We narrowed to 11,977 results for: pcDNA

1. pcDNA flag PPAR gamma E499Q

   Plasmid

   #8896

   Depositor

   Bruce Spiegelman

   Article

   Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

   Insert

   PPAR gamma (Pparg Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   E499Q, a mutant in the AF2 domain. Binds PPARg l…

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-Lifeact-(GGS)X6-HaloTag C58

   Plasmid

   #171435

   Purpose

   Expression of Lifeact-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   Lifeact-(GGS)X6-HaloTag C58
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. (133) pcDNA3.1-Nup62-Flag-EPEA

   Plasmid

   #160744

   Purpose

   Expresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   Nup62-Flag-EPEA
   

   Use

   Tags

   EPEA and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA5/FRT/TO-[4xCox8]-ChemoG-NAD

   Plasmid

   #193823

   Purpose

   CMV overexpression of ChemoG-NAD in the mitochondria of mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   [4xCox8]-ChemoG-NAD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:A206K, T225R; HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 Plk4(Sak) wt (Nigg HR9)

   Plasmid

   #41165

   Depositor

   Erich Nigg

   Article

   Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

   Insert

   Plk4 (PLK4 Human)
   

   Use

   Tags

   3xMyc and PreScission Site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA6-IgK-hFRa(23-234)C11S-th-Fc

   Plasmid

   #177841

   Purpose

   Mammalian Expression of Folate Receptor

   Depositor

   Karsten Melcher

   Article

   Chen et al Nature. 2013 Aug 22;500(7463):486-9. doi

   Insert

   Folate receptor alpha (FOLR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   all 11 C mutated to S

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

   Plasmid

   #175529

   Purpose

   Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Cox8a-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-HA-CUL1 delta 610-615

   Plasmid

   #19941

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

   Insert

   cullin 1 delta 610-615 (CUL1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   CUL1 with a ROC1 binding mutant

   Promoter

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA-Ran-Q69L-mRFP1-polyA

   Plasmid

   #104560

   Purpose

   Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Ran (Ran Mouse)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Q69L

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-EGFP MASTL WT (siRNA resistant)

    Plasmid

    #191011

    Purpose

    Expresses EGFP-tagged MASTL WT with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

    Depositor

    Johanna Ivaska

    Article

    Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Microtubule-associated serine/threonine kinase like (MASTL Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 FLAG-SIV-MAC251-vpx

    Plasmid

    #115842

    Purpose

    Encodes codon optimized FLAG tagged SIVMAC251-Vpx

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251-Vpx
    

    Use

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5953 pcDNA-Xpress-His-USP15

    Plasmid

    #23216

    Depositor

    Peter Howley

    Article

    Vos et al J Virol. 2009 Sep . 83(17):8885-92.

    Insert

    USP15 (USP15 Human)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA 3.1 LIC 6A NLRP1-FLAG

    Plasmid

    #166803

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3.1-Flag-H2A K118-119R

    Plasmid

    #63564

    Purpose

    Expression of human H2A with K118R and K119R tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine 118 and Lysine 119 are now Arginine

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3 Flag TSC2 (S939A/T1462A)

    Plasmid

    #14131

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 S939A/T1462A (TSC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S939A/T1462A

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-iPASnFR-L-mScarlet3-3xFLAG-Rab5

    Plasmid

    #225244

    Purpose

    Mammalian expression of iPASnFR-L fused to Rab5

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-L
    

    Use

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon

    Plasmid

    #123353

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-Cameleon
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4c/His-Xpress-HA-hBIG1(E793K)

    Plasmid

    #79433

    Purpose

    Expresses N-terminally His6, Xpress and HA-tagged BIG1(E793K) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

    Insert

    BIG1 (ARFGEF1 Human)
    

    Use

    Tags

    HA, His6, and Xpress

    Expression

    Mammalian

    Mutation

    changed Glutamic acid 793 to Lysine

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA-miR-155 toehold switch

    Plasmid

    #184755

    Purpose

    Expresses miR-155 sensor with GFP output

    Depositor

    Allen Liu

    Article

    Wang et al ACS Synth Biol. 2019 May 17;8(5):1079-10

    Insert

    Green Fluorescence Protein
    

    Use

    Tags

    miR-155 toehold

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-mCherry-mCherry-FLARE-AKAR

    Plasmid

    #123333

    Purpose

    Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    mCherry-mCherry-FLARE-AKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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