We narrowed to 5,834 results for: pcas

1. pX458_sgAgo1_Nterm

   Plasmid

   #170921

   Purpose

   Expresses spCas9 and sgRNA targeting the N-terminus of Ago1 for N-terminal tagging

   Depositor

   Constance Ciaudo

   Article

   Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

   Insert

   sgAgo1 (Ago1 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PEA1-Nuclease

   Plasmid

   #176528

   Purpose

   Delivers all prime editing nuclease components in a single plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   GC to CA point mutation in SpCas9 to restore nucl…

   Promoter

   CbH for Cas9, hU6 for gRNAs

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR207-MUM2sp-Citrine-ADPG2

   Plasmid

   #170725

   Purpose

   Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

   Depositor

   George Haughn

   Article

   McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

   Insert

   ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
   

   Use

   Gateway donor vector / entry clone

   Tags

   Citrine tag (714bp)

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR207-MUM2sp-ADPG2

   Plasmid

   #170723

   Purpose

   Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

   Depositor

   George Haughn

   Article

   McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

   Insert

   ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
   

   Use

   Gateway donor vector / entry clone

   Available Since

   Nov. 11, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pEGB_3alpha2 OplacI:mini35S:RDF:Tnos (GB1534)

   Plasmid

   #160617

   Purpose

   TU for the regulated expression of the PhiC31 phage recombination directionality factor (RDF) gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   RDF
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   mini35s

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGB3 alpha2 P35S:MS2-VP64:Tnos (GB1395)

   Plasmid

   #160609

   Purpose

   TU for the constitutive expresion of the viral coat protein MS2 fused on C-terminal with the VP64

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35S:MS2-VP64:Tnos
   

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB1529)

   Plasmid

   #160614

   Purpose

   TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LexA operator fused to the minimal 35S promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   PhiC31
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   mini35s

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGB_3alpha1 OplacI:mini35S:phiC31:Tnos (GB1530)

   Plasmid

   #160615

   Purpose

   TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   PhiC31
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   mini35s

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. BE3-YE1

   Plasmid

   #132943

   Purpose

   Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

   Depositor

   Hui Yang

   Article

   Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

   Insert

   BE3-(W90Y-R126E)
   

   Use

   CRISPR; Base editor

   Expression

   Mammalian

   Mutation

   BE3-(W90Y-R126E)

   Available Since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330_gRNA_H19-DMR-2

    Plasmid

    #159930

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, CBh

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330_gRNA_H19-DMR-1

    Plasmid

    #159929

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, CBh

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJT175_GalL_A3A(Y130F)Δ186-BE3

    Plasmid

    #145124

    Purpose

    Expressing base editor A3A(Y130F)Δ186-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A(Y130F)Δ186-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    A3A(Y130F; 1-186AA); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTBL405 CHYRON1 integration construct

    Plasmid

    #126442

    Purpose

    To integrate the CHYRON1 locus at HEK293site3.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON1 hgRNA
    pCMV-puro
    

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. mei-S332-GFP 5.6kb genomic DNA in Casper4 T331D

    Plasmid

    #112986

    Purpose

    P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331D) into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

    Insert

    mei-S332 (mei-S332 Fly)
    

    Use

    P insertion vector

    Tags

    GFP

    Mutation

    T331D

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. mei-S332-GFP 5.6kb genomic DNA in Casper4 T331A

    Plasmid

    #112988

    Purpose

    P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331A) into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

    Insert

    mei-S332 (mei-S332 Fly)
    

    Use

    P insertion vector

    Tags

    GFP

    Mutation

    T331A

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. JG693: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X3)

    Plasmid

    #104570

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to three DmrA domains

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1(D832A)-DmrA(x3)
    

    Tags

    3x FLAG, 3x HA, and NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MST1R gRNA (BRDN0001487060)

    Plasmid

    #78050

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MST1R

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MST1R (MST1R Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. MST1R gRNA (BRDN0001145791)

    Plasmid

    #78051

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MST1R

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MST1R (MST1R Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. MST1R gRNA (BRDN0001145169)

    Plasmid

    #78052

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MST1R

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MST1R (MST1R Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. W45A mutant of H3K9me3 biosensor

    Plasmid

    #120808

    Purpose

    FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
    

    Expression

    Mammalian

    Mutation

    Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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