We narrowed to 19,084 results for: iNOS

1. UBQLN2_450-624W

   Plasmid

   #176852

   Purpose

   Expresses human UBQLN2 construct 450-624 with added C-terminal W in bacterial cells

   Depositor

   Carlos Castañeda

   Article

   Dao et al Mol Cell. 2018 Mar 15;69(6):965-978.e6.

   Insert

   UBQLN2_450C_W (UBQLN2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deletion of residues 1-449 and addition of C-term…

   Promoter

   T7

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

   Plasmid

   #194684

   Purpose

   CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_06-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCALPS-Flag-Mili-EGFP

   Plasmid

   #212576

   Purpose

   Lentiviral expression of Flag-tagged mouse Mili in mammalian cells

   Depositor

   Phillip Zamore

   Article

   Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

   Insert

   Mili (Piwil2 Mouse)
   

   Use

   Lentiviral

   Tags

   5x Flag and SNAP tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYES2-gRNA-hyg-MCS

   Plasmid

   #107734

   Purpose

   E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.

   Depositor

   Sheng Yang

   Article

   Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

   Insert

   X-3 site gRNA expression cassettes
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52p

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-SLC35B2-sgRNA

   Plasmid

   #154860

   Purpose

   Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   SLC35B2 sgRNA (SLC35B2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-Dest47- WTN23C11orf83-GFP

   Plasmid

   #65845

   Purpose

   Mammalian expression of the wild type N terminal part (N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

   Depositor

   Amos Bairoch

   Article

   Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

   Insert

   Transmembrane (23 AA) of UQCC3 (UQCC3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   WT Transmembrane

   Promoter

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. sg1+sg2+sg3

   Plasmid

   #113969

   Purpose

   Triple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   sg1+sg2+sg3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. FN 108

   Plasmid

   #50495

   Depositor

   Kenneth Yamada

   Article

   Aota et al J Biol Chem. 1994 Oct 7;269(40):24756-61

   Insert

   fibronectin (FN1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   repeats 9 and 10; EcoR1 site on III-9 is mutated,…

   Promoter

   T7

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-mNRDc-E>A-V5

   Plasmid

   #51298

   Purpose

   expression of the inactive mutant of mouse NRDc in mammalian cells

   Depositor

   Eiichiro Nishi

   Article

   Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

   Insert

   NRDc E247A (Nrd1 Mouse)
   

   Use

   Tags

   HIS and V5

   Expression

   Mammalian

   Mutation

   E247A, enzymatic inactive mutant

   Promoter

   CMV

   Available Since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

10. FLAG-β-arrestin2 RRK/Q

    Plasmid

    #38261

    Depositor

    Robert Lefkowitz

    Article

    Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

    Insert

    β-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Arg233, Arg237, and Lys251 converted to Glutamines

    Promoter

    CMV

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

11. GFP-RNF110

    Plasmid

    #126547

    Purpose

    Expresses GFP-tagged RNF110 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RNF110 (PCGF2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. L-YARS2-9

    Plasmid

    #166533

    Purpose

    scFv of a human scaffold targeting Tyrosyl-tRNA synthetase 2, mitochondrial. Antigen coverage aa 31-477 of 477

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. -962 human cyclin D1 promoter TCF (0)-(4) sites mutant pGL3Basic

    Plasmid

    #32737

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -539 TCF(0) ATGAAAG deletion; -75 TCF(1) and -68 …

    Promoter

    -962 CCND1

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pDONR223 GABARAP G116A

    Plasmid

    #123081

    Purpose

    Gateway entry clone encoding human GABARAP G116A

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Glycine 116 to Alanine

    Promoter

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEJS1026-pMCSG7-HpaCas9

    Plasmid

    #121540

    Purpose

    Expresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    HpaCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRX-IPU-FLAG-NEK9T210A

    Plasmid

    #168270

    Purpose

    Expresses kinase-dead NEK9 mutant in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

    Insert

    NEK9 (NEK9 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed threonine 210 to alanine

    Promoter

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEJS1027-pMCSG7-SmuCas9

    Plasmid

    #121541

    Purpose

    Expresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    SmuCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBEST-p70a-UTR3-deGFP-6xHis-T500

    Plasmid

    #92224

    Purpose

    Expression of deGFP using the p70a promoter and UTR3

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    deGFP
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSG5-Myr-FLAG-p110α-DM

    Plasmid

    #55713

    Purpose

    eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    p110 alpha (Pik3ca Mouse)
    

    Use

    Tags

    myristoylation tag (Myr-FLAG)

    Expression

    Mammalian

    Mutation

    T207D, K227A

    Promoter

    T7

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

20. O-MARS-11

    Plasmid

    #166541

    Purpose

    scFv of a human scaffold targeting Methionyl-tRNA synthetase. Antigen coverage aa 1-225 of 900

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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