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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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20,128 results

Showing: 8461 - 8490 of 20128 results

  1. pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)

    Plasmid
    #103849
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    CMV
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103849-plasmid-map-sequence-id-199152

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  14. pTriEx-HTNC

    Plasmid
    #13763
    Purpose
    For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.
    Depositor
    Klaus Rajewsky
    Article
    Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99
    Insert
    His-TAT-NLS-Cre
    Use
    Baculovirus
    Tags
    His Tag, NLS, and TAT
    Expression
    Bacterial
    Promoter
    p10 promoter
    Available Since
    April 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13763-plasmid-map-sequence-id-364424

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  23. Lamp1-YFP

    Plasmid
    #1816
    Purpose
    Mammalian expression of Lamp1 fused to YFP. Used for localization to the lysosome.
    Depositor
    Walther Mothes
    Article
    Sherer et al Traffic 2003 Nov;4(11):785-801.
    Insert
    lysosome associated membrane protein 1 (Lamp1 Rat)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    D50E (not important for function of plasmid)
    Available Since
    Feb. 16, 2006
    Availability
    Academic Institutions and Nonprofits only
    1816-plasmid-map-sequence-id-364671

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  25. GCEPIA1-SNAPER

    Plasmid
    #172659
    Purpose
    Detect the real-time ER calcium store and local calcium microdomains in live cells.
    Depositor
    Pingyong Xu
    Article
    Luo et al Biophys Rep (2019) 5, 31–42
    Insert
    calmodulin
    Expression
    Mammalian
    Mutation
    Please see depositor comments
    Available Since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    172659-plasmid-map-sequence-id-339177

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  27. pET28-MHL

    Plasmid
    #26096
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26096-plasmid-map-sequence-id-326568

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  29. pX330-BsaIx2-DD-Cas9

    Plasmid
    #75380
    Purpose
    Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1
    Depositor
    Michele Calos
    Article
    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv
    Insert
    DD-SpCas9
    Use
    CRISPR
    Tags
    FKBP12 L106P DD
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    75380-plasmid-map-sequence-id-192102

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Showing: 8461 - 8490 of 20128 results