We narrowed to 1,891 results for: ogn

1. SETD2(SET)-Cas9

   Plasmid

   #186700

   Purpose

   Plasmid encoding SETD2-Cas9 fusion under CMV promoter

   Depositor

   Jennifer Doudna

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

   Insert

   SETD2(SET)-Cas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_PTPN22_Y-phosphatase

   Plasmid

   #110048

   Purpose

   Protein expression and purification of PTPN22_Y-phosphatase

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PTPN22_Y-phosphatase (PTPN22 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINS-AREL1

   Plasmid

   #66710

   Purpose

   Expresses AREL1 HECT domain in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Insert

   KIAA0317 (AREL1 )
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pDEST17-ScChd1(118-1274)

   Plasmid

   #221207

   Purpose

   ScChd1-aa118-1274 chromatin remodeler

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   ScChd1 (aa118-1274)
   

   Use

   Tags

   6xHis-tag (Precission protease cleavable)

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-117 and 1275-1468

   Promoter

   Available since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_NR2F2_Hormone-recep

   Plasmid

   #109826

   Purpose

   Protein expression and purification of NR2F2_Hormone-recep

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   NR2F2_Hormone-recep (NR2F2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cathepsin S

   Plasmid

   #84694

   Purpose

   for expression of Cathepsin S in Leishmania tarantolae

   Depositor

   Hans Brandstetter

   Article

   Freier et al Sci Rep. 2015 Aug 3;5:12707. doi: 10.103

   Insert

   cathepsin S (CTSS Human)
   

   Use

   Expression in leishmania tarantolae

   Tags

   his-tag

   Expression

   Mutation

   Promoter

   unknown

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. CD3Zeta-GFP

   Plasmid

   #38298

   Depositor

   Matthew Krummel

   Article

   Krummel et al Science. 2000 Aug 25;289(5483):1349-52.

   Insert

   CD3Zeta (Cd247 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pACT2-μ3A

   Plasmid

   #198174

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-3 μ3A
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   silent substitution in codon 225

   Promoter

   ADH1

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACT2-μ3B

   Plasmid

   #198175

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-3 μ3B
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. p1553 CMV-EBNA1 (delta GGA)

    Plasmid

    #26648

    Depositor

    Bill Sugden

    Article

    Aiyar et al J Biol Chem. 1998 Dec 4. 273(49):33073-8

    Insert

    Epstein-Barr Nuclear Antigen 1 (EBNA-1 Epstein-Barr Virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of 224 amino acids of EBNA-1 (aa101-324 …

    Promoter

    Available since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_YWHAZ_14-3-3

    Plasmid

    #109808

    Purpose

    Protein expression and purification of YWHAZ_14-3-3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    YWHAZ_14-3-3 (YWHAZ Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDOC-GG

    Plasmid

    #149377

    Purpose

    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

    Depositor

    Mark Pallen

    Article

    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

    Inserts

    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ntn4-AP-His

    Plasmid

    #71980

    Purpose

    Expresses the entire Netrin 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntn4 (Ntn4 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSP2262

    Plasmid

    #70703

    Purpose

    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    D10A & H557A in SaCas9

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103_PDCD6_EF-hand

    Plasmid

    #110009

    Purpose

    Protein expression and purification of PDCD6_EF-hand

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PDCD6_EF-hand (PDCD6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHH0103_PIKFYVE_DEP

    Plasmid

    #109789

    Purpose

    Protein expression and purification of PIKFYVE_DEP

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PIKFYVE_DEP (PIKFYVE Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET14b PHAS-I

    Plasmid

    #15679

    Depositor

    John Lawrence

    Article

    Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

    Insert

    PHAS-I (Eif4ebp1 Rat)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Nucleotides 1-354 of rat PHAS-I plus 450bp of 3&#…

    Promoter

    Available since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pOPINK-TRABID (NZF1, aa 1-33)

    Plasmid

    #65437

    Purpose

    Expresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Insert

    TRABID (ZRANB1 Human)
    

    Use

    Tags

    His6-GST

    Expression

    Bacterial

    Mutation

    only codes for amino acids 1-33 of TRABID, which …

    Promoter

    T7

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

19. EGFP-UTRN-mini

    Plasmid

    #158749

    Purpose

    Expresses a shorter version of Utrophin (amino acid 35-136) with amino terminus eGFP in mammalian cells

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

    Insert

    UTRN-mini
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28b-NmCas9-Flag

    Plasmid

    #109030

    Purpose

    Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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