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Showing: 5941 - 5960 of 6203 results
  1. pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid
    #114103
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogenetics
    Insert
    sbGLuc-VChR1-EYFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    motoneuron-specific Homeobox 9 promoter
    Available Since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)

    Plasmid
    #140893
    Purpose
    Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cel
    Depositor
    Insert
    CiDr-VSP L223F/C302S-mCherry (vsp, tpte Ciona intestinalis, Zebrafish)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    changed 223L to F in Dr-VSP (zebrafish TPTE), cha…
    Available Since
    May 13, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174644
    Purpose
    Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes
    Depositor
    Insert
    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
    Tags
    Venus-SSPB and iLID-mCherry
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…
    Promoter
    Chicken beta-actin
    Available Since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. TFORF0930

    Plasmid
    #143242
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Insert
    ZNF83 (ZNF83 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 21, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82704
    Purpose
    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Insert
    H1/TO-L Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
  6. PB-CAG-ChR2-YFP

    Plasmid
    #204345
    Purpose
    PiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.
    Depositor
    Insert
    ChR2-YFP
    Tags
    Yellow fluorescent protein
    Expression
    Mammalian
    Mutation
    C-terminus (aa315-737) is deleted - PMID: 1461559…
    Promoter
    CAG
    Available Since
    Oct. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82702
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Insert
    H1/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 5941 - 5960 of 6203 results