We narrowed to 11,999 results for: SHA

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pMCSF-R(DD)-luc

Plasmid

#12424

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1994 Dec . 14(12):8085-95

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Mutation

Deletion from bp-86 to -37 (created a KpnI site i…

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pGEX4T3-GST-PolB(WT)

Plasmid

#177131

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(WT) with a TEV protease site located between the GST tag and PolB(WT)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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DHX58

Plasmid

#155542

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DHX58 (DHX58 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-ultraID-LMNB1

Plasmid

#207775

Purpose

Donor template for Blast-2A-ultraID insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

LMNB1 Homology Arms flanking a Blast-ultraID Cassette (LMNB1 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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fck-Jaws-KGC-GFP-ER2

Plasmid

#65012

Purpose

Lentivirus-mediated expression of Jaws under the CaMKII promoter.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-GFP-ER2

Use

Lentiviral

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

CaMKII

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Gli3_Anti-Sense

Plasmid

#124440

Purpose

Plasmid for anti-sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

GLI-Kruppel family member GLI3 (Gli3 Mouse)

Use

In situ probe

Promoter

T7

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR4-hDND1

Plasmid

#70070

Purpose

Gateway entry vector for human DND1 with stop codon

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (DND1 Human)

Use

Gateway entry vector

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF692

Plasmid

#88701

Purpose

Donor Vector containing ZNF692 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF692 (ZNF692 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgKEAP1-1

Plasmid

#74985

Purpose

sgKEAP1-1, puromycin selection

Depositor

William Hahn

Article

Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

Insert

KEAP1 (KEAP1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v3 IQSEC1 WT

Plasmid

#162018

Purpose

Expression of IQSEC1 splice variant 3

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-v4 IQSEC1 WT

Plasmid

#162019

Purpose

Expression of IQSEC1 splice variant 4

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pD2_miniluc_newP7

Plasmid

#174105

Purpose

adding the mini luciferase and mini promoter to the MPRA lenti construct

Depositor

Paul Khavari

Article

Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

Insert

mini luciferase mini-promoter

Expression

Bacterial

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-TRE3G_3xFLAG-PGC1a4_CMV-mCherry:T2A:Hygro

Plasmid

#154263

Purpose

Dox/Tet-inducible expression of the Human PGC-1a4 splice variant in mammalian cells.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

3xFLAG-PGC-1a4 splice variant (PPARGC1A Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE3G

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCriprV2-sgRNA-PER1-#1

Plasmid

#189987

Purpose

Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Period1 (PER1 Human)

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pINDUCER20 MEF2C S222D

Plasmid

#107234

Purpose

MEF2C phosphomimetic mutant serine to aspartic acid

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C

Use

Lentiviral

Expression

Mammalian

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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DHX37

Plasmid

#155539

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DHX37 (DHX37 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P2-MDMX (1-120)

Plasmid

#62065

Purpose

Expression of human MDM2 (1-120) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

MDM4 (MDM4 Human)

Tags

GST

Expression

Bacterial

Mutation

contains residues 1-120

Promoter

tac

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#1

Plasmid

#208387

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#1 gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgZBP1 (Zbp1 Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#2

Plasmid

#208388

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#2 gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgZBP1 (Zbp1 Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-moxGFP-Puro-MAPRE1

Plasmid

#207794

Purpose

Donor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

MAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Dec. 1, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-ZNF770

Plasmid

#88562

Purpose

Donor Vector containing ZNF770 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF770 (ZNF770 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-mScarlet-ACTB

Plasmid

#207754

Purpose

Donor template for Puro-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

ACTB Homology Arms flanking a Puro-mScarlet Cassette (ACTB Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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FIP1L1

Plasmid

#155605

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

FIP1L1 (FIP1L1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized

Plasmid

#166834

Purpose

Expression and purification of NLRP1 (without the PYD and CARD) from mammalian cells

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

TEV-GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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Clover-LaminA-C-18

Plasmid

#56370

Purpose

Localization: Nuclear Envelope, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

LaminA (LMNA Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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HT115_pAAV_hSyn-DiO-SomQuasAr6b_EGFP-P2A-somCheRiff_HA

Plasmid

#178827

Purpose

Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

SomQuasAr6a_EGFP-P2A-somCheRiff_HA

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP, HA

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF768

Plasmid

#88524

Purpose

Donor Vector containing ZNF768 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF768 (ZNF768 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
H14-MBP-SUMO-FLAG-LEM

Plasmid

#101778

Purpose

Expresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

MAN1 (LEMD3 Human)

Tags

His14-MBP-SUMO-FLAG

Expression

Bacterial

Mutation

amino acids 6-51 of human MAN1 (LEM domain)

Promoter

T5

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pRRLNeo-pEF1a-p53ashL344P-mCerulean

Plasmid

#69581

Purpose

Expresses p53-L344P mutant tagged with mCerulean

Depositor

Galit Lahav

Article

Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

Insert

p53-L344P (TP53 Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mammalian

Mutation

p53 L344P mutation that prevents oliogomerization…

Promoter

EF1alpha

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX301-ARF6-T157N-TagRFP

Plasmid

#162030

Purpose

Fast cycling ARF mutant

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF6 (ARF6 Human)

Use

Lentiviral

Mutation

T151N

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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