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pX458-sgRNA_Ago1_2

Plasmid

#73534

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
Cntn2-AP-His

Plasmid

#71940

Purpose

Expresses the extracellular region of the Contactin 2 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn2 (Cntn2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCI-MOR205-1

Plasmid

#22361

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

MOR205-1 (Olfr221 Mouse)

Tags

Rho

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pCI-MOR260-1

Plasmid

#22372

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

MOR260-1 (Olfr715 Mouse)

Tags

Rho

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pREPROII-attB

Plasmid

#86557

Purpose

Dox inducible expression of reprogramming factors (Klf4, Sox2, Oct4, & cMyc), integration via phiC31 attB sites, excision via Cre/loxP

Depositor

Ralf Kuehn

Article

Merkl et al PLoS One. 2013;8(1):e55170. doi: 10.1371

Inserts

Oct4 (Pou5f1 Mouse)
cMyc (Myc Mouse)
Sox2 (Sox2 Mouse)
Kruppel-like factor 4 (Klf4 Mouse)

Use

Cre/Lox

Expression

Mammalian

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7b2

Plasmid

#14623

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7b2 (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
Luc-Sox2-3'UTR

Plasmid

#63895

Purpose

Luciferase reporter of mouse wildtype Sox2 3'UTR

Depositor

Lin He

Article

Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

Insert

SRY (sex determining region Y)-box 2 (Sox2 Mouse)

Expression

Mammalian

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only
Bin1 N-BAR domain

Plasmid

#168280

Purpose

Express N-BAR domain of Bin1 (aa 1-267) in pEGFP-N1 vector

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

Bin1 (Bin1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-267

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE flag M33

Plasmid

#1951

Depositor

Robert Kingston

Article

Levine et al Mol Cell Biol 2002 Sep;22(17):6070-8.

Insert

M33 (Cbx2 Mouse)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-SUN1 EGFP

Plasmid

#187675

Purpose

In vivo visualization of SUN1

Depositor

Mary Beckerle

Insert

SUN1 (Sun1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSCALPS-Flag-Mili-EGFP

Plasmid

#212576

Purpose

Lentiviral expression of Flag-tagged mouse Mili in mammalian cells

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

Mili (Piwil2 Mouse)

Use

Lentiviral

Tags

5x Flag and SNAP tag

Expression

Mammalian

Promoter

SFFV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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PB-Lmo2

Plasmid

#60666

Purpose

PiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Lmo2 (Lmo2 Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-c-Myc-V394D

Plasmid

#50775

Purpose

retroviral expression of mouse c-Myc V394D mutant

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

c-Myc V394D (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

V394D (does not bind to Miz-1)

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pBS-MK2

Plasmid

#29637

Depositor

Bruce Tempel

Insert

MK2 (Kcna2 Mouse)

Expression

Bacterial

Available Since

May 5, 2011

Availability

Academic Institutions and Nonprofits only
pES.HSV2.3a

Plasmid

#69593

Purpose

TCR a chain construct for generation of gBT-I mice

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR alpha chain cDNA from clone HSV2.3 (Tcra Mouse)

Expression

Mammalian

Promoter

H-2Kb

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only
pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP

Plasmid

#114729

Purpose

3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc gene

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

sgRNA M5, sgRNA M7, sgRNA M9

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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mEmerald-MKS1-N-18

Plasmid

#54183

Purpose

Localization: Cilium, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

MKS1

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBS-Foxd3 in situ

Plasmid

#37098

Depositor

Patricia Labosky

Article

Labosky et al Mech Dev. 1998 Aug;76(1-2):185-90.

Insert

Foxd3 (Foxd3 Mouse)

Use

In situ hybridization probe

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Hmga2 tr

Plasmid

#14793

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Hmga2 truncated (Hmga2 Mouse)

Expression

Mammalian

Mutation

truncataed HMGA2. As in human tumors, Hmga2-tr la…

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pMCP-MU-Luc (MCP-1 mut promoter in pGL3-basic)

Plasmid

#40325

Depositor

Alexander Dent

Article

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

MCP-1 promoter (mutated) (Ccl2 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

contains MCP-1 promoter region through the nucleo…

Promoter

MCP-1

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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