Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

50+ requests Clear filter

Applied Filters

50+ requests Clear filter

8,969 results

Showing: 8581 - 8610 of 8969 results

  1. pJFA344C7

    Plasmid
    #49236
    Purpose
    TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    TALE-TET1CD Backbone (TET1 Synthetic, Human)
    Use
    TALEN; Tale-tet1 expression vector
    Promoter
    EF1a
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    49236-plasmid-map-sequence-id-72802

    Enlarge

  4. MSP2440

    Plasmid
    #72250
    Purpose
    Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72250-plasmid-map-sequence-id-131871

    Enlarge

  6. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

    Plasmid
    #64217
    Purpose
    Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A
    Depositor
    Ralf Kuehn
    Article
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Inserts
    Cas9
    hU6 promoter; BbsI sites for sgRNA
    H1 promoter; BamHI site for shRNA
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Promoter
    CBh, H1, and U6
    Available Since
    Sept. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    64217-plasmid-map-sequence-id-123057

    Enlarge

  17. pWPT-nlsLacZ

    Plasmid
    #12261
    Depositor
    Didier Trono
    Insert
    LacZ
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12261-plasmid-map-sequence-id-7273

    Enlarge

  28. p079 pPNT6

    Plasmid
    #11072
    Depositor
    Ramesh Shivdasani
    Type
    Empty backbone
    Use
    Cre/Lox
    Expression
    Mammalian
    Available Since
    April 26, 2006
    Availability
    Academic Institutions and Nonprofits only
    11072-plasmid-map-sequence-id-6886

    Enlarge

Showing: 8581 - 8610 of 8969 results