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We narrowed to 6,235 results for: KIT

Showing: 5701 - 5720 of 6235 results
  1. CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA

    Plasmid
    #55202
    Purpose
    Plasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Insert
    dsRed2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. pJW2098

    Plasmid
    #163094
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Insert
    30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. p425_Cas9_gRNA_LEU_1014a

    Plasmid
    #87407
    Purpose
    p425_Cas9_gRNA-ARS1014a All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, LEU2 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. p426_Cas9_gRNA-YPRCd15c

    Plasmid
    #87404
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting YPRCd15c sequence AATCCGAACAACAGAGCATA in yeast chromosome 14.
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting YPRCd15c
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. GABA(A) receptor subunit g2SE

    Plasmid
    #49170
    Purpose
    pHluorin-tagged GABA A receptor subunit (gamma 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Insert
    GABA(A) receptor subunit gamma 2 (Gabrg2 Mouse)
    Use
    Tags
    myc, pHGFP (pHluorin), and thrombin cleavage site…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
  6. GABA(A) receptor subunit B3SE

    Plasmid
    #49171
    Purpose
    pHluorin-tagged GABA A receptor subunit (beta 3) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Insert
    GABA(A) receptor subunit beta 3 (Gabrb3 Mouse)
    Use
    Tags
    myc, pHGFP (pHluorin), and thrombin cleavage site…
    Expression
    Mammalian
    Mutation
    GTG (V) to TGC (C)
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
  7. pOD1988-epiDEG

    Plasmid
    #89357
    Purpose
    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pdpy-7 (epidermis specific).
    Depositor
    Insert
    vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    vhhGFP4 (GFP nanobody)
    Expression
    Mutation
    Promoter
    Pdpy-7
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pJW2171

    Plasmid
    #163095
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Insert
    30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pOD2044-intDEG

    Plasmid
    #89366
    Purpose
    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pelt-2 (intestine specific).
    Depositor
    Insert
    vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    vhhGFP4 (GFP nanobody)
    Expression
    Mutation
    Promoter
    Pelt-2
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
  10. p426_Cas9_gRNA-ARS208a

    Plasmid
    #87383
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS208a sequence GTCCGCTAAACAAAAGATCT in yeast chromosome 2.
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS208a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 5701 - 5720 of 6235 results