We narrowed to 146,921 results for: ins;

1. pET28 sf:em-blue-shift.1

   Plasmid

   #191928

   Purpose

   Encodes a GFP design, sf:em-blue-shift.1

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   sf:em-blue-shift.1
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   T65S Q69P S72A T108V Y145M T167V V224I

   Promoter

   T7

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgVRK2-puro

   Plasmid

   #199636

   Purpose

   sgRNA guide against VRK2

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A (VRK2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPIP1;4-NLS-3xmCitrine

   Plasmid

   #192524

   Purpose

   Fluorescent reporter for plant responses to bacterial infection

   Depositor

   Gitta Coaker

   Article

   Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

   Insert

   PLASMA MEMBRANE INTRINSIC PROTEIN 1;4 (PIP1;4 Mustard Weed)
   

   Use

   Tags

   3xmCitrine and NLS

   Expression

   Plant

   Mutation

   Promoter

   PIP1;4

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX317-VRK2-K168E

   Plasmid

   #199642

   Purpose

   Expresses kinase-inactive VRK2 (K168E)

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   VRK2 (VRK2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   K168E

   Promoter

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ptAD-seq-ubi63E-Gal4-DBD

   Plasmid

   #111930

   Purpose

   Vector to express tAD candidates from a library cloned from fragmented transcription factor coding sequences fused to the Gal4 DNA binding domain.

   Depositor

   Alexander Stark

   Article

   Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

   Insert

   tAD-seq Gal4-DBD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc5.1B-EGFP-DmNanos_AC

   Plasmid

   #148455

   Purpose

   Insect Expression of DmNanos

   Depositor

   Elisa Izaurralde

   Article

   Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

   Insert

   DmNanos (nos Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   sequence contains a deletion of N283 and a mutati…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAc5.1B-EGFP-DmNanos_1-296_AC

   Plasmid

   #148456

   Purpose

   Insect Expression of DmNanos_1-296

   Depositor

   Elisa Izaurralde

   Article

   Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

   Insert

   DmNanos_1-296 (nos Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   sequence contains a deletion of N283 compared to …

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-lambdaN-HA-DmNanos_AC

   Plasmid

   #148460

   Purpose

   Insect Expression of DmNanos

   Depositor

   Elisa Izaurralde

   Article

   Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

   Insert

   DmNanos (nos Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   sequence contains a deletion of N283 compared to …

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1B-lambdaN-HA-DmNanos_1-296_AC

   Plasmid

   #148461

   Purpose

   Insect Expression of DmNanos_1-296

   Depositor

   Elisa Izaurralde

   Article

   Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

   Insert

   DmNanos_1-296 (nos Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   sequence contains a deletion of N283 compared to …

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-lambdaN-HA-DmNanos_del 50-236_AC

    Plasmid

    #148462

    Purpose

    Insect Expression of DmNanos-del50-236

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    DmNanos-del50-236 (nos Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    sequence contains a deletion of N283 compared to …

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pENmRFPCNAL2 pc1054

    Plasmid

    #167567

    Purpose

    The fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.

    Depositor

    Cristina Cardoso

    Article

    Sporbert et al Nucleic Acids Res. 2005 Jun 21;33(11):35

    Insert

    Proliferating Cell Nuclear Antigen (PCNA Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28 sf:em-blue-shift.2

    Plasmid

    #191919

    Purpose

    Encodes a GFP design, sf:em-blue-shift.2

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:em-blue-shift.2
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S S72A T108I Y145F V150I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWPT-mEGFP

    Plasmid

    #190606

    Purpose

    Obtained by creating a deletion inside the mCherry coding sequence in pWPT-/GCCACC-mEGFP-IRES-mCherry (Addgene #49235)

    Depositor

    Alessandro Quattrone

    Article

    Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    a deletion was created in mCherry CDS impairing i…

    Promoter

    EIF1-short

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pACE-GIRK2-mC-S

    Plasmid

    #172428

    Purpose

    Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK2 (KCNJ6 Human)
    

    Use

    Tags

    mCherry Streptag II

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin

    Plasmid

    #182305

    Purpose

    E. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.

    Depositor

    Andrew York

    Article

    Seidel et al (2021), doi: 10.5281/zenodo.5810930

    Insert

    pH-Countdown insertion into GECO 1.2 calcium sensing domains
    

    Use

    Tags

    A modified TorA periplasmic targeting sequence pl…

    Expression

    Bacterial

    Mutation

    Spontaneous mutation of calmodulin's penulti…

    Promoter

    t7

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-lambdaN-HA-HsAGO2_E

    Plasmid

    #146224

    Purpose

    Insect Expression of HsAGO2

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

    Insert

    HsAGO2 (AGO2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Contains a K660E mutation compared to the sequenc…

    Promoter

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-lambdaN-HA-HsAGO2-F2V2_F

    Plasmid

    #146286

    Purpose

    Insect Expression of HsAGO2-F2V2

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

    Insert

    HsAGO2-F2V2 (AGO2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Contains F470V, F505V, K660E mutations compared t…

    Promoter

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pExpreS2-1-CR-PA

    Plasmid

    #175445

    Purpose

    HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    BiP Secretion Peptide and Thrombin-Protein A

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. 1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]

    Plasmid

    #149427

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Inserts

    U6.3-gRNAs[TraB, bTub]
    Hsp70Bb-Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pB80-SSPB(micro)-mVenus-ppKin14VIb(861-1321)

    Plasmid

    #174641

    Purpose

    Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    SSPB(micro)-mVenus-ppKin14VIb(861-1321)
    

    Use

    Tags

    SSPB(micro)-Venus

    Expression

    Mammalian

    Mutation

    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

    Promoter

    Chicken beta-actin

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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