We narrowed to 41,926 results for: tro

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pcDNA3-FLAG-mTUT1

Plasmid

#60038

Purpose

Mammalian expression of FLAG-tagged mouse TUT1

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

TUT1 (Mtpap Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only
pET28a-λN-βLac

Plasmid

#162039

Purpose

Expresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)

Depositor

Karen Polizzi

Article

Moya Ramirez et al Biosens Bioelectron. 2019 Jul 15;137:199

Insert

λN domain of the λ phage and a β-lactamase fusion protein

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-GST-05230

Plasmid

#67874

Purpose

E.coli expression vector derived from pRSET encoding shy1 (um05230) with a N-terminal GST-fusion.

Depositor

Armin Djamei

Article

Rabe et al Mol Microbiol. 2013 Jul;89(1):179-88. do

Insert

shy1

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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PI(LM)-Rac1(WT)dc1.FLARE

Plasmid

#87590

Purpose

PI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
PBD
Rac1

Tags

YPet and dTurq

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mHH

Plasmid

#51057

Purpose

Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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TR-5'UTR-HP-GFP

Plasmid

#80594

Purpose

Expresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

GFP

Use

AAV

Expression

Mammalian

Mutation

Inserted 30 nt hairpin which is targeted by Csy4 …

Promoter

CBA

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSR28

Plasmid

#69253

Purpose

phlh-8::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

hlh-8

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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BC-A1-014

Plasmid

#78702

Purpose

A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator

Use

Synthetic Biology

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pFA-eCA

Plasmid

#133010

Purpose

pFA carrying a E. coli canA gene (Uniprot: P61517)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

Can (can E. coli)

Tags

6xhis

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

Plasmid

#133344

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_cpaA

Use

CRISPR

Promoter

constitutive

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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TU#1450_unc-53a_cDNA

Plasmid

#82361

Purpose

express unc-53a cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-53 a isoform (unc-53 Nematode)

Expression

Worm

Promoter

none

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK-kinase10

Plasmid

#59123

Purpose

FAK fret control sensor without FERM domain with 10 nm linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-Lifeact-7

Plasmid

#54846

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Lifeact-PAmCherry-miniSOG

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
Gal4pBS4-Pleum

Plasmid

#60329

Purpose

Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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FCIV1-GR S155A

Plasmid

#72702

Purpose

Lentiviral expression of GR Phospho-deficient mutant (2nd generation)

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Mutation

S155A

Promoter

ubiquitin

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
CRY2(delta2-6)-mCherry

Plasmid

#104182

Purpose

Expresses fusion of CRY2(delta aa 2-6) with mCherry

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2(delta2-6)-mCherry

Expression

Mammalian

Mutation

CRY2PHR with deletion of aa 2-6

Promoter

CMV

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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CRY2(neutral2-6)-mCherry

Plasmid

#104181

Purpose

Expresses fusion of CRY2(neutral aa 2-6) with mCherry

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2(neutral2-6)-mCherry

Expression

Mammalian

Mutation

CRY2PHR K2S M3S D4G K5S K6S

Promoter

CMV

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSetd2#2/Cre

Plasmid

#89650

Purpose

Expresses a Setd2-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Setd2 (Setd2 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG1

Plasmid

#166846

Purpose

Expresses Atg1 with ATG1 3'UTR in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Promoter

ATG1 Promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFS217-USP44_MSCV-TAP

Plasmid

#43896

Purpose

Retroviral expression of USP44 in mammalian cells

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Retroviral

Tags

FLAG, HA, and HIS

Expression

Mammalian

Available Since

May 6, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-Chronos(M140T)-mRuby2-ST

Plasmid

#109129

Purpose

Modified cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

Chronos(M140T)-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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MYMH 464

Plasmid

#138068

Purpose

Natural Terminator ECK120033736 inserted between mCherry and GFP

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23104-mCherry-33736-GFP-pSEVA-331Bb

Expression

Bacterial

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pDM3193

Plasmid

#113390

Purpose

Alpha factor inducible Ty3 with his3AI

Depositor

Suzanne Sandmeyer

Article

Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

Insert

Ty3-his3AI

Tags

his3AI

Expression

Yeast

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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UAS-akkordion dNhe

Plasmid

#41976

Depositor

Norbert Perrimon

Article

Hacker et al Genes Dev. 1998 Jan 15;12(2):274-84.

Insert

DRhoGEF2 (RhoGEF2 Fly)

Expression

Insect

Promoter

Hsp70

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only
pDEST-Hemmar-FLP(D)

Plasmid

#119895

Purpose

FLP(D) with a hsp70 promoter

Depositor

Rita Sousa-Nunes

Article

Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

Insert

FLP(D)

Use

Drosophila expression

Mutation

mutated form of FLP, which at position 5 contains…

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
907K

Plasmid

#160236

Purpose

FlightN-PipR-VP16-Sv40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

pristinamycin repressor
Flightin promoter

Tags

VP16

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 146a-Dendra2

Plasmid

#97113

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 146a Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 199-3-Dendra2

Plasmid

#97119

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 199 3 Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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GWF303

Plasmid

#133611

Purpose

CD95-1-2xPP7, NLS-PCP-DNCR2-P2a-BFP

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

CD95-1-2xPP7, PCP-DNCR2, BFP

Expression

Mammalian

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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GWF314

Plasmid

#133610

Purpose

CXCR4-com, NLS-com-GNCR1-P2a-BFP

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

CXCR4-6-com, com-GNCR1, BFP

Expression

Mammalian

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJBL712

Plasmid

#140382

Purpose

Expresses OtrR-regulated 3WJdB RNA aptamer driven by T7 promoter with the otrO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-otrO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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VV228: sdChR(C138S E154A)-TS in fck

Plasmid

#58523

Purpose

Expresses sdChR(C138S E154A) in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S, E154A)-TS

Use

Lentiviral

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine; changed Glutamate…

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pET20b-CeNLuc-6xHis

Plasmid

#135933

Purpose

Expresses the CeNLuc LumiFluor in bacterial cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

CeNLuc LumiFluor

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMT Draper-ITAM only-GFP

Plasmid

#126656

Purpose

Inducible expression of Draper-ITAM only-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper with Cytoplasmic ITAM residues Tyr all others mutated to Phe

Expression

Insect

Mutation

Cytoplasmic ITAM residues Tyr all others mutated …

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Snail1-3'UTR

Plasmid

#61781

Purpose

Luciferase reporter containing the 3'UTR of mouse Snail1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Snail1 3'UTR

Use

Luciferase

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
CALCOCO1 V142A-mycDDK

Plasmid

#137768

Purpose

CALCOCO1 CLIR mutant

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

CALCOCO1 V142A

Tags

mycDDK

Expression

Mammalian

Mutation

V142A

Promoter

CMV

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-ADA add back mutant pCMV5

Plasmid

#17554

Depositor

Domenico Accili

Article

Kitamura et al J Clin Invest. 2007 Sep . 117(9):2477-85

Insert

FLAG-FoxO1-ADA

Tags

FLAG

Expression

Mammalian

Mutation

siRNA-resistant Foxo1 by replacing 3 residues (un…

Available Since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only
pYC25

Plasmid

#63914

Purpose

Recipient overexpressing vector to construct YFP tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

YFP

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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Cav1-miniSOG

Plasmid

#67667

Purpose

Mammalian expression vector driving dog Caveolin1-minSOG

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

Dog Cav1-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
As5 (pBW414J105-arsR)

Plasmid

#123162

Purpose

Arsenic sensor used in the sensor cell array. pSB3K3 carrying J105-30arsR-t-ParsR-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J105-30arsR-t-ParsR-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J105, ParsR

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 41,926 results for: tro