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Showing: 1121 - 1160 of 3015 results

  1. VHACg/pDONR221

    Plasmid
    #172094
    Purpose
    Genomic fragment containing the upstream region and coding region of DET3/VHA-C cloned into pDONR221
    Depositor
    George Haughn
    Article
    Shi et al Plant Physiol. 2019 Nov;181(3):901-915.
    Insert
    VHA-C genomic region including 530bp upstream of the ATG, exons, introns, and no stop codon (DET3 Mustard Weed)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    530bp upstream of VHA-C ATG
    Available since
    Oct. 20, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    172094-plasmid-map-sequence-id-345740

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  7. pAN19-3xFLAG-ccvTIR1-NOSt

    Plasmid
    #108546
    Purpose
    Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator
    Depositor
    Keiko Torii
    Article
    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d
    Insert
    TRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
    Use
    Cloning vector
    Tags
    3xFLAG
    Expression
    Mutation
    Changed F79 to G
    Promoter
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108546-plasmid-map-sequence-id-210054

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  8. mitoTALECD for_atp1_1397CN

    Plasmid
    #186203
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186203-plasmid-map-sequence-id-368337

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  9. pBItetDT240GFP

    Plasmid
    #96904
    Purpose
    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 240 interrupted CTG repeats in exon 15 located
    Depositor
    Thomas Cooper
    Article
    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10
    Insert
    EGFP and human DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)
    Use
    Tetracycline responsive and bidirectional
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline responsive and bidirectional
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    96904-plasmid-map-sequence-id-193222

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  12. MT2

    Plasmid
    #223330
    Purpose
    Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    3HA-TurboID-EGFP-OMP25 (Synj2bp Rat)
    Use
    Tags
    3HA-TurboID-EGFP
    Expression
    Plant
    Mutation
    Promoter
    CaMV35S
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    223330-plasmid-map-sequence-id-455593

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  13. MT3

    Plasmid
    #223331
    Purpose
    Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    3HA-EGFP-miniTurboID-OMP25 (Synj2bp Rat)
    Use
    Tags
    3HA-EGFP-miniTurboID
    Expression
    Plant
    Mutation
    Promoter
    CaMV35S
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    223331-plasmid-map-sequence-id-455811

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  14. MT4

    Plasmid
    #223332
    Purpose
    Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Article
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    3HA-miniTurboID-EGFP-OMP25 (Synj2bp Rat)
    Use
    Tags
    3HA-miniTurboID-EGFP
    Expression
    Plant
    Mutation
    Promoter
    CaMV35S
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    223332-plasmid-map-sequence-id-455589

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  15. 1BR9-AtWUSSTOP-Δα3

    Plasmid
    #202056
    Purpose
    E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL with the third helix in the homeodomain deleted.
    Depositor
    Markita Landry
    Article
    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:
    Insert
    AtWUS E. Coli Codon Optimized, third helix of homeodomain deleted (WUS Mustard Weed)
    Use
    Tags
    6xHis and GFP11
    Expression
    Bacterial
    Mutation
    third helix of homeodomain deleted (AA82-102)
    Promoter
    T7 Promoter
    Available since
    Oct. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202056-plasmid-map-sequence-id-409953

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  16. mitoTALECD for_atp1_1333NC

    Plasmid
    #186202
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333N of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333C of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    186202-plasmid-map-sequence-id-380568

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  17. mitoTALECD for_atp1_1333CN

    Plasmid
    #186201
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333B of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186201-plasmid-map-sequence-id-368308

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  18. pAN19-TIR1-GUS-NOSt

    Plasmid
    #108547
    Purpose
    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator
    Depositor
    Keiko Torii
    Article
    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d
    Insert
    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    Use
    Cloning vector
    Tags
    GUS
    Expression
    Mutation
    Promoter
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108547-plasmid-map-sequence-id-210059

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  19. pAN19-ccvTIR1-GUS-NOSt

    Plasmid
    #108548
    Purpose
    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator
    Depositor
    Keiko Torii
    Article
    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d
    Insert
    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    Use
    Cloning vector
    Tags
    GUS
    Expression
    Mutation
    Changed F79 to G
    Promoter
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108548-plasmid-map-sequence-id-210051

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  21. pRL_GI_PCB28

    Plasmid
    #98745
    Purpose
    For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    heme oxygenase 1 (TED4 Mustard Weed)
    Phycocyanobilin:ferredoxin oxidoreductase
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    ADH1m and PGK1
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98745-plasmid-map-sequence-id-189854

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  23. pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid
    #234370
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs
    Depositor
    Michael Smanski
    Article
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Inserts
    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-13XGP41
    gRNA 1-1 and gRNA 1-2
    Use
    Synthetic Biology
    Tags
    GB1, GP41, and sfGFP
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234370-plasmid-map-sequence-id-469430

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  24. pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid
    #234372
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs
    Depositor
    Michael Smanski
    Article
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Inserts
    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-24XGP41
    gRNA 1-1 and gRNA 1-2
    Use
    Synthetic Biology
    Tags
    GB1, GP41, and sfGFP
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234372-plasmid-map-sequence-id-469423

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  25. mitoTALECD for_-20G_1333CN

    Plasmid
    #186200
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted 20th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186200-plasmid-map-sequence-id-368306

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  26. mitoTALECD for_ -13G_1397CN

    Plasmid
    #186199
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted -13th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186199-plasmid-map-sequence-id-368290

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  27. mitoTALECD for_ -6G_1333CN

    Plasmid
    #186198
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted -6th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186198-plasmid-map-sequence-id-368288

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  28. pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 4

    Plasmid
    #175815
    Purpose
    pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 4 (AT1G09270) without stop codon for C-terminal epitope tags
    Depositor
    Lennart Wirthmueller, Mark Banfield
    Article
    Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1
    Insert
    IMPORTIN ALPHA ISOFORM 4 (IMPA-4 Mustard Weed)
    Use
    Pentr plasmid for gateway cloning
    Tags
    none
    Expression
    Mutation
    Promoter
    none
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175815-plasmid-map-sequence-id-346165

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  29. pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 2

    Plasmid
    #175816
    Purpose
    pENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 2 (AT4G16143.1) without stop codon for C-terminal epitope tags
    Depositor
    Lennart Wirthmueller, Mark Banfield
    Article
    Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1
    Insert
    IMPORTIN ALPHA ISOFORM 2 (IMPA-2 Mustard Weed)
    Use
    Pentr plasmid for gateway cloning
    Tags
    none
    Expression
    Mutation
    natural polymorphism P65S, annotated in Genbank f…
    Promoter
    none
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175816-plasmid-map-sequence-id-346164

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  30. pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 9

    Plasmid
    #175817
    Purpose
    pENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 9 (AT5G03070.1) without stop codon for C-terminal epitope tags
    Depositor
    Lennart Wirthmueller, Mark Banfield
    Article
    Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1
    Insert
    IMPORTIN ALPHA ISOFORM 9 (IMPA-9 Mustard Weed)
    Use
    Pentr plasmid for gateway cloning
    Tags
    none
    Expression
    Mutation
    Promoter
    none
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175817-plasmid-map-sequence-id-346163

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  31. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71287-plasmid-map-sequence-id-352460

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  33. pHEE401

    Plasmid
    #71286
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…
    Available since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    71286-plasmid-map-sequence-id-127777

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  34. pMDC32B-AtTAS1c-D2-B/c-AtMIR173

    Plasmid
    #137885
    Purpose
    Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.
    Depositor
    Alberto Carbonell
    Article
    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583
    Inserts
    AtTAS1c-D2-B/c
    2x35S-AtMIR173-Tnos
    Use
    Tags
    Expression
    Plant
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Promoter
    Available since
    May 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    137885-plasmid-map-sequence-id-275856

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  35. pGem-sHyper:sAPX

    Plasmid
    #84738
    Purpose
    Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10
    Inserts
    sAPX (SAPX Mustard Weed)
    sHyper (HyPer2 targeted to nucleus)
    Use
    Tags
    nuclear localisation signals from the coding sequ…
    Expression
    Plant
    Mutation
    Promoter
    pFMV/p35S
    Available since
    July 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    84738-plasmid-map-sequence-id-180317

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  36. pGem-nHyPer:sAPX

    Plasmid
    #84735
    Purpose
    Simultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cells
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10
    Inserts
    sAPX (SAPX Mustard Weed)
    nHyper (HyPer2 targeted to nucleus)
    Use
    Tags
    nuclear localisation signals from the coding sequ…
    Expression
    Plant
    Mutation
    Promoter
    FMV/35S
    Available since
    July 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    84735-plasmid-map-sequence-id-180316

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  37. pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 1

    Plasmid
    #175814
    Purpose
    pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 1 (AT3G06720) without stop codon for C-terminal epitope tags
    Depositor
    Lennart Wirthmueller, Mark Banfield
    Article
    Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1
    Insert
    IMPORTIN ALPHA ISOFORM 1 (IMPA-1 Mustard Weed)
    Use
    Pentr plasmid for gateway cloning
    Tags
    none
    Expression
    Mutation
    Promoter
    none
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175814-plasmid-map-sequence-id-346174

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  38. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71288-plasmid-map-sequence-id-166343

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  39. pVZ_PcoaT_SQE1_LUP1

    Plasmid
    #103857
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and LUP1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    lupeol synthase 1 (LUP1 Mustard Weed)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    103857-plasmid-map-sequence-id-217123

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  40. pVZ_PcoaT_SQE1_THAS1

    Plasmid
    #103859
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and THAS1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    thalianol synthase 1 (THAS1 Mustard Weed)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    103859-plasmid-map-sequence-id-217087

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Showing: 1121 - 1160 of 3015 results