161,805 results

1. HDM_RSV_B_2019_F

   Plasmid

   #237359

   Purpose

   Expression plasmid for RSV B F from 2019

   Depositor

   Jesse Bloom

   Article

   Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

   Insert

   RSV B F from 2019 human codon optimized from Genbank Accesion PP352433
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. HDM_RSV_B_MN365362_F_K68Q

   Plasmid

   #237361

   Purpose

   Expression plasmid for RSV B F from MN365362

   Depositor

   Jesse Bloom

   Article

   Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

   Insert

   RSV B F human codon optimized from Genbank Accesion MN36536
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HDM_RSV_B1_G_31AACTdel

   Plasmid

   #237354

   Purpose

   Expression plasmid for RSV B1 G

   Depositor

   Jesse Bloom

   Article

   Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

   Insert

   RSV B1 G human codon optimized from Genbank Accesion AF013254
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   31 amino acid cytoplasmic tail deletion

   Promoter

   CMV

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. HDM_RSV_B_OQ279862_F_N201T

   Plasmid

   #237362

   Purpose

   Expression plasmid for RSV B F from OQ279862

   Depositor

   Jesse Bloom

   Article

   Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

   Insert

   RSV B F human codon optimized from Genbank Accesion OQ27986
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG GST Empty

   Plasmid

   #236735

   Purpose

   Empty bacterial expression plasmid for GST fusion proteins

   Depositor

   Melina Fan, Addgene Research Program

   Type

   Empty backbone

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAG GST EGFP

   Plasmid

   #236736

   Purpose

   Bacterial expression plasmid containing GST-tagged EGFP

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   EGFP
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   April 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. LSM081 SFFV-mCherry-SGc(stem-1(3xMS2)2-stops)cSG-EGFP (FLP-IN)

   Plasmid

   #233440

   Purpose

   SFFV driven LIDAR reporter with an additional stop codon on the stem loop

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   SGc(stem-1(3xMS2)2-stops)cSG-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKEK3395

   Plasmid

   #234079

   Purpose

   CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

   Depositor

   Karl Klose

   Article

   Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

   Insert

   YukonOFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pJ23100-BsaI

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDsRed1-CMV-Rat Arc-DsRed1-pA

   Plasmid

   #233049

   Purpose

   To Express a Rat Arc-DsRed fusion protein from a CMV promoter

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Neurobiol Learn Mem. 2015 Oct;124:34-47.

   Insert

   rat Arc-DsRed fusion (Arc Rat)
   

   Use

   Tags

   DsRed1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdest EGFP Neo

    Plasmid

    #223052

    Purpose

    EGFP gene expression in mammalian cells

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-22b(+) pCopA RBS30 mscarlet

    Plasmid

    #226370

    Purpose

    Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

    Depositor

    Urartu Seker

    Article

    Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

    Insert

    mScarlet
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Promoter

    copA promoter (pCopA)

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa

    Plasmid

    #221352

    Purpose

    Mammalian expression of Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa; to be used with Anti-EE [IPI-3D5EE_48.K] heavy chain (Plasmid 221351) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC

    Plasmid

    #221351

    Purpose

    Mammalian expression of Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC; to be used with Anti-EE [IPI-3D5EE_48.K] light chain (Plasmid 221352) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDONR221-ATP6V0D1

    Plasmid

    #166154

    Purpose

    Gateway entry vector for N. furzeri ATP6V0D1V

    Depositor

    Daniel Jarosz

    Article

    Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

    Insert

    ATP6V0D1V
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AiP12610 - pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610)

    Plasmid

    #191737

    Purpose

    AI plasmid ALIAS: AiP12610. Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Has Service

    AAV PHP.eB

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221-kcnk18

    Plasmid

    #209000

    Purpose

    Riboprobe generation

    Depositor

    GuangJun Zhang

    Article

    Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

    Insert

    kcnk18 (kcnk18 Zebrafish)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

    Plasmid

    #198450

    Purpose

    Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mc2 mCherry hPGK

    Plasmid

    #206231

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF3455

    Plasmid

    #144931

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF513 (ZNF513 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TT lux sensor

    Plasmid

    #199792

    Purpose

    Synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

    Insert

    TT lux Sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TFORF0088

    Plasmid

    #142455

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF408 (ZNF408 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CROP_C9_Puro_TOP2B

    Plasmid

    #183325

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets TOP2B gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    TOP2B
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CROP_C9_Puro_TOP2A

    Plasmid

    #183324

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets TOP2A gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    TOP2A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pB6-Gfap-T2A-tdTomato-FNF-DTA

    Plasmid

    #140271

    Purpose

    B6J Gfap-T2A-tdTomato allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    Gfap (Gfap Mouse)
    

    Use

    Mouse Targeting

    Tags

    C-terminal fusion of T2A-tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGDP1 uvrA

    Plasmid

    #112892

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    uvrA-like protein
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGDP1 stat

    Plasmid

    #112886

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    stat
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TXNRD1

    Plasmid

    #38863

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TXNRD1 (Txnrd1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. secretedBirA-8his

    Plasmid

    #32408

    Depositor

    Gavin Wright

    Article

    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

    Insert

    BirA
    

    Use

    Tags

    8 His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMSCV-loxp-dsRed-loxp-3xHA-Puro-WPRE

    Plasmid

    #32703

    Depositor

    Hans Clevers

    Article

    Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    Cre/Lox and Retroviral

    Tags

    3x HA tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. JD150

    Plasmid

    #229485

    Purpose

    Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, VA RNA I, E4orf6, L4-22k/33k
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter …

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge