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Addgene

We narrowed to 1,454 results for: cre recombinase

Showing: 1351 - 1400 of 1454 results
  1. p140Tol2-Olactb:SABER

    Plasmid
    #226829
    Purpose
    Expression of beta actin driven BFP and molecular barcode array for SABER-seq in zebrafish. Also contains heat-shock activated mCherry reporter and CreERT2.
    Depositor
    Inserts
    BFP-SABER
    mCherry-t2A-CreERT2
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    Olactb and hsp70
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pAAV-Ef1a-fDIO-GCaMP6s

    Plasmid
    #105714
    Purpose
    Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.
    Depositor
    Has Service
    AAV8
    Insert
    GCaMP6s
    Use
    AAV; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a, Human elongation factor-1 alpha
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pTJV1Sc3-rpoB

    Plasmid
    #166983
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Insert
    RecT (recT E. coli)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pVvanCC
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pTOPO-col10a1-KI-donor

    Plasmid
    #184874
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Inserts
    Collagen10a1 5' homology arm
    Collagen10a1 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    eGFP
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pAAV-TRE-DIO-hM4Di-mCherry

    Plasmid
    #182532
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The hM4Di receptor expression requires both neural activity and Cre recombinase.
    Depositor
    Insert
    AAV transgene - hSyn promoter-tet-flex-hM4Di-cherry
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    hM4Di-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    tetO promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pAAV-TRE-DIO-Casp3-TEV

    Plasmid
    #183766
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.
    Depositor
    Insert
    AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pAAV-TRE-DIO-GCaMP6s

    Plasmid
    #183809
    Purpose
    his plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.
    Depositor
    Insert
    AAV transgene TetO promoter-DIO/flex-GCaMP6
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pAAV-TRE-DIO-ChR2-EYFP

    Plasmid
    #183765
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The ChR2-EYFP expression requires both neural activity and Cre recombinase.
    Depositor
    Insert
    AAV transgene -teto promoter-flex/dio-ChR2-EYFP
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    ChR2-EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    tetO promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40

    Plasmid
    #100833
    Purpose
    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G
    Promoter
    Synapsin
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
  10. TBG-Vector

    Plasmid
    #109396
    Purpose
    AAV-CRISPR knockout plasmid with Cre recombinase driven by TBG promoter for direct in vivo genome editing in mouse liver
    Depositor
    Type
    Empty backbone
    Use
    AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TBG
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pAAV.Syn.Flex.GCaMP6s.WPRE.SV40

    Plasmid
    #100845
    Purpose
    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G
    Promoter
    Synapsin
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
  12. pAAV.Syn.Flex.GCaMP6m.WPRE.SV40

    Plasmid
    #100838
    Purpose
    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter
    Has Service
    AAV1 and AAV9
    Insert
    GCaMP6m
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …
    Promoter
    Synapsin
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
  13. pAAV.CAG.Flex.GCaMP6m.WPRE.SV40

    Plasmid
    #100839
    Purpose
    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6m
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …
    Promoter
    CAG
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
  14. pJET-col2a1a-KI-donor

    Plasmid
    #184876
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka col2a1a locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Inserts
    Collagen2a1a 5' homology arm
    Collagen2a1a 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    EGFP
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. Lenti-LucAL

    Plasmid
    #174049
    Purpose
    Expresses mALG8 (ITYTWTRL) and mLAMA4 (VGFNFRTL) antigens as a fusion to luciferase and Cre recombinase
    Depositor
    Insert
    LucAL
    Use
    Cre/Lox, Lentiviral, and Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    ALG8 alanine 506 to threonine; LAMA4 glycine 1254…
    Promoter
    Human Ubiquitin C
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  16. Lenti-LucA

    Plasmid
    #174047
    Purpose
    Expresses mALG8 (ITYTWTRL) antigen as a fusion to luciferase and Cre recombinase
    Depositor
    Insert
    LucA
    Use
    Cre/Lox, Lentiviral, and Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    ALG8 alanine 506 to threonine
    Promoter
    Human Ubiquitin C
    Available since
    Availability
    Academic Institutions and Nonprofits only
  17. Lenti-LucL

    Plasmid
    #174048
    Purpose
    Expresses mLAMA4 (VGFNFRTL) antigen as a fusion to luciferase and Cre recombinase
    Depositor
    Insert
    LucL
    Use
    Cre/Lox, Lentiviral, and Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    LAMA4 glycine 1254 to valine
    Promoter
    Human Ubiquitin C
    Available since
    Availability
    Academic Institutions and Nonprofits only
  18. pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]

    Plasmid
    #190236
    Purpose
    AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase
    Depositor
    Insert
    EGFP
    Use
    AAV
    Tags
    F2A, LDLR C-terminal sequence, mRFP1, myristoylat…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 1351 - 1400 of 1454 results