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Plasmid#154299PurposeA expression vector in Dictyostelium discoideum containing the Blasticidin S resistant gene and the TED module for GFP-negative screeningDepositorTypeEmpty backboneUseDictyostelium expressionTagsExpressionMutationPromoteractin15Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only
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pDYU1G-TED
Plasmid#154298PurposeA expression vector in Dictyostelium discoideum containing the G418 resistant gene and the TED module for GFP-negative screeningDepositorTypeEmpty backboneUseDictyostelium expressionTagsExpressionMutationPromoteractin15Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-HALO-3xHA
Plasmid#188937PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-HALO-3xHA
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-fSNAP-3xHA
Plasmid#188935PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-fSNAP-3xHA
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-fCLIP-3xHA
Plasmid#188936PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-fCLIP-3xHA
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQadd2
Plasmid#135662PurposeClostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R.DepositorInsertLox511/66 and LoxFas/71
UseClostridial vector, pamβ1 originTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-HALO-GS
Plasmid#188940PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertpBS-SKII-URA-GAL1-HALO-GS
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-DHFR-3xHA
Plasmid#188934PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-DHFR-3xHA
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTDpelB-NTwinStrep
Plasmid#45940Purposeuse to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequenceDepositorTypeEmpty backboneUseTags6xHis, PelB, and Twin-StrepExpressionBacterialMutationPromoterTrc (lactose/IPTG inducible)Available sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-TAF5-∆ex8
Plasmid#209048PurposeGateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8)DepositorInsertTAF5 deltaexon-8
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly_spike-in
Plasmid#125151PurposeVector to measure CP candidates (spike-in) expression by determining the abundance of transcripts originating from the candidate by a D.pse enhancer in fly cellsDepositorInsertD. pseudoobscura peak #47 enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX458-tRNA-SpCas9
Plasmid#195183PurposeVector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromotertRNA-GlnAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-TAF5-FL_WobbleMut
Plasmid#209053PurposeGateway-compatible entry vector, with insert of the full length TAF5 gene mutated to be resistant to TAF5 siRNADepositorInsertTAF5 full length
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-TAF5-∆ex8_WobbleMut
Plasmid#209054PurposeGateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNADepositorInsertTAF5 deltaexon-8
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)
Plasmid#114087PurposeU6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)DepositorInsertentry vector for FnCas12a crRNAs
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-MbCas12a crRNA-BsmBI cassette (BPK4449)
Plasmid#114088PurposeU6 promoter crRNA entry vector used for all MbCas12a crRNAs (clone spacer oligos into BsmBI cassette)DepositorInsertentry vector for MbCas12a crRNAs
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRP935
Plasmid#159302PurposeYeast tagging vector to create gene fusion with yeast codon optimized mAmetrine under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP1999
Plasmid#159308PurposeYeast tagging vector to create gene fusion with yeast codon optimized mCardinal under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP2000
Plasmid#159309PurposeYeast tagging vector to create gene fusion with yeast codon optimized mNeptune2 under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP1975
Plasmid#159310PurposeYeast tagging vector to create gene fusion with yeast codon optimized mNeongreen under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP2002
Plasmid#159311PurposeYeast tagging vector to create gene fusion with yeast codon optimized mTurquoise2 under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP266
Plasmid#159304PurposeYeast tagging vector to create gene fusion with yeast codon optimized mKate2 under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP265
Plasmid#159301PurposeYeast tagging vector to create gene fusion with yeast codon optimized mTFP1 under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP1931
Plasmid#159306PurposeYeast tagging vector to create gene fusion with yeast codon optimized mRuby2 under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-PhiYellow-SpHis5
Plasmid#44651PurposeYeast tagging vector to create gene fusion with PhiYellow with SpHis5 selectionDepositorInsertphiYellow
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-NUDT17-C-HA-Puro
Plasmid#195182PurposeLentiviral vector for DOX-inducible NUDT17 gene expression with C-terminal HA tag. Gateway expression system. Selection by puromycin.DepositorInsertNUDT17 (NUDT17 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMV promoter and minimal CMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-G614 (C-flag)
Plasmid#156421PurposeMammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorInsertSARS-CoV-2 S protein (S SARS CoV2)
UseTagsFlagExpressionMammalianMutationD614GPromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-D614 (C-flag)
Plasmid#156420PurposeMammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorInsertSARS-CoV-2 S protein (S SARS CoV2)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-D614 (NC-flag)
Plasmid#156418PurposeMammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorInsertSARS-CoV-2 S protein (S SARS CoV2)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-G614 (NC-flag)
Plasmid#156419PurposeMammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorInsertSARS-CoV-2 S protein (S SARS CoV2)
UseTagsFlagExpressionMammalianMutationD614GPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMS1097
Plasmid#29028PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle201 (SLC6A5 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-Cre-NLSExpressionMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-NUDT18-C-FLAG-Puro
Plasmid#195181PurposeLentiviral vector for DOX-inducible NUDT18 gene expression with C-terminal FLAG tag. Gateway expression system. Selection by puromycin.DepositorInsertNUDT18 (NUDT18 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterminimal CMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
FRP2008
Plasmid#159312PurposeYeast tagging vector to create gene fusion with yeast codon optimized mKOkappa under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationV2delPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-mCherry-SpHis5
Plasmid#44643PurposeYeast tagging vector to create gene fusion with mCherry with SpHis5 selectionDepositorInsertmCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-mDsRed-SpHis5
Plasmid#44639PurposeYeast tagging vector to create gene fusion with mDsRed with SpHis5 selectionDepositorInsertmDsRed
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
FRP2010
Plasmid#159313PurposeYeast tagging vector to create gene fusion with yeast codon optimized tSapphire under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationV2del, L231HPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB-LIC C
Plasmid#62288PurposeBaculovirus expression vector for secretion with N-terminal Honeybee melittin signal and C-terminal 8xHis tagDepositorTypeEmpty backboneUseBaculovirusTags8XHis tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSJ111
Plasmid#78707PurposeModular vector for constitutive secreted amylase reporter expressionDepositorInsertamyEopt
UseTagsExpressionBacterialMutationPromoterPvegAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TetON-Bmpr1A-ca
Plasmid#217536PurposeAAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptorDepositorArticleInsertconstitutively active BMP receptor 1a (BMPR1A Human)
UseAAVTagsHAExpressionMammalianMutationPromoterTet operon (TetO)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLOX-TERT-iresTK
Plasmid#12245Purpose2nd generation lentiviral vector for reversible immortalization of human primary cellsDepositorInsertTERT, HSV1-TK (TERT Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationLoxP in 3' LTR.PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gβγ-BERKY1
Plasmid#158429PurposeBRET biosensor for detection of free Gβγ in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-GRK3ct
UseLentiviralTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
Plasmid#68719PurposeCre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.DepositorHas ServiceAAV1InsertmRuby2-P2A-GCaMP6f
UseAAVTagsExpressionMutationPromoterCAG-FLEXAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαi*-BERKY3
Plasmid#158428PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseLentiviralTagsMycExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαq*-BERKY1
Plasmid#158430PurposeBRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-GRK2(RH)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαi*-BERKY1
Plasmid#158427PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-KB1753-myc
UseLentiviralTagsmycExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-eDIO-ChR2-EYFP
Plasmid#104387PurposePiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.DepositorInsertChannelrhodopsin 2
UseCre/Lox; PiggybacTagsEYFPExpressionMammalianMutationE134RPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBGO
Plasmid#62671PurposeDonor vector for mFTH1 knock-in via landing padDepositorInsertFTH1
UseCre/LoxTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-PBG-WPRE-hGH
Plasmid#74287PurposepAAV vector to express PBG (chimeric Glycoprotein) in a Cre-dependent mannerDepositorInsertPBG (chimeric rabies Glycoprotein)
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST26-MSRA-C-HA
Plasmid#195177PurposeVector for transient expression of MSRA gene with C-terminal HA tag, gene flanked by att sites for gateway cloningDepositorInsertMSRA (MSRA Human)
UseTagsHAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST26-PPP2CB-C-HA
Plasmid#195179PurposeVector for transient expression of PPP2CB gene with C-terminal HA tag, gene flanked by att sites for gateway cloningDepositorInsertPPP2CB (PPP2CB Human)
UseTagsHAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only