We narrowed to 59,496 results for: tra
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Plasmid#126911PurposePlasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiaeDepositorInsertX-4 and XII-5 gRNA
UseCRISPRAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVH016
Plasmid#80400PurposeNo anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).DepositorUseSynthetic BiologyTagsC-terminal 3xFLAG tag and C-terminal LZx domainExpressionBacterialAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ496
Plasmid#44488DepositorInsert500bp Mos1, unc-119, fosmid cassette
UseFosmid recombineering cassetteAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pR540
Plasmid#207885Purposetemplate for 2 gRNA amplificationDepositorInsertscaffold-pU6
ExpressionBacterialAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS021
Plasmid#207917PurposeLevel 0 for gRNA in E2 module (5' CCAT-3' ACTA)DepositorInsertpU6-AarI-scaffold-term
UseCRISPRAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKE013
Plasmid#207916PurposeLevel 0 for gRNA in E1 module (5' CTGC-3' CCAT)DepositorInsertpU3-AarI-scaffold-term
UseCRISPRAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAL725
Plasmid#119862Purposeprecusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287CDepositorInsertD-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
TagsnoneExpressionBacterialMutationLeucine 244 to Cysteine, Aspartate 287 to CysteinePromoterT5Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1002
Plasmid#44477DepositorInsertMinimal Mos1, NeoR, peel-1, [4-3] Gateway
UseGateway destination vectorAvailable SinceMay 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP
Plasmid#194934PurposeLV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(Y186F/Y229A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPI420
Plasmid#113230Purposesafe haven knock-in plasmid for the act5 locus, Expression of H2B-mCherryDepositorInsertH2B
UseSafe haven knock-in plasmid for the act5 locus, h…TagsmCherryPromoteract5Available SinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-2_PolyU(1)_Tornado-Corn
Plasmid#159487PurposeTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination Module:Linear-2_PolyU(1)
ExpressionMammalianPromoterU6-27Available SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDM1516
Plasmid#109001Purposesafe haven knock-in plasmid for the act5 locus, C-terminal mCherry taggingDepositorTypeEmpty backboneUseSafe haven knock-in plasmid for the act5 locus, c…TagsmCherryAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1117 - [4-1] - Promoter - Psmu-1(no_atg)
Plasmid#159886PurposeThree-fragment Gateway compatible promoter with PATCs for germline expression from arraysDepositorInsert[4-1] - Promoter - Psmu-1(no_atg)
ExpressionWormMutationNot applicableAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start)
Plasmid#159887PurposeThree-fragment Gateway compatible promoter with PATCs for germline expression from arraysDepositorInsert[4-1] - Promoter - Psmu-2 (no_start)
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP7
Plasmid#71470PurposeEncodes human full length SENP7. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 7 (SENP7 Human)
TagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available SinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK357
Plasmid#71568PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
ExpressionBacterialPromoterT7Available SinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_M7NS_AmpR
Plasmid#119157PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
UseCrisprPromoterJ23119Available SinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_M2S_AmpR
Plasmid#119154PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region
UseCrisprPromoterJ23119Available SinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1359 - Intron GG2 - smu-1
Plasmid#159805PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG2 - smu-1
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ907
Plasmid#44473DepositorInsertMinimal Mos1, NeoR, [4-3] Gateway
UseGateway destination vectorAvailable SinceApril 25, 2013AvailabilityAcademic Institutions and Nonprofits only