We narrowed to 4,801 results for: PIR

1. pST39-pNL29

   Plasmid

   #91696

   Purpose

   Expresses Mega GVs in E.Coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Protoc. 2017 Oct;12(10):2050-2080. d

   Insert

   Mega GV gene cluster (from pNL29) in pST39 backbone
   

   Use

   Tags

   No tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. WT GBA pcDNA3.1

   Plasmid

   #188580

   Purpose

   Ectopic expression of wild-type GBA

   Depositor

   Anthony Schapira

   Article

   Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

   Insert

   GBA (GBA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. WT C-His Ana GvpC in pET28a

   Plasmid

   #85732

   Purpose

   Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

   Insert

   Gas vesicle protein C (his-tagged)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Added an extra glycine after the methionine at th…

   Promoter

   T7

   Available since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

   Plasmid

   #221075

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-SEMPER-mARG_ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry

   Plasmid

   #221080

   Purpose

   SEMPER plasmid for expression of gas vesicles using mammalian acoustic reporter genes (mARGs)

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK-caCas9-NatMX-Neut5L-Leu2 drive

   Plasmid

   #89578

   Purpose

   caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

   Depositor

   George Church

   Article

   Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

   Insert

   Leu2 gene drive
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

   Plasmid

   #221076

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

   Plasmid

   #221077

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

   Plasmid

   #221079

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2

    Plasmid

    #221082

    Purpose

    Plasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfGFP[r5M]_ACC-mEBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    CMV, T7

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

    Plasmid

    #104563

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1 (D832A)
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. araBAD-BCD20-ClpP-BCD17-ClpX

    Plasmid

    #153301

    Purpose

    Expresses clpX and clpP in E.Coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Inserts

    clpX (clpX Escherichia coli)
    clpP (clpP Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. N370S GBA pcDNA3.1

    Plasmid

    #188583

    Purpose

    Ectopic expression N370S GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N370S

    Promoter

    CMV

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. L444P GBA pcDNA3.1

    Plasmid

    #188582

    Purpose

    Ectopic expression L444P GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L444P

    Promoter

    CMV

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAP05

    Plasmid

    #186261

    Purpose

    sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

    Depositor

    Yongku Cho

    Article

    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

    Inserts

    sfGFP
    EL222 (ELI_04755 Erythrobacter litoralis)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. E326K GBA pcDNA3.1

    Plasmid

    #188581

    Purpose

    Ectopic expression E326K GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E326K

    Promoter

    CMV

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MBP-MEK1

    Plasmid

    #68300

    Purpose

    N-terminal MBP tagged human MEK1 gene for bacterial expression

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    MBP-MEK1 (MAP2K1 Synthetic, Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ana GvpC-SpyTag-His in pET28a

    Plasmid

    #85737

    Purpose

    Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (fused to SpyTag and His-tagged)
    

    Use

    Tags

    His-tag and SpyTag

    Expression

    Bacterial

    Mutation

    Added an extra glycine after the methionine at th…

    Promoter

    T7

    Available since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK-caCas9-NatMX-Neut5L-Ade2 drive

    Plasmid

    #89577

    Purpose

    caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Insert

    Ade2 gene drive
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. WT N-His Ana GvpC in pET28a

    Plasmid

    #153294

    Purpose

    Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C (N-terminal his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Added a hexahistidine tag after the methionine at…

    Promoter

    T7

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge