We narrowed to 18,395 results for: Tec

1. pOCC65

   Plasmid

   #118865

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease, mGFP

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV5 alpha 1A AR

   Plasmid

   #45760

   Depositor

   Robert Lefkowitz

   Article

   Schwinn et al J Biol Chem. 1990 May 15;265(14):8183-9.

   Insert

   alpha 1A AR (Adra1a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pRDB_156

   Plasmid

   #216090

   Purpose

   Cas9 [Sp] CRISPRa targeting CD274, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD274 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXNgate22-1HA

   Plasmid

   #105077

   Purpose

   Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

   Depositor

   Wolf Frommer

   Article

   Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

   Type

   Empty backbone

   Use

   Tags

   NubG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. MMLV-RT for Split-PE (pEH45)

   Plasmid

   #190105

   Purpose

   Expresses MMLV-RT (full-length, from PE2) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   MMLV-RT
   

   Use

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   RT pentamutant from PE2 (D200N, T306K, W313F, T33…

   Promoter

   CMV

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-CreiLOV-P2A-FusionRed

   Plasmid

   #192818

   Purpose

   Expresses CreiLOV with FusionRed tag in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   CreiLOV
   

   Use

   AAV

   Tags

   FusionRed

   Expression

   Mammalian

   Mutation

   Promoter

   chicken β-actin promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TALEN1257

   Plasmid

   #32280

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   TAL array targeting hey2 (hey2 Zebrafish)
   

   Use

   T7

   Tags

   3X FLAG and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMU-4

   Plasmid

   #172353

   Purpose

   CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA

   Depositor

   Eriko Takano

   Article

   Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

   Insert

   D4-TheoR-cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   D4

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCellFree_G03 CALR

   Plasmid

   #67086

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CALR (CALR Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pTn7·X-nia

    Plasmid

    #122592

    Purpose

    Plasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion site

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    xylS/Pm->Nia
    

    Use

    Synthetic Biology; Tn7 genomic integration

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21b-rrt-His8

    Plasmid

    #194088

    Purpose

    Expresses Ec86 reverse transcriptase in Escherichia coli

    Depositor

    Tingting Zou

    Article

    Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

    Insert

    Ec86 rrt
    

    Use

    Tags

    His8

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMV_AA090

    Plasmid

    #216099

    Purpose

    Knockout, EF1a-driven Cas12a (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas12a [EnAs]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSH1/Mf-del-Akt-E

    Plasmid

    #15288

    Depositor

    David Spencer

    Article

    Park et al Nat Biotechnol. 2006 Dec . 24(12):1581-9

    Insert

    delPH-Akt (Akt1 Mouse)
    

    Use

    Tags

    HA epitope and Myristoylation-targeting domain Fy…

    Expression

    Mammalian

    Mutation

    Truncated PH (residues 1-99)

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOCC292

    Plasmid

    #118881

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    mRuby3, HIS6, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL2622 (pCas7_Vch)

    Plasmid

    #200894

    Purpose

    Expresses human codon-optimized V. cholerae Cas7 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas7
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMM0638

    Plasmid

    #129005

    Purpose

    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOCC291

    Plasmid

    #118867

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease, mRuby3

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGWB508

    Plasmid

    #74850

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGS1222

    Plasmid

    #63791

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    eGFP-Twin-Strep tag followed by URA3
    

    Use

    E. coli cloning vector

    Tags

    eGFP-Twin-Strep tag

    Expression

    Mutation

    Promoter

    none

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB1H1-Zif268

    Plasmid

    #12612

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    Zif268 in pB1H1 (Egr1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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