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169,843 results

Showing: 8941 - 8970 of 169843 results

  6. pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

    Plasmid
    #104495
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV PHP.eB, AAV Retrograde, and AAV1
    Insert
    jGCaMP7s
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    104495-plasmid-map-sequence-id-200303

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  15. pMSCV-IRES-mAmetrine 1.1 FP

    Plasmid
    #52113
    Purpose
    a bicistronic IRES-mAtetrine containing retroviral vector
    Depositor
    Dario Vignali
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES-mAmetrine 1.1
    Expression
    Mammalian
    Available Since
    March 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    52113-plasmid-map-sequence-id-80499

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  17. pQCXIP-cathepsinD-YMH

    Plasmid
    #182644
    Purpose
    Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    cathepsinD
    Use
    Retroviral
    Tags
    His6-tag, TS site from proCCK, and myc-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182644-plasmid-map-sequence-id-358628

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  18. p11-LacY-wtx1

    Plasmid
    #69056
    Purpose
    The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.
    Depositor
    Huimin Zhao
    Article
    Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15
    Inserts
    ccdB (ccdB )
    lacY (lacY )
    Tags
    A177C
    Promoter
    pBAD and pLac
    Available Since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69056-plasmid-map-sequence-id-372941

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  19. pMXs-IP- SCAT3.2

    Plasmid
    #172497
    Purpose
    SCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.
    Depositor
    David Scadden
    Article
    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:
    Insert
    Amcyan
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Promoter
    MLV LTR
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172497-plasmid-map-sequence-id-340999

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  21. CAG-FLEX-MCS-WPRE-SV40 - GCH2

    Plasmid
    #230815
    Purpose
    AAV cloning vector for CRE-dependent expression under the CAG promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    230815-plasmid-map-sequence-id-466637

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  23. pCFD5_w

    Plasmid
    #112645
    Purpose
    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.
    Depositor
    Michael Boutros
    Insert
    U6:3-tRNA-sgRNA expression cassette
    Expression
    Insect
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112645-plasmid-map-sequence-id-217990

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  25. SpCas9-NG-ABE8e(V106W)

    Plasmid
    #198533
    Purpose
    Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198533-plasmid-map-sequence-id-388880

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  26. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid
    #139989
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139989-plasmid-map-sequence-id-275392

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  30. pEGFP-centrin-1

    Plasmid
    #72641
    Purpose
    The plasmid encodes centrin-1 with an N-terminal EGFP tag
    Depositor
    Michel Bornens
    Insert
    centrin-1 (CETN1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72641-plasmid-map-sequence-id-132790

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Showing: 8941 - 8970 of 169843 results