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EBFP2-UtrCH

Plasmid

#124656

Purpose

F-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).

Depositor

Cheng-han Yu

Article

Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

Insert

Utrophin (UTRN Human)

Tags

EBFP2

Expression

Mammalian

Mutation

see depositor comments below

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMOS001: CMV-Optopatch2_FCK

Plasmid

#62984

Purpose

Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology under CMV promoter

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_CRFmut

Plasmid

#208656

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/TO/V5-DEST-IGFBP2-Clover

Plasmid

#191008

Purpose

Lentiviral expression vector for IGFBP2 with a fluorescent Clover tag

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)

Use

Lentiviral

Tags

Clover

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-myr-AKT-HA

Plasmid

#31790

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Use

Entry vector

Tags

HA and Myr

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only
(564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9

Plasmid

#163025

Purpose

Expression of CRISPRi with gRNA against mouse Pcsk9

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9 (Pcsk9 S. aureus)

Use

AAV

Tags

KRAB, SV40 pA, and myc NLS

Expression

Mammalian

Promoter

Alb-AAT

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-NS1-dTomato

Plasmid

#175278

Purpose

AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

NS1; dTomato (POLY Yellow fever virus strain 17D)

Use

AAV

Tags

dTomato (from bidirectional promoter)

Promoter

bidirectional TRE promoter

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 P1-6A

Plasmid

#51251

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848,923,956 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 789,805,817,848,923,956 mutated to Ala

Promoter

EF1a

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pCS2plus-mCerulean 156-239-GGGS

Plasmid

#162616

Purpose

Allows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mCerulean

Expression

Mammalian

Mutation

156-239

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep63

Plasmid

#136811

Purpose

Mammalian expression of the centrosomal protein Cep63 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep63 (CEP63 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL11060

Plasmid

#68264

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Plasmid

#78607

Purpose

A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Promoter

EF1a-short; U6

Available Since

Sept. 16, 2017

Availability

Academic Institutions and Nonprofits only
pEBTetD-SNAP-Cep27

Plasmid

#136809

Purpose

Mammalian expression of the centrosomal protein Cep27 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep27 (HAUS2 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRecLV105

Plasmid

#107238

Purpose

A lentiviral vector for expression in mammalian cells

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIH-Myc-YAP-del C

Plasmid

#33095

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

deleted C terminal (amino acids 291-450)

Promoter

CMV/MSV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pBABE MDER

Plasmid

#13494

Depositor

Stephen Tapscott

Article

Hollenberg et al Proc Natl Acad Sci U S A. 1993 Sep 1. 90

Insert

MyoD1-Estrogen Receptor Fusion Protein (Myod1 Human, Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

full length MyoD was fused to aa282-595 of Esr1. …

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS

Plasmid

#162610

Purpose

Allows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mVenus

Expression

Mammalian

Mutation

Contains aa1-155 of mVenus

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-T2A-mApple

Plasmid

#193780

Purpose

Tricistronic expression of Cas9-mApple-BSD in mammalian cells

Depositor

James Eshleman

Article

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

mApple

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-Cep63

Plasmid

#136812

Purpose

Mammalian expression of the centrosomal protein Cep63 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep63 (CEP63 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 mitoAURKA

Plasmid

#157750

Purpose

Expression of AURKA targeted to mitochondria

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Expression

Mammalian

Mutation

Aurora A without the first 30AA + MTS of CoxVIII

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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