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pCas-pML-1B_6xHis-Cas12m

Plasmid

#192282

Purpose

MmCas12m fused to His-tag for protein purification

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmCas12m

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 ORF3B

Plasmid

#141272

Purpose

Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF3B

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-SIRPB1

Plasmid

#116790

Purpose

Lentiviral expression of SIRPB1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SIRPB1 (SIRPB1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX SHIP1-SH2

Plasmid

#46492

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHIP1 (INPP5D Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-118

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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TrpC5 scFv [N67/15]

Plasmid

#190564

Purpose

Mammalian Expression of TrpC5 scFV. Derived from hybridoma N67/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

TrpC5 (Homo sapiens) recombinant scFV (TRPC5 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-deltaNp53:WTp53

Plasmid

#49244

Purpose

expresses human p53 deltaN linked to full length p53 with C terminal His tag and also expresses EGFP in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 deltaN and p53 WT (TP53 Human)

Tags

His and IRES-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pInducer-TAZ-S89A

Plasmid

#213588

Purpose

Doxycycline inducible expression of TAZ-S89A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

2xHA

Expression

Mammalian

Mutation

S89A

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-OXA1L

Plasmid

#116768

Purpose

Lentiviral expression of OXA1L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

OXA1L (OXA1L Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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VGlut2 scFv [N29/29]

Plasmid

#182085

Purpose

Mammalian Expression of VGlut2 scFv. Derived from hybridoma N29/29.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting VGlut2 (Rattus norvegicus) (Slc17a6 Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd7

Plasmid

#102869

Purpose

Expresses mouse Fzd7 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 7 (Fzd7 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PPO-mScarlet

Plasmid

#139503

Purpose

Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Tags

mScarlet following Gly-Gly-Ser linker

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

Plasmid

#154196

Purpose

Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

sgRNA-BC1-A, sgRNA-BC1-C

Use

Lentiviral; Catch barcode activation

Expression

Mammalian

Promoter

hU6 and mU6

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLIB_GST-TEV-SRC(WT)

Plasmid

#223742

Purpose

Expression of recombinant protein for purification, codon optimized for Insect cells

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

Src Kinase (SRC Human)

Tags

GST-TEV

Expression

Insect

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-TRPS1-P2A-mCherry

Plasmid

#203851

Purpose

Tet-inducible lentiviral plasmid expressing TRPS1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

TRPS1 (TRPS1 Human)

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-GFP-EWSR1-myc-His

Plasmid

#46385

Purpose

expresses EGFP tagged EWSR1 in mammalian cells

Depositor

Heinz Gehring

Article

Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

Insert

EWSR1 (EWSR1 Human)

Tags

EGFP, His, and myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
pC1GFP-Kir7.1

Plasmid

#79118

Purpose

Express N-terminal fused Kir7.1 protein in mammalian cells

Depositor

Bikash Pattnaik

Article

Pattnaik et al PLoS One. 2013 Aug 19;8(8):e71744. doi:

Insert

Inwardly rectifying potassium channel KCNJ13 (KCNJ13 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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CIRTS-control

Plasmid

#213788

Purpose

CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.

Depositor

Timothy Bredy

Article

Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:

Insert

CIRTS-Calm3-U6-control gRNA

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

Syn1, U6

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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Parvalbumin scFv [L114/3]

Plasmid

#190504

Purpose

Mammalian Expression of Parvalbumin scFV. Derived from hybridoma L114/3.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Parvalbumin (Rattus norvegicus) recombinant scFV (Pvalb Mouse)

Tags

HA, 6xHis, Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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MYC-PGK-blast

Plasmid

#190618

Purpose

Lentiviral expression plasmid of human MYC, blast selection

Depositor

Junwei Shi

Article

Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-SaPE2-P2A-BSD

Plasmid

#174818

Purpose

Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

SaPE2-P2A-BSD

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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