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nCre-ST-NLS-P2A-NLS-pSC-cCre

Plasmid

#207638

Purpose

A plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequence

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

nCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre

Use

Tags

Expression

Mammalian

Mutation

Amber stop codon at SpyCatcher’s critical lysine

Promoter

CMV

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-GCGR-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194377

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-GCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-GLP1R-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194381

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA-10Xcapture-PuroR

Plasmid

#211496

Purpose

Plasmid for the expression of SAM-compatible gRNA for CRISPRa with capturer sequence for 10X Genomics sequencing

Depositor

Antonella Fidanza, Lesley Forrester

Article

Petazzi et al eLife. 2024 Dec 23;13:RP94884. doi: 10.7

Inserts

U6-gRNA
Ef1a-PuroR

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1a and U6

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-HTR2A-NTEV-TCS-GV-2xHA

Plasmid

#194367

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-HTR2A-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_HTR2A-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194368

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

HTR2A-V2R-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-HTR2A-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194369

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-HTR2A-V2R-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_AVPR2-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194372

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

AVPR2-V2R-NTEV-TCS-GV-2xHA (AVPR2 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-AVPR2-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194373

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-AVPR2-V2R-NTEV-TCS-GV-2xHA (AVPR2 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-GCGR-NTEV-TCS-GV-2xHA

Plasmid

#194375

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-GCGR-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_GCGR-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194376

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

GCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-DRD1-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194361

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-DRD1-V2R-NTEV-TCS-GV-2xHA (DRD1 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-DRD2-NTEV-TCS-GV-2xHA

Plasmid

#194363

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-DRD2-NTEV-TCS-GV-2xHA (DRD2 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_DRD2-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194364

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

DRD2-V2R-NTEV-TCS-GV-2xHA (DRD2 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_SP-DRD2-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194365

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-DRD2-V2R-NTEV-TCS-GV-2xHA (DRD2 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pET-NCBD + ACTR

Plasmid

#99335

Purpose

Bacterial coexpression of CBP NCBD and NCoA3 binding domains

Depositor

Peter Wright

Article

Demarest et al Nature. 2002 Jan 31;415(6871):549-53.

Inserts

CBP NCBD (2059-2117) (CREBBP Mouse)
SRC3 (1023-1093) (NCOA3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19_18GZG

Plasmid

#117228

Purpose

shble flanked by an endogenous GAPDH promoter and terminator system

Depositor

Jackie Collier

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Insert

shble

Use

Thraustochytrid expression

Tags

Expression

Mutation

Promoter

GAPDH

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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DinoIII-neo

Plasmid

#155026

Purpose

Dinoflagelate backbone for amino 3'-glycosyl phosphotransferase (neo) gene

Depositor

Senjie Lin

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Insert

amino 3'-glycosyl phosphotransferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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DinoIII-pat

Plasmid

#155028

Purpose

Dinoflagelate backbone for Phosphinothricin N-acetyltransferase, pat, gene

Depositor

Senjie Lin

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Insert

Phosphinothricin N-acetyltransferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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DinoIII-bsr

Plasmid

#155027

Purpose

Dinoflagelate backbone for blasticidin S-resistance gene (bsr)

Depositor

Senjie Lin

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Insert

Blasticidin-S deaminase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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