We narrowed to 6,273 results for: nls
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Plasmid#204718PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
ExpressionMammalianAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
MCS-T2a-GFPnls-ER-Cre-ER
Plasmid#200104PurposeVector For TwistDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Twist3cterm-GFPnls-T2A-ER-Cre-ER
Plasmid#200105DepositorInsertTwist3:cTer-KI-eGFPnls-T2A-ER-Cre-ER
UseUnspecifiedAvailable SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-6FLAG (no NLS)
Plasmid#193322PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..DepositorInsertIAA17
Tags6FLAGExpressionYeastAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-2X(CMV-eBFP2-3XNLS-pA)
Plasmid#195867Purposeto express nuclear localized eBFP2DepositorInserteBFP2
UseAAVPromoterCMVAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-NLS-MaSat ZF-18-mVenusN
Plasmid#191392PurposeZinc finger fused to N-terminal part of split mVenusDepositorInsertFlag-NLS-MaSat ZF-18-mVenusN
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-UbC-3xNLS-mScarlet-I
Plasmid#191099PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-mScarlet-I
UseAAV and Synthetic BiologyTagsThree repeats of the nuclear localizzation seque…ExpressionMammalianMutationoptimized to human codon usagePromoterUbCAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-3x-NLS-mScarlet-AMPKα2
Plasmid#192452PurposemScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.DepositorInsert3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
Tags3x Nuclear localization signal (NLS) and mScarletExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-ArgSen (-)NLS
Plasmid#184689PurposeLentiviral transfer plasmid for expression of ArgSen (-)NLSDepositorInsertArgSen (-)NLS
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-ArgSen (-)NLS
Plasmid#184698PurposeLentiviral transfer plasmid for expression of ArgSen (-)NLSDepositorInsertArgSen (-)NLS
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
nPPARg429-gs10-NLS-VPR
Plasmid#188266PurposePlasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnPPARg429 (PPARG Human)
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-NLSlacZ-stop-L2
Plasmid#186358PurposeEntry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.DepositorInsertlacZ (lacZ Synthetic, E. coli)
UseExpression of nuclear lacz reporterTagsNuclear localization signalAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-NLP-cMyc AspCas12a
Plasmid#182120PurposepET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteriaDepositorInsert2xNLS-NLP-cMyc AspCas12a
Tags6xHisExpressionBacterialPromoterT7Available SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH9-Tier1-PhCMV-3xNLS
Plasmid#169478PurposeTier-1 vector encoding PhCMV-driven triple nuclear localization signal expression (PhCMV-3xNLS-pA).DepositorInsertPCMV-driven nuclear localization signal
ExpressionMammalianPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH8-Tier1-PhCMV-NLS
Plasmid#169477PurposeTier-1 vector encoding PhCMV-driven nuclear localization signal expression (PhCMV-NLS-pA).DepositorInsertPCMV-driven nuclear localization signal
ExpressionMammalianPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP-VP16
Plasmid#183935PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFPDepositorInserttdPCP-GFP-VP16 (UL48 Synthetic)
TagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-NLS-YFP-CCTc
Plasmid#184327PurposeMammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.2)DepositorInsertCCTc (CACNA1C Human)
ExpressionMammalianAvailable SinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-PAG-E2-Crimson-3XNLS-pA
Plasmid#183801PurposeAAV vector to express nuclear localized E2-Crimson from PAG promoterDepositorInsertE2-Crimson-NLS
UseAAVTagsNLSAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV_Hyg_FLAG-BirA-eGFP-NLS2
Plasmid#170917PurposeExpresses FLAG-BirA-eGFP-NLS2 to be used as control in BioID or FLAG pull downDepositorInsertFLAG-BirA-eGFP-NLS2
TagsBirA-FLAGExpressionMammalianAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL
Plasmid#172200PurposeAAV sequence-specific control vector for TALE-Tet1S-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianMutationtranscription regulatory domain removedPromoterhSyn human synapsin (mammalian neuronal expressio…Available SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-bNup98-DN-NLS-mClover3
Plasmid#171495PurposeT7 promotor drives in vitro transcription of mClover3-tagged bovine Nup98 DN mRNADepositorAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD684 VPR-FRB (no NLS)
Plasmid#138851PurposeConstitutive expression of VPR-FRB (no NLS) under the CMV promoter.DepositorInsertVPR-FRB (no NLS)
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD683 VP64-FRB (no NLS)
Plasmid#138850PurposeConstitutive expression of VP64-FRB (no NLS) under the CMV promoter.DepositorInsertVP64-FRB (no NLS)
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD682 VP16-FRB (no NLS)
Plasmid#138849PurposeConstitutive expression of VP16-FRB (no NLS) under the CMV promoter.DepositorInsertVP16-FRB (no NLS)
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD681 FKBP-ZF43 (no NLS)
Plasmid#138848PurposeConstitutive expression of FKBP-ZF43 (no NLS) under the CMV promoter.DepositorInsertFKBP-ZF43 (no NLS)
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD354 VP64-FRB (NLS)
Plasmid#138846PurposeConstitutive expression of VP64-FRB under the CMV promoter.DepositorInsertVP64-FRB
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD353 VP16-FRB (NLS)
Plasmid#138845PurposeConstitutive expression of VP16-FRB under the CMV promoter.DepositorInsertVP16-FRB
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD341 FKBP-ZF43 (NLS)
Plasmid#138844PurposeConstitutive expression of FKBP-ZF43 under the CMV promoter.DepositorInsertFKBP-ZF43
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-QUASM1:GFPNLS-SV40pA
Plasmid#155116PurposeMiddle entry vector containing QUASM1 element upstream of GFP-NLS.DepositorInsertQUASM1:GFPNLS
UseGateway middle entry vectorPromoterQUASM1-E1bAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E1-dTom-nlsdTom
Plasmid#135637PurposeAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E1 regulatory elementDepositorInsertdTom-nlsdTom
UseAAVMutationNoneAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E3-dTom-nlsdTom
Plasmid#135638PurposeAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E3 regulatory elementDepositorInsertdTom-nlsdTom
UseAAVMutationNoneAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E4-dTom-nlsdTom
Plasmid#135639PurposeAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E4 regulatory elementDepositorInsertdTom-nlsdTom
UseAAVMutationNoneAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E10-dTom-nlsdTom
Plasmid#135645PurposeAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E10 regulatory elementDepositorInsertdTom-nlsdTom
UseAAVMutationNoneAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSHF1-P2A-Puro
Plasmid#136901PurposeLentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized)DepositorInsertFNLSHF1
UseLentiviralTagsFLAG-NLS and NLSMutationR661A,Q695A,Q926APromoterEF1sAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a-3NLS-Sox2TALE-GCN4
Plasmid#120548PurposeExpress 3xNLS-Sox2 TALE-GCN4 engineered to bind a site in the human SOX2 geneDepositorAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a-3NLS-NGN2TALE-GCN4
Plasmid#120547PurposeExpress 3xNLS-NGN2 TALE-GCN4 engineered to bind a site in the human NGN2 eneDepositorAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSD_FRB-TALETS3-3xFLAG-2xNLS
Plasmid#107306PurposeExpresses TALE-VEGFA-TS3 fused to FRB in mammalian cellsDepositorInsertTALE_VEGFA-TS3
UseCRISPRTags3x Flag, 2xNLS and FRBExpressionMammalianPromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-hGCN5-NLS
Plasmid#103827PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorExpressionMammalianMutationhGCN5: A566G (E189G), G585T (K195N), G726A (silen…PromoterCMVAvailable SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
DsRed-CPI-17-T38A-NLS
Plasmid#100792PurposeMammalian expression of Fluorescent CPI-17 neutral mutation nuclearDepositorInsertprotein phosphatase 1 regulatory inhibitor subunit 14A (PPP1R14A Human)
TagsDsRedExpressionMammalianMutationT38APromoterCMVAvailable SinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
DsRed-CPI-17-T38E-NLS
Plasmid#100790PurposeMammalian expression of Fluorescent CPI-17 phospho mimetic nuclearDepositorAvailable SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only