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pEGFP-RhoA Biosensor

Plasmid

#68026

Purpose

C-terminus of Anillin in mammalian expression vector pEGFP-C1

Depositor

Michael Glotzer

Article

Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

Insert

Anillin (AHD+PH) (ANLN Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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TFORF2629

Plasmid

#142966

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

INSM2 (INSM2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-IRF2

Plasmid

#232642

Purpose

transient expresses IRF2 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

Insert

IRF2 (IRF2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

Plasmid

#169333

Purpose

Expression of HaloTag9 at the mitochondrial surface of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

TOMM20-HaloTag9 (TOMM20 Human)

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-SOX9

Plasmid

#141404

Purpose

Inducible Expression of SOX9

Depositor

Lorenz Studer

Article

Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

Insert

SOX9 (SOX9 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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FUGW-APOE-E4-T2A-mCherry

Plasmid

#190809

Purpose

Express APOE E4 allele from Ubiquitin C promoter, co-expressing mCherry

Depositor

Ronald Hart

Insert

APOE (APOE Human)

Use

Lentiviral

Mutation

E4

Promoter

Ubi C

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FIP200 (1-640)-msfGFP

Plasmid

#203546

Purpose

FIP200 NTD-GFP-10His mammalian expression

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Tags

msfGFP-10His

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-Calr-KDEL-IRES-Puromycin

Plasmid

#134664

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

calreticulin (CALR Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 18-413

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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TFORF2785

Plasmid

#143618

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GLIS1 (GLIS1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Cdc42-Q61L

Plasmid

#12974

Depositor

Gary Bokoch

Insert

Cdc42 constitutively active (CDC42 Human)

Tags

Myc

Expression

Mammalian

Mutation

Q61L Constitutively active

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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BRAF_pLX307

Plasmid

#98322

Purpose

Lentiviral expression of BRAF

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pTOM20-ddGFP-B

Plasmid

#40291

Depositor

Robert Campbell

Article

Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

Insert

TOMM20 (TOMM20 Human)

Tags

ddGFP-B

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-Anc689-UdgX-nCas9-RBMX

Plasmid

#163552

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-Anc689-UdgX-nCas9-RBMX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-YFP

Plasmid

#67146

Purpose

Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-YFP (ADRB2 Aequorea victoria, Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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EB3N-VVDfast-Halo-iLID

Plasmid

#190166

Purpose

Expresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

EB3N-VVDfast-Halo-iLID

Tags

-VVDfast-mClover3-iLID

Expression

Mammalian

Mutation

1-189 aa from EB3

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_TMPRSS2_ERG

Plasmid

#205943

Purpose

Express mEGFP-tagged fusion protein, TMPRSS2_ERG from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TMPRSS2_ERG (TMPRSS2, ERG Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_MYC_WT

Plasmid

#82927

Purpose

Gateway Donor vector containing MYC, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

Myc (MYC Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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mCer-TG2-YFP

Plasmid

#85471

Purpose

TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET

Depositor

Jeffrey Keillor, Ray Truant

Article

Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371

Insert

hTG2 (TGM2 Human)

Tags

EYFP and mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-PAK3

Plasmid

#23439

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK3 (PAK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO/GFP-TTP

Plasmid

#141190

Purpose

Expresses GFP-TTP in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

TTP (ZFP36 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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