We narrowed to 14,742 results for: RING

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pPN-mKate (PT7LacO-PN-mKate)

Plasmid

#191126

Purpose

Expression of protein N tagged with mKate from a T7-lac inducible promoter

Depositor

Ariel Lindner

Article

Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

Insert

Protein N

Use

Synthetic Biology

Tags

mKate

Promoter

T7-lac inducible promoter

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pTALdNC-AAVS1_T1

Plasmid

#80495

Purpose

AAVS1 TALEN expression vector (Left)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

AAVS1 TALEN (Left)

Use

Gateway entry

Promoter

none

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pTALdNC-AAVS1_T2

Plasmid

#80496

Purpose

AAVS1 TALEN expression vector (Right)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

AAVS1 TALEN (Right)

Use

Gateway entry

Promoter

none

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

Plasmid

#61762

Purpose

Dual Color betaGlobin reporter to study splicing kinetics in vivo

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

b-Globin

Tags

24 X MS2 hairpin cassette in 3'UTR of b-Glob…

Expression

Mammalian

Promoter

Dox inducible Tet-ON promoter

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EGFP_Donor

Plasmid

#178088

Purpose

HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pXR003: CasRx gRNA cloning backbone

Plasmid

#109053

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.flex.H2B.GFP

Plasmid

#170368

Purpose

Cre-inducible AAV vector expressing nuclear GFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

flex H2B GFP

Use

AAV

Promoter

human Synapsin

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCS4333 yeTadA1.0-T7 RNAP

Plasmid

#137735

Purpose

pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

Depositor

Christina Smolke

Article

Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

Insert

yeTadA1.0-T7 RNAP

Expression

Bacterial and Yeast

Mutation

E366G (See depositor comment below)

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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SlugABEmax

Plasmid

#163798

Purpose

Expresses ABEmax and nSlugCas9

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Mutation

SlugCas9 (D10A)

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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SlugBE4max

Plasmid

#163799

Purpose

Expresses AncBE4max and nSlugCas9

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Mutation

SlugCas9 (D10A)

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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p4xmts-mScarlet3_N1

Plasmid

#189774

Purpose

Construct for labelling mitochondria in mammalian cells using mitochondrial targeted mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

4xmts-mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003 S49C

Plasmid

#133448

Purpose

Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003 S49C

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Contains S49C mutation

Promoter

T7

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMC-6-ZmBbm-P2A-ZmWus2-9

Plasmid

#197750

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

ZmBbm-P2A-ZmWus2 bicistronic CDS

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_LaG16_2_synNotch_Gal4VP64

Plasmid

#85421

Purpose

Lentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

LaG16_2 GFP nanobody synNotch Gal4VP64

Use

Lentiviral

Promoter

PGK

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pmGFP-P2A-K(AAA)20-P2A-RFP

Plasmid

#105688

Purpose

Double fluorescent stalling reporter K(AAA)20

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAA)20
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pX330S-2

Plasmid

#58778

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9 KRAB-T2A-GFP

Plasmid

#67620

Purpose

Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 KRAB T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(mito).cpSFGFP.HaloTag

Plasmid

#214925

Purpose

Expresses mitochondrially targeted non-responsive control

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(mito).cpSFGFP.HaloTag

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-HotPETase

Plasmid

#218701

Purpose

Encodes codon optimized HotPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

HotPETase

Tags

10xHis

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHiUGE GS-gRNA (no Cre)

Plasmid

#200370

Purpose

Backbone plasmid for cloning HiUGE GS-gRNA (without Cre)

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Type

Empty backbone

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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