We narrowed to 18,853 results for: REV
-
Plasmid#61407PurposeExpresses human OTUD1 (OTU domain) in E. coli.DepositorInsertOTUD1 (OTUD1 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-286 and aa 438-481.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT9751-BEAR-mCherry-preedited
Plasmid#162993PurposeBEAR control plasmid with split mCherry and intact 5' splice siteDepositorInsertmCherry split with an intron between amino acids 119-120
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAVA-LGF2-Gal11p
Plasmid#127481PurposePositive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interactionDepositorInsertLGF2-Gal11p (GAL11 Budding Yeast)
ExpressionBacterialAvailable SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.1-AP-His
Plasmid#72042PurposeExpresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-MOB1A
Plasmid#172988Purposeconstitutive expression of FLAG-tagged MOB1ADepositorAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1045C
Plasmid#125005PurposeExpresses gRNAs targeting hid, eve, and winglessDepositorAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sema3f(L,+)-AP-His
Plasmid#72021PurposeExpresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains no deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax
Plasmid#173875PurposeMammalian expression of mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)DepositorInsertmVenus-NES-SspB-RGL2GEF-P2A-iLIDcaax
TagsC-terminal CAAX-box and mVenusExpressionMammalianPromoterCMVAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax
Plasmid#173876PurposeMammalian expression of mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)DepositorInsertmCherry-NES-SspB-RGL2GEF-P2A-iLIDcaax
TagsC-terminal CAAX-box and mCherryExpressionMammalianPromoterCMVAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-beta-actin
Plasmid#178076PurposeInsect cell expression of beta-actinDepositorInsertbeta-actin (ACTB Human)
ExpressionInsectAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV11K
Plasmid#63184PurposepRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUB2 (full-length, aa 1-234)
Plasmid#61421PurposeExpresses human OTUB2 (full-length) in E. coli.DepositorInsertOTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)
TagsHis6-GST-3CExpressionBacterialPromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCINeo-hFX-HA
Plasmid#14976DepositorAvailable SinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
ATAD2 D7.3 gRNA
Plasmid#90538Purpose3rd generation lentiviral gRNA plasmid targeting human ATAD2DepositorInsertATAD2 (Guide Designation D7.3)
UseCRISPR and LentiviralPromoterU6Available SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NOXO1-1/2
Plasmid#91499PurposeProtein expression and purification of human SH3 domain construct NOXO1-1/2DepositorInsertNOXO1-1/2 (NOXO1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB2903(XI-2 MarkerFree)
Plasmid#73275PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
KIF2A D7.2 gRNA
Plasmid#90720Purpose3rd generation lentiviral gRNA plasmid targeting human KIF2ADepositorInsertKIF2A (Guide Designation D7.2)
UseCRISPR and LentiviralPromoterU6Available SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
KIF2A D8.2 gRNA
Plasmid#90721Purpose3rd generation lentiviral gRNA plasmid targeting human KIF2ADepositorInsertKIF2A (Guide Designation D8.2)
UseCRISPR and LentiviralPromoterU6Available SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAP G116A
Plasmid#123081PurposeGateway entry clone encoding human GABARAP G116ADepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseGateway entry vector / entry cloneMutationGlycine 116 to AlanineAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i1 sgRNA / hSpCas9
Plasmid#172825PurposeMammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SHANK3-1/1
Plasmid#91421PurposeProtein expression and purification of human SH3 domain construct SHANK3-1/1DepositorInsertSHANK3-1/1 (SHANK3 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-TUBA1B_sgRNA
Plasmid#183889PurposepX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.DepositorAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Tmem214
Plasmid#175153PurposeLentiviral expression of V5-tagged mouse Tmem214DepositorAvailable SinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B G120A
Plasmid#123073PurposeGateway entry clone encoding human MAP1LC3B G120ADepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseGateway entry vector / entry cloneMutationGlycine 120 to AlanineAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
PLK1 G6.1 gRNA
Plasmid#90835Purpose3rd generation lentiviral gRNA plasmid targeting human PLK1DepositorInsertPLK1 (Guide Designation G6.1)
UseCRISPR and LentiviralPromoterU6Available SinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-POLArISact
Plasmid#164970PurposeT7 promotor drives in vitro transcription of POLArISact with a human kozak sequenceDepositorInsertPOLArISact
UseIn vitro transcriptionPromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
782 pMEActMEK
Plasmid#31166DepositorAvailable SinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-plk4
Plasmid#136788PurposeMammalian expression of the centrosomal protein plk4 N-terminally fused to SNAP-tagDepositorInsertSNAP-plk4 (PLK4 Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro
Plasmid#61441PurposeDox-inducible MyoD with mRNA tag for smFISHDepositorInsertMyoD (Myod1 Mouse)
UseLentiviralAvailable SinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAcrF2
Plasmid#89234PurposePlasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage siteDepositorInsertAcrF2
Tags6xHis-TEVAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAVA-Gal11p-LGF2
Plasmid#127480PurposePositive control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (RNAp associated) interactionDepositorInsertGal11p-LGF2 (GAL11 Budding Yeast)
ExpressionBacterialAvailable SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5
Plasmid#63210PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K4
Plasmid#63212PurposepCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHDR-eGFP (pXK-1647)
Plasmid#208824PurposeThe HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDRDepositorTypeEmpty backboneExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330 _HITI donor B6
Plasmid#172847PurposeDRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBK86 pHomL-RPL18Bp-preCOX4-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179070PurposeVector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Scarlet(rfp), a marker for the mitochondriaDepositorInsertRPL18Bp-preCOX4-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK68 pHomL-RPL18Bp-SRM1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179052PurposeVector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Scarlet(rfp), a marker for the nucleusDepositorInsertRPL18Bp-SRM1-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB3039(XII-2 MarkerFree)
Plasmid#73279PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
CEP55 B12.2 gRNA
Plasmid#90623Purpose3rd generation lentiviral gRNA plasmid targeting human CEP55DepositorInsertCEP55 (Guide Designation B12.2)
UseCRISPR and LentiviralPromoterU6Available SinceNov. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
CEP55 B11.2 gRNA
Plasmid#90622Purpose3rd generation lentiviral gRNA plasmid targeting human CEP55DepositorInsertCEP55 (Guide Designation B11.2)
UseCRISPR and LentiviralPromoterU6Available SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only