We narrowed to 39,223 results for: ANT

1. Venus1-WTSTAT3

   Plasmid

   #123164

   Purpose

   Expresses wild type STAT3 fused to the 1-157 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
   

   Use

   Tags

   Venus1 (aa 1-158) for bimolecular fluorescence co…

   Expression

   Mammalian

   Mutation

   none

   Promoter

   pCMV

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRE-TDP-43ΔNLS-mClover3

   Plasmid

   #214916

   Purpose

   inducible expression of TDP-43 harboring mutant NLS and C-terminal mClover3. Expression yields cytosolic diffuse TDP-43

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   TDP-43ΔNLS (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   mClover3

   Expression

   Mutation

   mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

   Promoter

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-MKK1K97A

   Plasmid

   #107974

   Purpose

   expression of a dominant negative MAP Kinase Kinase

   Depositor

   Edwin Krebs

   Article

   Seger et al J Biol Chem. 1994 Oct 14;269(41):25699-7

   Insert

   MKK1 K97A (MAP2K1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dominant negative; see Depositor Comments below

   Promoter

   CMV

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLED.NPL

   Plasmid

   #193014

   Purpose

   Neuron-specific reporter (CBh promoter, Pls3 exon, bichromatic reporter)

   Depositor

   Jonathan Ling

   Article

   Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

   Insert

   Bichromatic reporter (EGFP and dsRed-Express2)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIGF1_P1 Promoter-FLuc

   Plasmid

   #154266

   Purpose

   Firefly luciferase reporter containing the canonical Human IGF-1 P1 Promoter.

   Depositor

   Antonio Amelio

   Article

   Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

   Insert

   IGF-1 canonical P1 Promoter (IGF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human IGF-1 P1 Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS/XBB.1.16-RBD

   Plasmid

   #213070

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.16 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pCAGGS/XBB.1.16-RBD (S )
   

   Use

   Tags

   6 His Tag

   Expression

   Mammalian

   Mutation

   G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLX311-GFP-MEKDD

   Plasmid

   #194882

   Purpose

   Expression of dominant negative MEK1

   Depositor

   Sefi Rosenbluh

   Article

   Tuano et al Genome Biol. 2023 Mar 29;24(1):59. doi:

   Insert

   MEKDD (MAP2K1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   S218D, S222D

   Promoter

   E1Fa

   Available since

   March 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV x EF1a_EKAREN4-ires-blast

   Plasmid

   #167829

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Venus2-WTSTAT3

   Plasmid

   #123165

   Purpose

   Expresses wild type STAT3 fused to the 158-238 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
   

   Use

   Tags

   Venus2 (aa 158-238) for bimolecular fluorescence …

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBK2045-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(CMV-gRNA1)

    Plasmid

    #223165

    Purpose

    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting CMV

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. miniTol2 x EF1a_EKAREN5 + PGK-puro

    Plasmid

    #167817

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-YFP-KRAS4B

    Plasmid

    #112718

    Purpose

    Expresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.

    Depositor

    Kenneth Westover

    Article

    Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

    Insert

    YFP-KRAS4B (KRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMVenh synapsin-intron-aSYNUCLEIN A53T

    Plasmid

    #227004

    Purpose

    AAV transfer plasmid encoding the human A53T mutant α-SYN under the control of the CMVie enhanced synapsin1 promoter

    Depositor

    Veerle Baekelandt

    Article

    Van der Perren et al J Vis Exp. 2016 Feb 28;(108):53670. doi:

    Insert

    alpha synuclein A53T mutant (SNCA Human, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A53T

    Promoter

    CMVenh synapsin

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti CMV TRE3G Neo GFP-Progerin C661S

    Plasmid

    #118711

    Purpose

    Lentiviral TET-ON inducible GFP-Progerin C661S (Farnesylation mutant)

    Depositor

    Tom Misteli

    Article

    Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

    Insert

    GFP-Progerin C661S (LMNA Human)
    

    Use

    Lentiviral; Tet-on inducible

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C661S farnesylation mutant

    Promoter

    CMV TRE3G (TET-ON)

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL1 sgRNA(MS2)-MS2-AIDmono

    Plasmid

    #112127

    Purpose

    empty sgRNA cloning vector with MS2-AIDmono

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    AID mono mutant (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    N- mutated and C- terminal truncated, H130A/R131E…

    Promoter

    hU6,CBh

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDmelOR

    Plasmid

    #126476

    Purpose

    Vector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intron

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    DmelOrco (Orco Fly)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens; includes a rec…

    Promoter

    CMV

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LV-AR-KKKAAA

    Plasmid

    #171219

    Purpose

    Lenti-viral expression of human androgen receptor DNA-binding mutant: K630A-K632A-K633A, mutations at the boundary between AR DNA-binding domain and hinge domain.

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR-KKKAAA (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-K630A-K632A-K633A

    Promoter

    EF-1a

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVD1 Multiplexing Dicot Edit E1_NTERM (GB2245)

    Plasmid

    #160567

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for position [D1_n] of a monocistronic tRNA-gRNA

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    Multiplexing Dicot Edit E1_NTERM (Multiplexing Dicot Edit)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMX-HA-UbvG08_MUT-IRES-GFP

    Plasmid

    #75031

    Purpose

    Retroviral vector with ubiquitin variant with reverted mutations L69P and V70L (i53-DM mutant)

    Depositor

    Daniel Durocher

    Article

    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

    Insert

    Ubiquitin
    

    Use

    Retroviral

    Tags

    HA and IRES-eGFP

    Expression

    Mutation

    UbvG08 with I44A, P69L, and L70V mutations and no…

    Promoter

    Available since

    June 30, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pSLED.GluA2

    Plasmid

    #193017

    Purpose

    GluA2 (Gria2) flip/flop reporter (hSyn promoter, Gria2 flip/flop exons, bichromatic reporter)

    Depositor

    Jonathan Ling

    Article

    Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

    Insert

    Bichromatic reporter (EGFP and dsRed-Express2)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Venus1-S727A-STAT3

    Plasmid

    #123174

    Purpose

    Expresses S727A STAT3 fused to the 1-157 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    

    Use

    Tags

    Venus1 (aa 1-158) for bimolecular fluorescence co…

    Expression

    Mammalian

    Mutation

    S727A substitution

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSLED.ENS

    Plasmid

    #193016

    Purpose

    Excitatory neuron-specific reporter (hSyn promoter, Synrg exon, bichromatic reporter)

    Depositor

    Jonathan Ling

    Article

    Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

    Insert

    Bichromatic reporter (EGFP and dsRed-Express2)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLL3 sgRNA(MS2)-MS2-AID3f

    Plasmid

    #112129

    Purpose

    empty sgRNA cloning vector with MS2-AID3f

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    AIDmono 3f mutant (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    N- mutated and C- terminal truncated, F115Y/C116F…

    Promoter

    hU6,CBh

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLL2 sgRNA(MS2)-MS2-AID3c

    Plasmid

    #112128

    Purpose

    empty sgRNA cloning vector with MS2-AID3c

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    AID mono 3c mutant (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    N- mutated and C- terminal truncated, F115Y/C116F…

    Promoter

    hU6,CBh

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBK2047-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

    Plasmid

    #223167

    Purpose

    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Venus1-Y705F-STAT3

    Plasmid

    #123172

    Purpose

    Expresses Y705F STAT3 fused to the 1-157 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    

    Use

    Tags

    Venus1 (aa 1-158) for bimolecular fluorescence co…

    Expression

    Mammalian

    Mutation

    Y705F substitution

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAGGS/XBB.1.5-RBD

    Plasmid

    #213038

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pCAGGS/XBB.1.5-RBD (S )
    

    Use

    Tags

    6 His Tag

    Expression

    Mammalian

    Mutation

    G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. miniTol2 x EF1a_EKAREN5 + PGK-blast

    Plasmid

    #167818

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-CFP-KRAS4B

    Plasmid

    #112717

    Purpose

    Expresses CFP-KRAS4B fusion protein used for FRET studies in mammalian cells.

    Depositor

    Kenneth Westover

    Article

    Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

    Insert

    CFP-KRAS4B (KRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-Flag::UbvG08 P69L, L70V, I44A, deltaGG

    Plasmid

    #74940

    Purpose

    Ubiquitin variant with mutations L69P and V70L reverted to wild type (i53-DM mutant)

    Depositor

    Daniel Durocher

    Article

    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

    Insert

    Ubiquitin
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    UbvG08 with I44A, P69L, and L70V mutations and no…

    Promoter

    CMV

    Available since

    May 13, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge