164,629 results

1. AICSDP-7:SEC61B-mEGFP

   Plasmid

   #87426

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   SEC61B Homology Arms with mEGFP-linker (SEC61B Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pAAV FLEX Synaptophysin GFP

   Plasmid

   #137188

   Purpose

   Cre-dependent expression of synaptophysin-GFP

   Depositor

   Matthew Nolan

   Article

   Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

   Insert

   Synaptophysin GFP (Syp Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmScarlet_peroxisome_C1

   Plasmid

   #85063

   Purpose

   In vivo visualization of the peroxisome (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   Peroxisomal targeting sequence
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE-ESR1-Y537N

   Plasmid

   #116373

   Purpose

   Lentiviral expression of ESR1 Y537N

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ESR1 (ESR1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Y537N

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pQLL-MAG[EF1α-copGFP]

   Plasmid

   #218324

   Purpose

   Donor plasmid of MAG Transposon with copGFP mammalian expression cassette

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   copGFP mammalian expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. negMaLionB

   Plasmid

   #113911

   Purpose

   negative control for Blue ATP sensor

   Depositor

   Tetsuya Kitaguchi

   Article

   Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

   Insert

   negMaLionB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMRX-IB-TMEM192-mGFP

   Plasmid

   #184897

   Purpose

   Stable expression of TMEM192-mGFP in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Tags

   monomeric EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTK296

   Plasmid

   #59565

   Purpose

   SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP-PEST

   Depositor

   Ron Weiss

   Article

   Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

   Insert

   SGP(14) 2xK-turn Kozak EGFP-PEST
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. WT TXNIP

   Plasmid

   #200088

   Purpose

   Dox-inducible WT human TXNIP in pInducer20 vector

   Depositor

   Jason MacGurn

   Article

   Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

   Insert

   TXNIP (TXNIP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PD22

    Plasmid

    #164531

    Purpose

    Expresses Smo-FLAG

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HA-HIF2alpha-P405A/P531A-pcDNA3

    Plasmid

    #18956

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-ER-EBFP2

    Plasmid

    #231183

    Purpose

    Expresses control plasmid for ER-mitochondrial linkers, tagged with EBFP2, in mammalian cells

    Depositor

    Dmitry Lim

    Article

    Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

    Insert

    ER-EBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RF-88-Ate1

    Plasmid

    #79187

    Purpose

    Expression of C-terminally Flag tagged ATE1 in mammalian cells

    Depositor

    Richard Fahlman

    Insert

    ATE1 (ATE1 Human)
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby

    Plasmid

    #71760

    Purpose

    mGFP and Synaptophysin-mRuby expression from Cre-expressing neurons

    Depositor

    Liqun Luo

    Article

    Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

    Has Service

    AAV1

    Inserts

    mGFP
    Synaptophysin
    

    Use

    AAV and Cre/Lox

    Tags

    Palmitoylation signal and mRuby

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-Flag-NPM1c

    Plasmid

    #131813

    Purpose

    Expresses Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    This is the cytoplasmic mutant causing AML, where…

    Promoter

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

    Plasmid

    #118593

    Purpose

    Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha/TRE3G

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-mCardinal

    Plasmid

    #51311

    Purpose

    Expresses far-red fluorescent protein mCardinal in mammalian cells

    Depositor

    Michael Lin

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    mCardinal
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNAintron-LASV-GPC

    Plasmid

    #215690

    Purpose

    Produces the Lassa virus glycoprotein

    Depositor

    Melinda Brindley

    Article

    Acciani et al J Virol. 2017 Aug 24;91(18):e00574-17. d

    Insert

    LASV GP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pHR'CMVGFP_NLS_hRIF1(1-1308)

    Plasmid

    #23134

    Depositor

    Lifeng Xu

    Article

    Xu et al J Cell Biol. 2004 Dec 6. 167(5):819-30.

    Insert

    RIF1 deletion construct (RIF1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. V405 4HA-CKIe

    Plasmid

    #13724

    Depositor

    David Virshup

    Article

    Rivers et al J Biol Chem. 1998 Jun 26. 273(26):15980-

    Insert

    casein kinase 1 epsilon (CSNK1E Human)
    

    Use

    Tags

    4 HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge