    Purpose
    Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)
    Depositor
    Insert
    hypothetical protein in purSQL operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    contains additional Ala2
    Promoter
    T7
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
  16. pJK567

    Plasmid
    #72522
    Purpose
    Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)
    Depositor
    Insert
    hypothetical protein in purSQL operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    contains additional Ala2
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
  17. mP_ILB_Vector 1

    Plasmid
    #232193
    Purpose
    The plasmid vector designed for the expression of four genes in oleaginous yeasts, including Yarrowia lipolytica and Rhodotorula toruloides.
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 3, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  18. pBABEneo mt T Ag

    Plasmid
    #133713
    Purpose
    Large T antigen mutant form in pBABEneo vector for mammalian expression.
    Depositor
    Insert
    SV40gp6 (SV40gp6 SV40)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    aa 1-354 of T antigen (including aa 1-130); no p5…
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
  19. pJK530

    Plasmid
    #72511
    Purpose
    Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster with C-terminal His6 tag (AaOrfYH6)
    Depositor
    Insert
    hypothetical protein in purSQL operon
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
  20. pGR595

    Plasmid
    #213785
    Purpose
    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.
    Depositor
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  21. Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

    Plasmid
    #51656
    Purpose
    hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange
    Depositor
    Insert
    Quasar2-mOrange-Chr64-GFP
    Use
    Lentiviral
    Tags
    GFP and mOrange
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    March 10, 2014
    Availability
    Academic Institutions and Nonprofits only
  22. pNHA_OROV_NCP

    Plasmid
    #208937
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus nucleocapsid protein from CMV promoter in mammalian cells
    Depositor
    Insert
    Nucleocapsid
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted start codon so HA included at N-terminus
    Promoter
    CMV
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  23. pTipi2.1-AZU_SP

    Plasmid
    #229144
    Purpose
    Allows for expression of recombinant antibodies. Includes human azurocidin signal peptide.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 9, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  24. pGR596

    Plasmid
    #213787
    Purpose
    Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
    Depositor
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    See manuscript
    Promoter
    Available since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  25. pGR618

    Plasmid
    #213786
    Purpose
    Integrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
    Depositor
    Insert
    BB-Tv TP-DNAP1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  26. p5E-mitfa2.1

    Plasmid
    #81234
    Purpose
    zebrafish mitfa promoter entry vector for multisite Gateway cloning
    Depositor
    Insert
    mitfa promoter (mitfa Zebrafish)
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    mitfa, 2.1kb including 5' UTR
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
  27. pFB-CT10HF-LIC

    Plasmid
    #39191
    Purpose
    SGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection
    Type
    Empty backbone
    Use
    Baculovirus
    Tags
    TEV-His-FLAG
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  28. pMSP1D1Q1

    Plasmid
    #97326
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Insert
    MSP1D1Q1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of Q residue after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
  29. UKC1620-SUP35wt

    Plasmid
    #29730
    Insert
    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Wild type gene including genomic sequence 955 bp …
    Promoter
    Available since
    Sept. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
  30. pNHA_OROV_NSs

    Plasmid
    #208934
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Insert
    NSs
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 69 bp at 5' end (including ATG) and …
    Promoter
    CMV
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  31. pLV-Enh eGFP Reporter-muKC2-X1

    Plasmid
    #59200
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Insert
    krt1-ex1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
  32. pAAV-CAG-RLuc

    Plasmid
    #83282
    Purpose
    recombinant AAV vector packaging single-stranded Renilla Luciferase under the CAG promoter (includes WPRE element)
    Depositor
    Insert
    Renilla Luciferase
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
  33. M3-PAmcherry1

    Plasmid
    #114189
    Purpose
    studying clustering of M3 receptors by superresolution microscopy (PALM)
    Insert
    Muscarin acetylcholine receptor 3 (CHRM3 Human)
    Use
    Tags
    3xHA and PAmCherry
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  34. pHelper_TS_V2_ampR

    Plasmid
    #214467
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.
    Depositor
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    XylS / AraC
    Available since
    April 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  35. pTH671-SUP35-H348Q

    Plasmid
    #29732
    Insert
    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    H348Q mutant gene of SUP35 including genomic sequ…
    Promoter
    Available since
    Sept. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
  36. pLV-Enh eGFP Reporter-muKC2-IGR13

    Plasmid
    #59282
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Insert
    17kb upstream krt5
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
  37. pLV-Enh eGFP Reporter-muKC2-X39

    Plasmid
    #59263
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Insert
    Krt83-ex5-7
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
  38. pLV-Enh eGFP Reporter-muKC2-X33

    Plasmid
    #59257
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Insert
    Krt81-ex4-7
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
  39. pLV-Enh eGFP Reporter-muKC2-X14

    Plasmid
    #59238
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Insert
    Krt8-ex5-8
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
  40. pLV-Enh eGFP Reporter-muKC2-IGR12

    Plasmid
    #59281
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Insert
    1.5kb upstream krt5
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
  41. pET28-Flag-RBM24-1~93

    Plasmid
    #208633
    Purpose
    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93
    Depositor
    Insert
    human RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
    Use
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    only aa 1~93, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  42. pJK576

    Plasmid
    #72527
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein associated with purSyQL cluster (AaOrfY)
    Depositor
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    Use
    Gene synthesis
    Tags
    Expression
    Mutation
    synthetic gene
    Promoter
    n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
  43. pLV-Enh eGFP Reporter-muKC2-X16

    Plasmid
    #59240
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Insert
    krt71-ex7
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
  44. pLV-Enh eGFP Reporter-muKC2-X10

    Plasmid
    #59234
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Insert
    Krt6b-ex5-9
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
  45. pLV-Enh eGFP Reporter-muKC2-X42

    Plasmid
    #59266
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Insert
    Krt84-ex9
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
  46. pLV-Enh eGFP Reporter-muKC2-X37

    Plasmid
    #59261
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Insert
    Krt82-ex7-8
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
  47. pLV-Enh eGFP Reporter-muKC2-INT1

    Plasmid
    #59286
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intronic region of the keratin type II cluster.
    Depositor
    Insert
    Krt7-Intron 2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1351 - 1400 of 2318 results