We narrowed to 13,694 results for: NTS

1. pS6-TGFB2-H8

   Plasmid

   #128502

   Purpose

   Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t H8x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   TGFB2 (L21-S414) (TGFB2 Human)
   

   Use

   Tags

   His tag (6x)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-Puro_mouse_Adar2_Tv1

   Plasmid

   #158111

   Purpose

   expresses mADAR2 shortest transcript, puromycin selection

   Depositor

   Emanuela Felley-Bosco

   Article

   Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

   Insert

   adar2 (Adarb1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_SLC2A3_WT

   Plasmid

   #81787

   Purpose

   Gateway Donor vector containing SLC2A3 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SLC2A3 (SLC2A3 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCerulean3-Bcl-W-FUGW

   Plasmid

   #166751

   Purpose

   Express mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   Bcl-W (Bcl2l2 Mouse)
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mutation

   Promoter

   human ubiquitin C

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y

   Plasmid

   #164845

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tag

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
   

   Use

   Tags

   8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression. Has N5…

   Promoter

   CMV

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pROF346

   Plasmid

   #155331

   Purpose

   Binary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   RB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pD2_miniluc_newP7

   Plasmid

   #174105

   Purpose

   adding the mini luciferase and mini promoter to the MPRA lenti construct

   Depositor

   Paul Khavari

   Article

   Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

   Insert

   mini luciferase mini-promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_PPP6C_p.R264C

   Plasmid

   #81341

   Purpose

   Gateway Donor vector containing PPP6C , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PPP6C (PPP6C Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   R264C

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. BM00369

   Plasmid

   #155338

   Purpose

   Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling GUS. It constitutively expresses dsRed and a plant selection cassette.

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-dsRed-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-GUS-POpto_LB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)

    Plasmid

    #160623

    Purpose

    TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35S:dCas9:VPR-Tnos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_PRKCZ_WT

    Plasmid

    #82228

    Purpose

    Gateway Donor vector containing PRKCZ , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PRKCZ (PRKCZ Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_SMURF2_WT

    Plasmid

    #82259

    Purpose

    Gateway Donor vector containing SMURF2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SMURF2 (SMURF2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. AttB_[Kozak-mut]ACE2_IRES-mCherry-H2A-P2A-PuroR

    Plasmid

    #171595

    Purpose

    Weak human ACE2 abundance from Bxb1 landing pad

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    ACE2 (ACE2 Human)
    mCherry
    H2A
    Pac
    

    Use

    Synthetic Biology; Promoterless bxb1 dna recombin…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.CAG-FLEX.SF-iGluSnFR.S72A.mRuby3

    Plasmid

    #106206

    Purpose

    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.S72A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: S72A + mRuby3 fusion

    Promoter

    CAG

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cry2 mCherry GRK2 ct in pcDNA3.1

    Plasmid

    #64212

    Purpose

    mCherry fused between Cry2 and GRK2ct to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    GRK2 ct (GRK2 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and cmv

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pDONR223_MAGEC2_WT

    Plasmid

    #81862

    Purpose

    Gateway Donor vector containing MAGEC2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    MAGEC2 (MAGEC2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRE

    Plasmid

    #139088

    Purpose

    Expresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    xCas9 3.7
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    A262T, R324L,S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJT311 AAV-Rapamycin Inducible-NZF-Citrine

    Plasmid

    #202060

    Purpose

    Inducible AAV activator

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    AAV-Rapamycin Inducible-NZF-Citrine
    

    Use

    AAV; Aav with reporter

    Tags

    Expression

    Mammalian

    Mutation

    ITR deletion

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

    Plasmid

    #114087

    Purpose

    U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for FnCas12a crRNAs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV.CAG.SF-Venus-iGluSnFR.A184V

    Plasmid

    #106202

    Purpose

    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.A184V
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

    Promoter

    CAG

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGM234

    Plasmid

    #220177

    Purpose

    Expresses exogenous wild-type NAC complex members (NACA and BTF3)

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    NACA and BTF3 (NACA, BTF3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG (NACA); 6xHis (BTF3)

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pVD1 M1_1-pTRNA-scf 2.1 (GB2603)

    Plasmid

    #160568

    Purpose

    tRNA and scaffold 2.1 scRNA aptamer Ms2 for the assembly of GBoligomers for a single position of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    M1_1-pTRNA-scf 2.1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

    Plasmid

    #114089

    Purpose

    CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized FnCas12a
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Cry2 mCherry GRK3 ct in pcDNA3.1

    Plasmid

    #64213

    Purpose

    mCherry fused between Cry2 and GRK3 ct to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    GRK3 ct (GRK3 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pDONR223_RNF212_WT

    Plasmid

    #81860

    Purpose

    Gateway Donor vector containing RNF212 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RNF212 (RNF212 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    W4L

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTE4889

    Plasmid

    #88905

    Purpose

    Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Fn crRNA
    hFnCpf1(D917A)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR223_PPP6C_p.E180K

    Plasmid

    #81334

    Purpose

    Gateway Donor vector containing PPP6C , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PPP6C (PPP6C Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    E180K

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139091

    Purpose

    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dSpCas9-NG-VPR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 3322_pETcon_SARS2_Omicron-BA2

    Plasmid

    #184409

    Purpose

    yeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    G339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 416Gal-SV40NLS-FUS-YFP

    Plasmid

    #29595

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    NLS-FUS (FUS Human)
    

    Use

    Tags

    SV40 NLS and YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only