Addgene

We narrowed to 21,422 results for: ESC;

Showing: 8821 - 8850 of 21422 results

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mOrange-H2B-6

Plasmid

#57942

Purpose

Localization: Nucleus/Histones, Excitation: 548, Emission: 562

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Insert

H2B (H2BC11 Human)

Use

Tags

mOrange

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-NES-MBP-cATOM(Ras)

Plasmid

#209711

Purpose

Mammalian expression of c-ATOM biosensor for HRas with nuclear export signal

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

NES-MBP-cATOM(Ras)

Use

Tags

NES-MBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mIFP-P2A-GFP

Plasmid

#197168

Purpose

Expresses the protein of mIFP-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mIFP-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pOVC1_EcSSPB-nano_X

Plasmid

#131212

Purpose

Expresses EcSSPB-nano (Escherichia coli) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

EcSSPB-nano

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-HCA_sensor

Plasmid

#127182

Purpose

Eukaryotic expression of the fusion protein used for sulfa-drugs sensing

Depositor

Kai Johnsson

Article

Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

Insert

SNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA

Use

Tags

HisTag and StrepTag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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Dendra2-Gamma-Tubulin-17

Plasmid

#57721

Purpose

Localization: Centrosomes, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Gamma-Tubulin (TUBG1 Human)

Use

Tags

Dendra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19/mChe-Cs

Plasmid

#183171

Purpose

Construct for transient expression of cytoskeleton-targeted mCherry in plants.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

mCherry-Cs

Use

Tags

363 aa of A. thaliana FIMBRIN 1 (AtFIM1)

Expression

Plant

Mutation

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRU51

Plasmid

#167677

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::spCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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attB-GFP

Plasmid

#65521

Purpose

Plasmid for the generation of GFP-knockin fusions via Bxb1-mediated recombination into MIN-tagged cell lines

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA

Plasmid

#108236

Purpose

Broad host bacterial expression vector with constitutive PsbA promoter

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

PsbA promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-agrBD-I

Plasmid

#53439

Purpose

T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrBD locus

Use

Synthetic Biology

Tags

V5 epitope, 6x HIS (not expressed on agrB or agrD…

Expression

Bacterial

Mutation

none

Promoter

T7-lac

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST-hPDI

Plasmid

#60555

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Article

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Use

Tags

His6-hPDI

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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mEmerald-CENPB-N-22

Plasmid

#54037

Purpose

Localization: Nucleus/Centromeres, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

CENPB (CENPB Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
SBP-mCherry-Ecadherin_puromycin

Plasmid

#65293

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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mOrange2-H2B-6

Plasmid

#57962

Purpose

Localization: Nucleus/Histones, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Insert

H2B (H2BC11 Human)

Use

Tags

mOrange2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pETM6-At4CL-CmCHS-MsCHI

Plasmid

#73394

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
CmCHS
MsCHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046959

Plasmid

#145722

Purpose

Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046763

Plasmid

#145606

Purpose

Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;FLAG_2

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFOX6-Tier1-PhCMV-TdTomato

Plasmid

#169515

Purpose

Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven dimeric red fluorescent protein TdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR.R205A

Plasmid

#112178

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.R205A

Use

Tags

Expression

Mammalian

Mutation

R205A (no GABA binding)

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDGE8

Plasmid

#79463

Purpose

sgRNA shuttle vector; module 2E

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046879

Plasmid

#149238

Purpose

Bacterial expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

May 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCytERM-mTagBFP2

Plasmid

#184940

Purpose

ER Visualization (OSER assay) Expresses mTagBFP2 in fusion with N-terminal part of p450

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

CytERM-mTagBFP2

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-hPDIb′a′

Plasmid

#60556

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Article

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Use

Tags

His6-hPDIb'a' tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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mStrawberry-Vimentin-7

Plasmid

#55945

Purpose

Localization: Intermediate Filaments, Excitation: 574, Emission: 596

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Vimentin (VIM Human)

Use

Tags

mStrawberry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.iGABASnFR.F102G

Plasmid

#112167

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Tags

Expression

Mutation

F102G

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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ManII-SBP-EGFP_puromycin

Plasmid

#65260

Purpose

ManII reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated Mannosidae II

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac1 PIK3R1-Ni

Plasmid

#17744

Depositor

Bert Vogelstein

Article

Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

Insert

PIK3R1-Ni (PIK3R1 Human)

Use

Tags

Expression

Insect

Mutation

Partial cDNA

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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mRuby2-MannII-N-10

Plasmid

#55903

Purpose

Localization: Mannosidase/Golgi, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

MannII

Use

Tags

mRuby2

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1: TfR1-CoilE

Plasmid

#181988

Purpose

CoilE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

TfR1-CoilE

Use

Tags

CoilE

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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