We narrowed to 24,925 results for: crispr
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Plasmid#132408Purposeoverexpression in human cellsDepositorInsertdLbaCas13b
UseLentiviralTagsflagExpressionMammalianMutationR600A; H605A; R1243A; H1248AAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#112922PurposeGateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
UseGateway CloningExpressionMammalianAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP
Plasmid#114729Purpose3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA M5, sgRNA M7, sgRNA M9
UseCRISPRExpressionMammalianAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOD8A-iRGR-r11
Plasmid#74369PurposeThis plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorPublicationInsertRGR-r11
UseCRISPR and Synthetic BiologyExpressionYeastPromoterAHD1Available sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_IL1RN
Plasmid#64140PurposePhotoactivatable transcription system. Lentiviral expression of IL1RN sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA4_MYOD1
Plasmid#64139PurposePhotoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_MYOD1
Plasmid#64136PurposePhotoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.DepositorAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA2_MYOD1
Plasmid#64137PurposePhotoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette.DepositorAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX52
Plasmid#169444PurposeUsed for guide cloning with PCRDepositorTypeEmpty backboneUseCRISPRAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC1813_pCR8-gSMN2-DN1
Plasmid#118646PurposeTransient transfection; Expressed SMN2-DN1 CasRx gRNA; Gateway DonorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgSMN2-DN1
UseCRISPRExpressionMammalianPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGMC00018 (aka pRC0082)
Plasmid#195320PurposeMinimal plasmid backbone with multiple cloning site for HDR donor DNA constructionDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterial and MammalianAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKSB-618 U6-sgRNA2/3 GGAA AACA
Plasmid#173674PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYDE009
Plasmid#194151PurposeDelivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.DepositorInserttetR-tetP-dcas9-rrnB T1-T7Te
ExpressionBacterialPromotertetpAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPACE-ΔUGI (pRZ1836)
Plasmid#140243PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSPACE-ΔUGI
ExpressionMammalianMutationV82G in TadA*, D10A in Cas9, R187W in pmCDA1PromoterCMVAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA
Plasmid#82705PurposeAAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.DepositorInsertU6/TO gRNA Expression Cassette Containing Tet2 gRNA
UseAAV and CRISPRTagsCMV promoter driving expression of TetR-P2A-GFP-K…ExpressionMammalianAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJKW0458
Plasmid#107587Purposep35S:CAS9DepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterp35SAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
VPR_dCas9
Plasmid#134601PurposeExpress CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVPR-dCas9-2A-ZsGreen-DR
TagsVPR and ZsGreen1-DRExpressionMammalianPromoterCMVAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
STAGR_gRNAScaffold_mU6
Plasmid#102844PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert gRNAScaffold_mU6
UsePcr template for stagr insertsPromotermU6Available sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSDM052
Plasmid#194341PurposepUC mini-Tn7 vector expressing LseCas13aDepositorInsertCas13a(Lse)
ExpressionBacterialAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNuc-cis
Plasmid#202796PurposeRK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killingDepositorInsertTevSpCas9, sgRNA cassette, oriT, CenArsHis
ExpressionBacterialAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only