19,654 results

1. FusionRed-Fascin-C-10

   Plasmid

   #56117

   Purpose

   Localization: Cytoskeleton, Excitation: 580, Emission: 608

   Depositor

   Michael Davidson

   Article

   Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

   Insert

   Fascin (FSCN1 Human)
   

   Tags

   FusionRed

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

   Plasmid

   #125256

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection

   Depositor

   Roger D. Kamm

   Article

   Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

   Insert

   hChR2(H134R)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   EF1a-forward

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOpen-DpnIR

   Plasmid

   #165529

   Purpose

   Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   DpnI
   

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. AAV-hSynI-rtTAV16

   Plasmid

   #102367

   Purpose

   AAV vector to drive TET-ON activator expression from human synapsin I promoter

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Front Neural Circuits. 2014 Sep 17;8:110

   Insert

   rtTAV16
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   rtTA variant reported in Gene Therapy (2006) 13, …

   Promoter

   human synapsin I promoter

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-Neo-M2rtTA

   Plasmid

   #60843

   Purpose

   AAVS1 donor vector for genomic targeting

   Depositor

   Rudolf Jaenisch

   Article

   DeKelver et al Genome Res. 2010 Aug;20(8):1133-42. doi:

   Inserts

   M2rtTA
   Neo
   

   Use

   Targeting donor

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Noggin

   Plasmid

   #163586

   Purpose

   Expresses Noggin in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Nog (Nog Mouse)
   

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-hCRM1

   Plasmid

   #17647

   Depositor

   Xin Wang

   Article

   Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

   Insert

   CRM1 (XPO1 Human)
   

   Tags

   3x Flag

   Expression

   Mammalian

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330A-nBRD4/PITCh

   Plasmid

   #91794

   Purpose

   PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   SpCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chicken β-actin promoter

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-DsRed_IRES_EGFP

   Plasmid

   #92194

   Purpose

   Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   DsRed-IRES-EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. HR_G3BP1-V5-APEX2-GFP

    Plasmid

    #105284

    Purpose

    homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

    Depositor

    Eugene Yeo

    Article

    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

    Insert

    G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 Human, soybean)
    

    Use

    Human homologous recombination donor

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

    Plasmid

    #132398

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3 2 kb prom. CD274

    Plasmid

    #107003

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mKikGR-C1

    Plasmid

    #54656

    Purpose

    Localization: C1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Habuchi et al PLoS One. 2008;3(12):e3944. doi: 10.1371

    Type

    Empty backbone

    Tags

    mKikGR

    Expression

    Mammalian

    Available Since

    June 11, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. dCas9-CBP core

    Plasmid

    #179543

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-FLAG-SF2

    Plasmid

    #99021

    Purpose

    Mammalian expression of SF2

    Depositor

    Honglin Chen

    Article

    Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

    Insert

    SF2 (SRSF1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-RAB10

    Plasmid

    #130883

    Purpose

    Expresses human RAB10 with N-terminal GFP tag

    Depositor

    Michael Bassik

    Article

    Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

    Insert

    RAB10 (RAB10 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTNS1

    Plasmid

    #64967

    Purpose

    Tn7 transposase expression

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    tnsABCD
    

    Available Since

    Oct. 23, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. Tet-off Full length Tau (0N4R, P301L/V337M)

    Plasmid

    #188573

    Purpose

    Dox-repressible expression of the 0N4R Tau isoform

    Depositor

    Franz-Ulrich Hartl

    Article

    Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

    Insert

    0N4R Tau (MAPT Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    P301L and V337M

    Promoter

    pTight TRE

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSFDuet-1_VNp15-mNeongreen

    Plasmid

    #182389

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-mNeongreen-His
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV_hSyn1_nLightG

    Plasmid

    #187179

    Purpose

    Expresses green norepinephrine indicator nLightG in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Has Service

    AAV9

    Insert

    nLightG
    

    Use

    AAV

    Tags

    Flag tag

    Promoter

    human Synapsin-1

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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