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Plasmid#92105PurposeExpression in eukaryotic cellsDepositorInsertMefv isoform1 (Mefv Mouse)
UseTags3xHAExpressionMammalianMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGG-cTP-TurboID
Plasmid#209394PurposeTransiently expressing TurboID fused with a chloroplast transit peptide (cTP) in plantaDepositorInsertcTP-3XHA-TurboID
UseTagsExpressionPlantMutationPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID
Plasmid#209393PurposeTransiently expressing TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
UseTagsExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME18S-3HA-mULK1
Plasmid#22896DepositorInserta novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
UseTags3xHAExpressionMammalianMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
BPK2013-AkCas12b
Plasmid#121950PurposeE. coli expression, protein purificationDepositorInsertAkCas12b
UseTags10xHis tag and 3xHA tagExpressionBacterialMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLBC0060
Plasmid#172783PurposeLevel 0 module containing NT2_cYFP3XHADepositorInsertCDS C-terminal eYFP (175-238) 3XHA tag
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBC0061
Plasmid#172784PurposeLevel 0 module containing CT_cYFP3XHADepositorInsertCDS C-terminal eYFP (175-238) 3XHA tag
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BsCas12b-2AeGFP
Plasmid#121948PurposeMammalian expression, Genome editingDepositorInsertBsCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AmCas12b-2AeGFP
Plasmid#121947PurposeMammalian expression, Genome editingDepositorInsertAmCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOG658
Plasmid#105409PurposeSynthetic biologyDepositorInsert3xHA tag fused to Luciferase CDS
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG642
Plasmid#105406PurposeSynthetic biologyDepositorInsert3xHA tag fused to tagRFP-T CDS
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 SATtoG KtoRHA
Plasmid#232994PurposeGalactose iduced expression of Gcn4 SATtoG KtoRHAin yeastDepositorInsertGcn4 SATtoG KtoR
UseTags3xHA and TEV cleavage siteExpressionYeastMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 solvvol W
Plasmid#232977PurposeGalactose iduced expression of Gcn4 solvvol W+ in yeastDepositorInsertGcn4 solvvol W+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
RT-4
Plasmid#220443PurposeConstruct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.DepositorInsertPUF3 (PUF3 Budding Yeast)
UseTags3xHAExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-GTS-TurboID
Plasmid#209397PurposeTransiently expressing TurboID fused with a Golgi targeting sequence (GTS) in plantaDepositorInsertGTS-3XHA-TurboID
UseTagsExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-doraWT_rescue_construct
Plasmid#190608PurposePuromycin-selectable expression of HA-tagged Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationPromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
BPK2013-BsCas12b
Plasmid#121952PurposeE. coli expression, protein purificationDepositorInsertBsCas12b
UseTags10xHis tag and 3xHA tagExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOG659
Plasmid#105410PurposeSynthetic biologyDepositorInsertLuciferase CDS fused to 3xHA tag
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
YCp33-YDL183c-HA
Plasmid#70164Purposeyeast expression of YDL183c from its own promoter and in fusion with a triple HA epitope at the C-terminal endDepositorInsertYDL183c
UseTags3xHAExpressionYeastMutationPromoterYDL183c (217 nucleotides upstream of the ATG)Available sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-TurboCas-NLS-Blast
Plasmid#232346PurposeLentiviral expression vector for dCas9 fused to miniTurboNLSDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA, miniTurbo, V5 and 3xSV40 NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only