Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 26,659 results for: STI

Showing: 11361 - 11400 of 26659 results

  2. pGEM-2t

    Plasmid
    #159752
    Purpose
    CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    sgRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159752-plasmid-map-sequence-id-313824

    Enlarge

  3. pYEE

    Plasmid
    #159750
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159750-plasmid-map-sequence-id-313819

    Enlarge

  6. pYUU

    Plasmid
    #159751
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159751-plasmid-map-sequence-id-313821

    Enlarge

  12. pUt-mPF-GFP

    Plasmid
    #199222
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    rtTA-Advanced::P2A::EGFP
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    tight TRE promoter
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199222-plasmid-map-sequence-id-396540

    Enlarge

  13. pAAV-hSyn-GRAB_VIP1.0

    Plasmid
    #208681
    Purpose
    Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in neurons
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available Since
    Oct. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    208681-plasmid-map-sequence-id-410348

    Enlarge

  14. pBZ202-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Sa163RT-NLS-T2A-mCherry

    Plasmid
    #170184
    Purpose
    Expression vector for a sgRNA against BFP and spCas9-Sa163 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Sa163 msr-msd region by SpeI and AvrII.
    Depositor
    Hunter Fraser
    Article
    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris
    Inserts
    Sa163 RT
    Retron Sa163 msr-msd
    BFP-to-GFP conversion donor template An
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and hU6
    Available Since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    170184-plasmid-map-sequence-id-342530

    Enlarge

  29. pCW57.1_SPTLC1_C133W

    Plasmid
    #181921
    Purpose
    3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133W
    Depositor
    Christian Metallo
    Article
    Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10
    Insert
    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)
    Use
    Lentiviral; Mammalian expression, , doxycycline i…
    Tags
    Expression
    Mammalian
    Mutation
    cysteine at position 133 of the coding region of …
    Promoter
    tight TRE promoter
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    181921-plasmid-map-sequence-id-361675

    Enlarge

  35. pLenti GW V5 Eco/Dam mse LaminA

    Plasmid
    #182674
    Purpose
    Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.
    Depositor
    Bas van Steensel
    Article
    Meuleman et al Genome Res. 2013 Feb;23(2):270-80. doi:
    Insert
    Lamin A (Lmna Mouse)
    Use
    Lentiviral
    Tags
    E. Coli Dam and V5
    Expression
    Mammalian
    Mutation
    Promoter
    HSP
    Available Since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182674-plasmid-map-sequence-id-358371

    Enlarge

  36. pCS2+-Y276H PHYB-CAAX

    Plasmid
    #154911
    Purpose
    1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.
    Depositor
    Clare Buckley, Jonathan Clarke
    Article
    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:
    Insert
    Phytochrome B (PHYB Mustard Weed)
    Use
    Xenopus/avian/zebrafish
    Tags
    CAAX membrane moiety and mCherry
    Expression
    Mammalian
    Mutation
    1-621 amino acids of Arabidopsis PHYB, Tyrosine r…
    Promoter
    CMV
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154911-plasmid-map-sequence-id-308717

    Enlarge

Showing: 11361 - 11400 of 26659 results