2,225 results

1. pCAG-2dV-Camui (T286A)

   Plasmid

   #220367

   Purpose

   T286A phosphodead mutant of pCAG-2dV-Camui

   Depositor

   Ryohei Yasuda

   Article

   Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

   Insert

   mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T286A)-mEGFP(A206K) (Camk2a Synthetic, Rat)
   

   Use

   Tags

   mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Alanine

   Promoter

   CAG

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. ChR2-YFP-NavII-III

   Plasmid

   #26057

   Depositor

   Matthew Grubb

   Article

   Grubb et al PLoS One. 2010 . 5(10):e13761.

   Insert

   NavII-III
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

3. mCherry-CathespinB-6

   Plasmid

   #55007

   Purpose

   Localization: Lysosomes, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   CathespinB
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pEGFP-C1-CaMKIIbeta(A303R)

   Plasmid

   #21224

   Depositor

   Tobias Meyer

   Article

   Shen et al Science. 1999 Apr 2. 284(5411):162-6.

   Insert

   CaMKIIbeta(A303R) (Camk2b Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CaMKIIbeta(A303R) (Lab plasmid CF 0002)

   Promoter

   Available since

   July 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. mTagRFP-T-Cx43-7

   Plasmid

   #58009

   Purpose

   Localization: Gap Junctions, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Cx43
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pEF Doc2beta WT GFP

   Plasmid

   #128820

   Purpose

   To visualize Doc2beta WT in PC12 cells

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2beta (Doc2b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHes1-GFP (CC#109)

   Plasmid

   #15133

   Depositor

   Connie Cepko

   Article

   Furukawa et al Neuron. 2000 May . 26(2):383-94.

   Insert

   Hes1 (Hes1 Rat)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pORANGE Shank1-GFP KI

   Plasmid

   #131500

   Purpose

   Endogenous tagging of Shank1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Shank1, Shank1 Mouse, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-syn-FLEX-synaptoPAC-minWPRE

   Plasmid

   #153100

   Purpose

   Cre-conditional expression of synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

   Depositor

   Dietmar Schmitz

   Article

   Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

   Inserts

   bPAC(WT)
   Synaptophysin (Syp Rat)
   

   Use

   AAV

   Tags

   His tag, mScarlet, and myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 HA-rnSOX9 ΔC (1-381)

    Plasmid

    #62973

    Purpose

    Expresses HA-tagged rat SOX9 residues 1-383

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    SOX9 (Sox9 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codes for residues 1-381

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1 HA-rnSOX9 PQA/S (286-507)

    Plasmid

    #62975

    Purpose

    Expresses HA-tagged rat SOX9 residues 286-507

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    SOX9 (Sox9 Rat)
    

    Use

    Tags

    HA (YPYDVPDY)

    Expression

    Mammalian

    Mutation

    codes for residues 286-507

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCLX-RIP-mCherry

    Plasmid

    #114310

    Purpose

    Tissue-specific expression of the mCherry living color under the control of the Rat Insulin 2 promoter (RIP)

    Depositor

    Patrick Salmon

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mEos2-Cx43-7

    Plasmid

    #57365

    Purpose

    Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Cx43
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pEGFP-Sec22b-shR

    Plasmid

    #208358

    Purpose

    Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b

    Depositor

    Paula Nunes-Hasler

    Article

    Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

    Insert

    Sec22b (Sec22b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    four silent mutations introduced to render shRNA …

    Promoter

    CMV

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET30b jGCaMP8f F366H

    Plasmid

    #204139

    Purpose

    Production of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coli

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f F366H
    

    Use

    Tags

    N-terminal His tag

    Expression

    Bacterial

    Mutation

    Phenylalanine 366 changed to histidine

    Promoter

    T7

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T-1 3xFlag-ERK2

    Plasmid

    #47573

    Purpose

    Bacterial expression plasmid for GST-3xFlag-tagged ERK2

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    3xFlag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

    Plasmid

    #68720

    Purpose

    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

    Depositor

    Mark Huebener, Tobias Bonhoeffer, Tobias Rose

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Has Service

    AAV1

    Insert

    mRuby2-P2A-GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Cre-dependent expression from inverted open readi…

    Promoter

    hSyn1-FLEX

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pORANGE Rims2-GFP KI

    Plasmid

    #131495

    Purpose

    Endogenous tagging of RIM2: C-terminal (amino acid position: S1551)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Rims2, Rims2 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CaV1.3e[8a,11,31b,Δ32,42a] mut

    Plasmid

    #26576

    Depositor

    Diane Lipscombe

    Article

    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

    Insert

    CACNA1D (Cacna1d Rat)
    

    Use

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Contains exons 8a, 11, 31b and 42a.Deletion of ex…

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

20. mCherry-RnBarr2

    Plasmid

    #137800

    Purpose

    Visualization of beta-arrestin2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFP-GLT1b

    Plasmid

    #162516

    Purpose

    Rat GLT-1b cDNA N-terminally tagged with EGFP

    Depositor

    Josef Kittler

    Article

    Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

    Insert

    GLT1b (Slc1a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. lentiviral shRNA GEF-H1

    Plasmid

    #21477

    Depositor

    Richard Huganir

    Article

    Kang et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

    Insert

    rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2 Rat)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

23. pORANGE GFP-CACNA1E KI

    Plasmid

    #131481

    Purpose

    Endogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cacna1e, Cacna1e Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGP-CMV-GCaMP3-373

    Plasmid

    #91779

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    GENIE Project, Douglas Kim

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP3-373
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. H6-AP180

    Plasmid

    #63006

    Purpose

    expresses the adaptor protein AP180 in bacteria

    Depositor

    Ernst Ungewickell

    Article

    Norris et al J Biol Chem. 1995 Jan 6;270(1):214-7.

    Insert

    AP180 (Snap91 Rat)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

26. TH mAb_BCN53.1.1D10_HC_Rat IgG2a

    Plasmid

    #182898

    Purpose

    Mammalian expression of TH rat mAb HC gene

    Depositor

    Heng Zhu

    Insert

    anti-TH (human) light chain (TH Rat)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TH mAb_BCN53.1.1D10_LC_Rat Ig Kappa

    Plasmid

    #182899

    Purpose

    Mammalian expression of TH rat mAb LC gene

    Depositor

    Heng Zhu

    Insert

    anti-TH (human) light chain (TH Rat)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV hSyn syph-GFP-myc-BoNT/B(1-146)-iLID

    Plasmid

    #122981

    Purpose

    AAV plasmid with human synapsin promoter driving synaptophysin fused to GFP, iLID(V416I) and BoNT/B amino acids 1-146. Co-express with SSPB-BoNT(147-441, Y365A) for vPA-BoNT

    Depositor

    Matthew Kennedy

    Article

    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

    Insert

    Syph-GFP-myc-BoNT/B(1-146)-iLID (Syp Synthetic, Rat)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLVX-SV40-Galpha-i3 ONE-GO

    Plasmid

    #204329

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    SV40 Galpha-i3 ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. G protein alpha-s-mCherry

    Plasmid

    #66968

    Purpose

    G protein alpha-s internally tagged with mCherry and EE epitope

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    G-alpha-s-EE-mCherry (Gnas Discosoma sp., Rat)
    

    Use

    Tags

    internal EE epitope (residues 189-194 in alpha- s…

    Expression

    Mammalian

    Mutation

    BamHI sites in the alpha-s cDNA were removed and …

    Promoter

    CMV

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge