We narrowed to 24,502 results for: crispr

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pN-PITCh-HF

Plasmid

#127882

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3F tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

Plasmid

#223175

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

hU6_Rfxcas13d-DR1_BsmBI

Use

Lentiviral

Mutation

DR1 A>T

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAID6

Plasmid

#136989

Purpose

Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4

Depositor

Huimin Zhao

Article

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Inserts

dLbCpf1-VP
dSpCas9-RD1152
SaCas9
Csy4

Expression

Yeast

Promoter

ENO2, TDH3, TEF1, and TPI1

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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3 gRNA concatemer

Plasmid

#84880

Purpose

Empty concatmer vector in which 3 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only
pYPQ131D

Plasmid

#69287

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

Plasmid

#225755

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Blasto

Plasmid

#167904

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pRec ΔLacI

Plasmid

#104575

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCRA03

Plasmid

#103141

Purpose

Triple gRNA expression separated with 28nt Csy4 motif

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

gRNAs

Expression

Yeast

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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U6-(BbsI)sgRNA_CAG-Venus-bpA

Plasmid

#86985

Purpose

For cloning and expression of sgRNA together with Venus expression

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Venus

Expression

Mammalian

Mutation

Venus GFP variant

Promoter

CAG

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-BE3∆UGI

Plasmid

#96943

Purpose

The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).

Depositor

Daesik Kim

Article

Kim et al Nat Biotechnol. 2017 May;35(5):475-480.

Insert

BE3∆UGI

Tags

His6 tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pJG80

Plasmid

#89282

Purpose

Entry vector containing TaCas9-nos terminator between attL1 and attR5 sites

Depositor

Daniel Voytas

Article

Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

Insert

TaCas9

Use

CRISPR

Expression

Plant

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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Reckleen_2_SpGCas9

Plasmid

#233457

Purpose

Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 2 RECKLEEN plasmid

Use

CRISPR

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mStayGold-Puro

Plasmid

#227253

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

Plasmid

#222994

Purpose

Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Promoter

pCAG

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAC-U63-gRNA2.1

Plasmid

#170512

Purpose

gRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-MCS-P2A-GFP-PuroR

Plasmid

#107186

Purpose

Base plasmid for cloning gene duplication alleles with eGFP reporter.

Depositor

Richard Sherwood

Article

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Type

Empty backbone

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRB1081

Plasmid

#71479

Purpose

Expresses gRNA for dpy-10 conversion using NGAG PAM and VQR Cas9 variant

Depositor

Andrew Fire

Article

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

dpy-10 gRNA

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_RV6_empty

Plasmid

#203811

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)

Plasmid

#114081

Purpose

CAG promoter expression plasmid for human codon optimized enAsBE1.1

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)

Tags

SV40 NLS

Expression

Mammalian

Mutation

E174R, S542R, K548R and D908A, human codon optimi…

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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