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pLPC-Flag-CHES1L-dFN

Plasmid

#51842

Purpose

Flag-CHES1L mutant lacking the forkhead binding site and N-terminal domains

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag

Mutation

contains CHES1 amino acids 206-490

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES1L-DBD-NLS

Plasmid

#51841

Purpose

Flag-CHES1L mutant encoding the DNA binding and NLS domains only

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag and NLS

Mutation

contains CHES1 amino acids 110-205

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES1L-dC-NLS

Plasmid

#51840

Purpose

Flag-CHES1L mutant lacking the C-terminal, with remaining NLS

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag and NLS

Mutation

contains CHES1 amino acids 1-205

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES1LdFC-NLS

Plasmid

#51838

Purpose

Flag-CHES1L mutant lacking the forkhead binding site and C-terminal domain, with remaining NLS

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag and NLS

Mutation

contains CHES1 amino acids 1-109

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES11L-dFC

Plasmid

#51837

Purpose

Flag-CHES1L mutant lacking the forkhead binding site and the C-terminal domains

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag

Mutation

contains CHES1 amino acids 1-109

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
TRS6E1b-luc(-732/-721) mutant 4

Plasmid

#45387

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI-1 promoter -732/-721 four point mutation (SERPINE1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

mutated from agacaaggttgt to acactaggatga

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
Hoxb6-3'UTR

Plasmid

#31523

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

Hoxb6-3'UTR (Hoxb6 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
A1-LCD WT

Plasmid

#169713

Purpose

Bacterial expression of human hnRNPA1-A Low complexity domain including amino acids 186-320

Depositor

Tanja Mittag

Article

Martin et al Science. 2020 Feb 7;367(6478):694-699. d

Insert

hnRNPA1 (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

hnRNPA1-A Low complexity domain including amino a…

Promoter

T7

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pIG-601_NY-ESO-1_TCR_GFP

Plasmid

#207482

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

GFP, NY-ESO-1_TCR

Use

CRISPR

Mutation

Potential silent point mutation in GFP sequence

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-hWIPI4-3×FLAG

Plasmid

#215512

Purpose

Expresses FLAG tagged human WIPI4.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

WD-repeat protein interacting with phosphoinositides 4 (WDR45 Human)

Use

Retroviral

Tags

3×FLAG

Expression

Mammalian

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau P301L

Plasmid

#46908

Purpose

expresses EGFP tagged Tau P301L in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau P301L (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

P301L

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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PB-TO-NFIA.1-SOX9

Plasmid

#182310

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

NFIA-T2A-SOX9 (NFIA, SOX9 Human)
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and TRE3G

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)

Plasmid

#197892

Purpose

Can be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

hM4D/2A/GFP

Use

AAV

Mutation

N/A

Promoter

EF1a

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pKK-BiFC-Venus

Plasmid

#105804

Purpose

Concomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

VN173 (I152L); VC155

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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PGL4-4X TE Down (GQs) Renilla

Plasmid

#70039

Purpose

Plasmid for testing 5’UTR TE Down (G-quadruplex) folding elements

Depositor

Hans-Guido Wendel

Article

Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

Insert

4x TE Down (G-quadruplexes) in 5'UTR

Use

Luciferase

Tags

renilla luciferase

Expression

Mammalian

Mutation

4 repeats of TE down motif (G-quadruplexes) in 5&…

Promoter

SV40

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
pAJE3-E7

Plasmid

#214359

Purpose

Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

Depositor

Ryan Mehl

Article

Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

Inserts

2nd generation Tet2 "E7" aminoacyl tRNA synthetase
Lpp promoted M. jannaschii tRNA
Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
Orthogonal "D4II" ColE1 origin

Expression

Bacterial

Promoter

AmpR and lpp

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Bst LF-6xHis

Plasmid

#159148

Purpose

expresses His-tagged large fragment of Bst polymerase

Depositor

Andrea Pauli

Article

Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

Insert

large fragment of Geobacillus stearothermophilus DNA polymerase I

Tags

His

Expression

Bacterial

Available Since

Sept. 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Proinsulin-NanoLuc in pLX304

Plasmid

#62057

Purpose

Luminescent reporter of insulin secretion

Depositor

David Altshuler

Article

Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

Insert

Proinsulin-NanoLuc (Ins2 Mouse, Synthetic)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

Inserted NanoLuc into C-peptide of mouse Ins2 cod…

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pTetOFF SNCA-A53T

Plasmid

#215372

Purpose

All-in-one lentiviral construct for the dox repressible expression of the A53T mutant of alpha-synuclein.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

SNCA (SNCA Human)

Expression

Mammalian

Mutation

Ala53Thr

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pX026

Plasmid

#167153

Purpose

MoClo-compatible Level 1 (position 4) vector encoding Neonothopanus nambi luciferase nnLuz with C-terminal His-tag under control of 0.4 kb 35S promoter, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

p35s-nnLuz-ocsT

Use

Luciferase

Tags

His6-tag

Expression

Plant

Promoter

p35s

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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PB-TO-NFIA.1-SOX9 (bi-directional promoter)

Plasmid

#182306

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

NFIA (NFIA Human)
SOX9 (SOX9 Human)
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

TRE3G-Bi-directional

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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MAC-TFRC

Plasmid

#172420

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Transferrin receptor protein 1 (TfR) (CD antigen CD71) (TFRC Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-EF1a-FLuc-WPRE-HGHpA

Plasmid

#87951

Purpose

Recombinant single-stranded AAV transfer vector expressing Firefly Luciferase under the EF1a promoter

Depositor

Mark Kay

Article

Paulk et al Mol Ther. 2017 Sep 25. pii: S1525-0016(1

Insert

Firefly Luciferase

Use

AAV

Promoter

EF1a

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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lenti-HA-mBec1 F121A

Plasmid

#99506

Purpose

Lentiviral expression of mutant Becn1 leading to hyperactive autophagy

Depositor

Congcong He

Article

Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

Insert

Beclin 1 (Becn1 Mouse)

Use

Lentiviral

Tags

HA

Mutation

F121A

Promoter

CMV

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-his-3C_Tau352

Plasmid

#100092

Purpose

Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

Depositor

Nikolai Sluchanko

Article

Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

Insert

Fetal microtubule-associated protein tau (MAPT Human)

Tags

His6

Expression

Bacterial

Mutation

wild type sequence

Promoter

T7

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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METTL3 shRNA 1 tet pLKO puro

Plasmid

#162985

Purpose

Tet-inducible shRNA targeting human METTL3 #1

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

methyltransferase like 3 (METTL3 Human)

Use

Lentiviral

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-SNCA-A53T-mRuby3

Plasmid

#215379

Purpose

Expression of the A53T mutant of alpha-synuclein with a C-terminal mRuby3 tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

SNCA (SNCA Human)

Tags

mRuby3 tag

Expression

Mammalian

Mutation

Ala53Thr

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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METTL3 shRNA 2 tet pLKO puro

Plasmid

#162984

Purpose

Tet-inducible shRNA targeting human METTL3 #2

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

methyltransferase like 3 (METTL3 Human)

Use

Lentiviral

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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MAC-DPP4

Plasmid

#172416

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Dipeptidyl peptidase 4 (DPP4 Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

Plasmid

#89991

Purpose

Donor template for generation of SOX2-ntdTomato reporter cell lines

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

Insert

SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)

Use

CRISPR

Promoter

none

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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