We narrowed to 10,337 results for: plasmids 101

1. Bxb1-GA_CAG-rtTA-T2A-mTagBFP2

   Plasmid

   #229797

   Purpose

   Bxb1-GA donor plasmid for constitutive expression of reverse tetracycline transactivator (rtTA) and nuclear mTagBFP2

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.3-SARS2-B.1.617.1

   Plasmid

   #172319

   Purpose

   expressing SARS-CoV-2 B.1.617.1 (kappa strain) spike protein for pseudovirus production

   Depositor

   David Nemazee

   Article

   Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

   Insert

   Spike of B.1.617.1 strain (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…

   Promoter

   CMV

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.3_SARS2_omicron_BA.2.75

   Plasmid

   #190674

   Purpose

   expressing SARS-CoV-2 BA.2.75 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Article

   Dacon et al Cell Host Microbe. 2022 Nov 4. pii: S193

   Insert

   Spike (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K147E, W152R, F157L, I210V, G257S, D339H, G446S, …

   Promoter

   CMV

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVRC8400-SARS-CoV-2-RBD-L455RA475RG502R-AVI

   Plasmid

   #160480

   Purpose

   Expresses SARS-CoV-2 RBD-L455RA475RG502R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

   Insert

   SARS-CoV-2-RBD-L455RA475RG502R
   

   Use

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   changed Leucine 455 to Arginine, Alanine 475 to A…

   Promoter

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVRC8400-SARS-CoV-2-RBD-L455RG496R-AVI

   Plasmid

   #160479

   Purpose

   Expresses SARS-CoV-2 RBD-L455RG496R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

   Insert

   SARS-CoV-2-RBD-L455RG496R
   

   Use

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   changed Leucine 455 to Arginine and changed Glyci…

   Promoter

   Available Since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVRC8400-SARS-CoV-2-RBD-L455RA475R-AVI

   Plasmid

   #160478

   Purpose

   Expresses SARS-CoV-2 RBD-L455RA475R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

   Insert

   SARS-CoV-2-RBD-L455RA475R
   

   Use

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   changed Leucine 455 to Arginine and changed Alani…

   Promoter

   Available Since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_AKT1_WT

   Plasmid

   #81764

   Purpose

   Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_AKT1_WT

   Plasmid

   #82294

   Purpose

   Gateway Donor vector containing AKT1, part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_AKT1_p.L52R

   Plasmid

   #81604

   Purpose

   Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   L52R

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_AKT1_p.E267G

    Plasmid

    #81561

    Purpose

    Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    E267G

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE-hLin28B

    Plasmid

    #26358

    Depositor

    George Daley

    Article

    Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

    Insert

    lin-28 homolog B (LIN28B Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Nov. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_AKT1_p.E17K

    Plasmid

    #82089

    Purpose

    Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    E17K

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_AKT1_p.E17K

    Plasmid

    #81459

    Purpose

    Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    E17K

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_AKT1_p.Q79K

    Plasmid

    #81479

    Purpose

    Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Q79K

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_AKT1_p.D44N

    Plasmid

    #81456

    Purpose

    Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    NM_005163.2 (AKT1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    D44N

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_AKT1_p.V201I

    Plasmid

    #81453

    Purpose

    Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    V201I; p.117delE

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223_AKT1_p.R370C

    Plasmid

    #81416

    Purpose

    Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    R370C; p.114delE

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-DIO-BACE-HA

    Plasmid

    #232353

    Purpose

    Beta-Secretase to be expressed (1:1) with either ATLAS protein.

    Depositor

    Don Arnold

    Article

    Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

    Insert

    BACE (BACE1 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pαH-S-GSAS-2P-OMICRON

    Plasmid

    #180593

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS SARS-CoV-2 BA.2 Spike

    Plasmid

    #185604

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.2 Spike

    Depositor

    Marceline Côté

    Article

    Tauzin et al Cell Rep. 2022 Oct 25;41(4):111554. doi:

    Insert

    SARS-CoV-2 BA.2 Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19I, L24S, Δ25-27, G142D, V213G, G339D, S371F, S…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available Since

    July 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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